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[172248_x_at]

Description T20G5.8 /REP_DB=WormBase Gene ID /WP=CE00588 /TR=Q22614 /GB=CAA83009.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 30      6grp_high1_10_10 > 13      4grp_highest_8_8 > 01      4grp_high_8_8 > 54      6grp_highest_nonorm > 41      6grp_total > 22      4grp_total > 50      6grp_highest_sub > De      6grp_high1_sub > De      6grp_total_sub > Hat      6grp_rule_based > S2      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1057
1 2 3 4 range
A 622.5
(-1.1)
P 		1262.9
(3)
P 		1222.3
(2.7)
P 		331.5
(-3)
P 		931
B 391.6
(-2.8)
P 		1275.3
(3)
P 		1146.7
(2.2)
P 		364.2
(-3)
P 		911
C 560.1
(-1.7)
P 		1385.6
(3)
P 		1281.4
(2.4)
P 		328.6
(-3)
P 		1057
avr 524.7
(-1.9) 		1307.9
(3) 		1216.8
(2.4) 		341.4
(-3) 		967

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 931
1 2 3 4 5 6 range
A 813.3
(1.1)
P 		820.9
(1.2)
P 		1058.1
(3)
P 		534.3
(-1)
P 		640.9
(-0.2)
P 		281.3
(-3)
P 		777
B 1162.4
(3)
P 		1142.4
(2.9)
P 		743.7
(-0)
P 		671.3
(-0.6)
P 		612.6
(-1)
P 		335.1
(-3)
P 		827
C 938.7
(2)
P 		1079.9
(3)
P 		652.3
(-0)
P 		572.6
(-0.6)
P 		528.3
(-0.9)
P 		231.6
(-3)
P 		848
avr 971.5
(2.6) 		1014.4
(3) 		818
(1.4) 		592.7
(-0.5) 		593.9
(-0.4) 		282.7
(-3) 		732
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 398
1 2 3 4 range
A 226.6
(0.9)
P 		274.1
(2)
P 		319.5
(3)
P 		48.5
(-3)
P 		271
B 124.9
(-1.8)
P 		315
(3)
P 		265.7
(1.8)
P 		77.6
(-3)
P 		237
C 185.4
(-1)
P 		398.4
(2.3)
P 		446.6
(3)
P 		55.4
(-3)
P 		391
avr 179
(-0.5) 		329.2
(2.7) 		343.9
(3) 		60.5
(-3) 		283

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 336
1 2 3 4 5 6 range
A 149.9
(-0.8)
P 		291.9
(3)
P 		230.4
(1.3)
P 		135.5
(-1.2)
P 		193.4
(0.4)
P 		68.5
(-3)
P 		223
B 404.5
(3)
P 		380.3
(2.5)
P 		180.6
(-1.8)
P 		177.8
(-1.9)
P 		248.9
(-0.3)
P 		125
(-3)
P 		280
C 347.9
(3)
P 		329.5
(2.6)
P 		166
(-1)
P 		115.2
(-2.2)
P 		171
(-0.9)
P 		77.5
(-3)
P 		270
avr 300.8
(2.2) 		333.9
(3) 		192.3
(-0.5) 		142.8
(-1.7) 		204.4
(-0.2) 		90.3
(-3) 		244
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 172248_x_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.6016
Sequence Type Exemplar sequence
Representative Public ID T20G5.8 NCBI
Target Description T20G5.8 /REP_DB=WormBase Gene ID /WP=CE00588 /TR=Q22614 /GB=CAA83009.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_066857(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_066857 NCBI Caenorhabditis elegans T20G5.8 (T20G5.8) mRNA, complete cds. 11/11 None
SNAP00000033300 ENSEMBL cdna:SNAP chromosome:CEL140:III:10199248:10199478:1 11/11 None
GENEFINDER00000033341 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:10199248:10199478:1 11/11 None
T20G5.8 ENSEMBL cdna:known chromosome:CEL140:III:10199248:10199478:1 gene:T20G5.8 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:10199243-10199474(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl T20G5.8
Entrez Gene 188660 Entrez gene
SwissProt Q22614 EMBL-EBI
WormBase CE00588 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA83009 Hypothetical protein T20G5.8 [Caenorhabditis elegans] ref|NP_499258.1| T20G5.8 [Caenorhabditis elegans] 2.0E-29
blast CAA83008 Hypothetical protein T20G5.7 [Caenorhabditis elegans] ref|NP_499257.1| Downstream Of DAF-16 (regulated by DAF-16) family member (dod-6) [Caenorhabditis elegans] 5.0E-25
blast CAE64968 Hypothetical protein CBG09802 [Caenorhabditis briggsae] 2.0E-22
Protein Domains
Database ID Description E-Value
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 4.4E-7
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_499258.1 1 7-24
T20G5.8 1 7-24
SNAP00000033300 1 7-24
GENEFINDER00000033341 1 7-24
Sequence
Target Sequence 
>C. ELEGANS:172248_X_AT
tcctcgtcattttgtctgtttttgttgttcatacttcagctacatgtgctgatgatgaag
atggtcactgcgccgtcttcgctgaactttgcgacaatgctgacttcgccgact

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TCCTCGTCATTTTGTCTGTTTTTGT 25 593 44 Antisense
GTCATTTTGTCTGTTTTTGTTGTTC 59 469 49 Antisense
GTTTTTGTTGTTCATACTTCAGCTA 47 449 61 Antisense
TGTTGTTCATACTTCAGCTACATGT 261 563 66 Antisense
TGTTCATACTTCAGCTACATGTGCT 87 563 69 Antisense
ACTTCAGCTACATGTGCTGATGATG 380 103 76 Antisense
ATGATGAAGATGGTCACTGCGCCGT 283 45 95 Antisense
CCGTCTTCGCTGAACTTTGCGACAA 352 265 116 Antisense
GCTGAACTTTGCGACAATGCTGACT 578 295 124 Antisense
GAACTTTGCGACAATGCTGACTTCG 48 351 127 Antisense
TGCGACAATGCTGACTTCGCCGACT 120 583 133 Antisense
Sequence Source Affymetrix Proprietary Database