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[172252_x_at]

Description H25K10.4 /REP_DB=WormBase Gene ID /WP=CE19936 /TR=Q9XU17 /GB=CAB07232.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_total > 44      4grp_total > 12      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 220
1 2 3 4 range
A 59.4
(-1.4)
A 		19.6
(-3)
A 		37.7
(-2.3)
A 		167.8
(3)
A 		148
B 106.5
(1.1)
A 		57
(-1.2)
A 		18.4
(-3)
A 		148.2
(3)
A 		130
C 17.4
(-2.7)
A 		45.5
(-1.9)
A 		6.2
(-3)
A 		226.3
(3)
M 		220
avr 61.1
(-1.5) 		40.7
(-2.3) 		20.8
(-3) 		180.8
(3) 		160

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 130
1 2 3 4 5 6 range
A 56.8
(-0.4)
A 		9.7
(-2.7)
A 		128.1
(3)
A 		4
(-3)
A 		64.5
(-0.1)
A 		5.4
(-2.9)
A 		124
B 84
(2)
A 		69.7
(1)
A 		69.6
(1)
A 		13.5
(-3)
A 		14.5
(-2.9)
A 		98.2
(3)
A 		85
C 17.2
(-2.8)
A 		133.5
(3)
A 		13
(-3)
A 		90.1
(0.8)
A 		57.1
(-0.8)
A 		41.3
(-1.6)
A 		121
avr 52.7
(-0.1) 		71
(3) 		70.2
(2.9) 		35.9
(-3) 		45.4
(-1.4) 		48.3
(-0.9) 		35
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 36
1 2 3 4 range
A 21.6
(2.1)
A 		4.3
(-3)
A 		9.9
(-1.3)
A 		24.6
(3)
A 		20
B 34
(3)
A 		14.1
(-1)
A 		4.3
(-3)
A 		31.6
(2.5)
A 		30
C 5.8
(-2.4)
A 		13.1
(-1.2)
A 		2.1
(-3)
A 		38.2
(3)
M 		36
avr 20.5
(0.5) 		10.5
(-1.8) 		5.4
(-3) 		31.5
(3) 		26

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 40
1 2 3 4 5 6 range
A 10.5
(-0.9)
A 		3.4
(-2.5)
A 		27.9
(3)
A 		1
(-3)
A 		19.5
(1.1)
A 		1.3
(-2.9)
A 		27
B 29.2
(1.7)
A 		23.2
(0.6)
A 		16.9
(-0.6)
A 		3.6
(-3)
A 		5.9
(-2.6)
A 		36.6
(3)
A 		33
C 6.4
(-2.5)
A 		40.7
(3)
A 		3.3
(-3)
A 		18.1
(-0.6)
A 		18.5
(-0.6)
A 		13.8
(-1.3)
A 		37
avr 15.4
(0.2) 		22.4
(3) 		16
(0.4) 		7.6
(-3) 		14.6
(-0.2) 		17.2
(0.9) 		15
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 172252_x_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.8609
Sequence Type Exemplar sequence
Representative Public ID H25K10.4 NCBI
Target Description H25K10.4 /REP_DB=WormBase Gene ID /WP=CE19936 /TR=Q9XU17 /GB=CAB07232.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_070522(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_070522 NCBI Caenorhabditis elegans H25K10.4 (H25K10.4) mRNA, complete cds. 11/11 None
GENEFINDER00000037545 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:16226008:16227598:1 11/11 None
H25K10.4 ENSEMBL cdna:known chromosome:CEL140:IV:16226008:16227598:1 gene:H25K10.4 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_070526 1/11 Cross Hyb Matching Probes A 
SNAP00000039182 1/11 Cross Hyb Matching Probes None
GENEFINDER00000039196 1/11 Cross Hyb Matching Probes None
F38C2.1 1/11 Cross Hyb Matching Probes A 
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:16226536-16227604(+) 93.62 93.62
Public Domain and Genome References
Gene Title
Ensembl H25K10.4
Entrez Gene 186780 Entrez gene
SwissProt Q9XU17 EMBL-EBI
WormBase CE19936 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB07232 Hypothetical protein H25K10.4 [Caenorhabditis elegans] ref|NP_502923.1| H25K10.4 [Caenorhabditis elegans] 7.0E-61
blast CAB05188 Hypothetical protein F38C2.1 [Caenorhabditis elegans] ref|NP_502927.1| F38C2.1 [Caenorhabditis elegans] 2.0E-50
blast AAY86271 Hypothetical protein R09E12.9 [Caenorhabditis elegans] 8.0E-47
Sequence
Target Sequence 
>C. ELEGANS:172252_X_AT
atggcttcttatactgctgaacaagcagaggccctccttaagccgcaatttgataaatac
gcaaaattcatcgacgatgaggtgtgggcggaggtagaagggtattatcatccaaacgga
gttctggttcataaggataaggaagcggtttatggaaataaagacatatcctcctacatt
gcaaagtttgcggaaggaaccggaaaatcggtgggaactactacaaacgccaagtacgaa
ggaaccggtgacttcctcattatcactgccgacttttcttctgaaactga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATGGCTTCTTATACTGCTGAACAAG 62 53 13 Antisense
GAACAAGCAGAGGCCCTCCTTAAGC 316 351 31 Antisense
CCCTCCTTAAGCCGCAATTTGATAA 122 261 44 Antisense
GAGGTGTGGGCGGAGGTAGAAGGGT 366 399 91 Antisense
GAAGGGTATTATCATCCAAACGGAG 531 333 109 Antisense
CATCCAAACGGAGTTCTGGTTCATA 255 209 121 Antisense
TAAAGACATATCCTCCTACATTGCA 238 641 171 Antisense
GACATATCCTCCTACATTGCAAAGT 213 371 175 Antisense
GTGGGAACTACTACAAACGCCAAGT 192 487 223 Antisense
AGGAACCGGTGACTTCCTCATTATC 353 53 252 Antisense
CTGCCGACTTTTCTTCTGAAACTGA 572 235 278 Antisense
Sequence Source Affymetrix Proprietary Database