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[172249_x_at]

Description B0391.9 /REP_DB=WormBase Gene ID /WP=CE17337 /TR=O62014 /GB=CAB03801.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_total > 90      4grp_total > 05      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 75
1 2 3 4 range
A 28.2
(1.2)
A 		32
(2)
A 		5.9
(-3)
A 		37.4
(3)
A 		32
B 45.7
(3)
A 		4.5
(-3)
A 		34.9
(1.4)
A 		44.4
(2.8)
A 		41
C 12.1
(-3)
A 		12.9
(-2.9)
A 		31.8
(-1.3)
A 		79.7
(3)
A 		68
avr 28.7
(-1) 		16.5
(-3) 		24.2
(-1.8) 		53.8
(3) 		37

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 65
1 2 3 4 5 6 range
A 13.2
(-1.6)
A 		4
(-3)
A 		16.9
(-1)
A 		42.4
(3)
A 		14.7
(-1.3)
A 		16.4
(-1.1)
A 		38
B 10.2
(-1.7)
A 		23.7
(0.8)
A 		8.4
(-2)
A 		8.5
(-2)
A 		3.1
(-3)
A 		35.5
(3)
A 		32
C 29.8
(-0.8)
A 		35.3
(-0.2)
A 		30
(-0.8)
A 		67.6
(3)
A 		47.5
(1)
A 		7.6
(-3)
A 		60
avr 17.7
(-3) 		21
(-2.1) 		18.4
(-2.8) 		39.5
(3) 		21.8
(-1.9) 		19.8
(-2.4) 		22
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 14
1 2 3 4 range
A 10.3
(3)
A 		6.9
(0.7)
A 		1.5
(-3)
A 		5.5
(-0.3)
A 		9
B 14.6
(3)
A 		1.1
(-3)
A 		8.1
(0.1)
A 		9.5
(0.7)
A 		14
C 4
(-2.8)
A 		3.7
(-3)
A 		11.1
(1.6)
A 		13.4
(3)
A 		10
avr 9.6
(3) 		3.9
(-3) 		6.9
(0.2) 		9.5
(2.9) 		6

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 14
1 2 3 4 5 6 range
A 2.4
(-2.4)
A 		1.4
(-3)
A 		3.7
(-1.5)
A 		10.8
(3)
A 		4.4
(-1.1)
A 		4
(-1.3)
A 		9
B 3.6
(-1.8)
A 		7.9
(0.3)
A 		2
(-2.6)
A 		2.3
(-2.5)
A 		1.3
(-3)
A 		13.2
(3)
A 		12
C 11
(0.9)
A 		10.8
(0.8)
A 		7.6
(-0.7)
A 		13.6
(2.2)
A 		15.4
(3)
A 		2.6
(-3)
A 		13
avr 5.7
(-1.3) 		6.7
(0.1) 		4.4
(-3) 		8.9
(3) 		7
(0.5) 		6.6
(-0.1) 		5
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 172249_x_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.14784
Sequence Type Exemplar sequence
Representative Public ID B0391.9 NCBI
Target Description B0391.9 /REP_DB=WormBase Gene ID /WP=CE17337 /TR=O62014 /GB=CAB03801.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_074475(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_074475 NCBI Caenorhabditis elegans B0391.9 (B0391.9) mRNA, complete cds. 11/11 None
SNAP00000015396 ENSEMBL cdna:SNAP chromosome:CEL140:V:15596046:15598218:-1 11/11 None
GENEFINDER00000015412 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:15596046:15596383:-1 11/11 None
B0391.9 ENSEMBL cdna:known chromosome:CEL140:V:15596046:15596383:-1 gene:B0391.9 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_075669 3/11 Cross Hyb Matching Probes A 
SNAP00000022309 4/11 Cross Hyb Matching Probes None
GENEFINDER00000022360 5/11 Cross Hyb Matching Probes None
Y73B3A.14 3/11 Cross Hyb Matching Probes A 
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:120808-121146(+) 95.49 95.49
chrV:15596045-15596383(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl B0391.9
Entrez Gene 181954 Entrez gene
SwissProt O62014 EMBL-EBI
WormBase CE17337 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB03801 Hypothetical protein B0391.9 [Caenorhabditis elegans] ref|NP_506876.1| B0391.9 [Caenorhabditis elegans] 2.0E-41
blast CAB03801 Hypothetical protein B0391.9 [Caenorhabditis elegans] ref|NP_506876.1| B0391.9 [Caenorhabditis elegans] 3.0E-37
blast AAK39352 Hypothetical protein Y73B3A.14 [Caenorhabditis elegans] ref|NP_508070.1| Y73B3A.14 [Caenorhabditis elegans] 5.0E-21
blast AAK39352 Hypothetical protein Y73B3A.14 [Caenorhabditis elegans] ref|NP_508070.1| Y73B3A.14 [Caenorhabditis elegans] 1.0E-20
blast CAE60032 Hypothetical protein CBG03540 [Caenorhabditis briggsae] 8.0E-19
blast CAB07208 Hypothetical protein F47H4.9 [Caenorhabditis elegans] ref|NP_507392.1| F47H4.9 [Caenorhabditis elegans] 8.0E-10
Protein Domains
Database ID Description E-Value
Pfam IPR002900 EMBL-EBI Protein of unknown function DUF38 8.3E-15
Pfam IPR002900 EMBL-EBI Protein of unknown function DUF38 7.2E-10
Sequence
Target Sequence 
>C. ELEGANS:172249_X_AT
atgccaatggagtatctctacaacttcgatcgatttgagcttcatctaatcagatttaat
ccggaagatgcaattaaggttagagatgttatctgcaaatctccaacattcgaatttgga
tatttcccggccatcgattttttcttccctgaagaggtcgcgagagtcttccagccagac
tacgaaggtggttcagaaggatcaatccgttaccgaaattccactgccgattttctcat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATGCCAATGGAGTATCTCTACAACT 416 41 13 Antisense
GGAGTATCTCTACAACTTCGATCGA 691 517 21 Antisense
ACTTCGATCGATTTGAGCTTCATCT 82 105 35 Antisense
GAGCTTCATCTAATCAGATTTAATC 51 389 49 Antisense
TATCTGCAAATCTCCAACATTCGAA 421 657 102 Antisense
GAATTTGGATATTTCCCGGCCATCG 500 325 124 Antisense
CATCGATTTTTTCTTCCCTGAAGAG 315 207 144 Antisense
GAAGAGGTCGCGAGAGTCTTCCAGC 581 337 163 Antisense
GTCTTCCAGCCAGACTACGAAGGTG 496 443 178 Antisense
GATCAATCCGTTACCGAAATTCCAC 522 421 212 Antisense
GAAATTCCACTGCCGATTTTCTCAT 120 365 227 Antisense
Sequence Source Affymetrix Proprietary Database