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[172231_x_at]

Description C25D7.2 /REP_DB=WormBase Gene ID /WP=CE08388 /TR=O17595 /GB=CAB02773.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_total > 99      4grp_total > 34      6grp_total_sub > Increase      6grp_rule_based > S5      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 46
1 2 3 4 range
A 3.1
(-3)
A 		8.5
(-1.8)
A 		4
(-2.8)
A 		30.9
(3)
A 		28
B 4.7
(-2.7)
A 		13.8
(3)
A 		8.1
(-0.6)
A 		4.2
(-3)
A 		10
C 15
(-3)
A 		49.3
(3)
A 		35.8
(0.6)
A 		28.2
(-0.7)
A 		34
avr 7.6
(-3) 		23.9
(3) 		16
(0.1) 		21.1
(2) 		16

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 99
1 2 3 4 5 6 range
A 5
(-2.8)
A 		5.9
(-2.8)
A 		2.5
(-3)
A 		7.7
(-2.7)
A 		96.9
(2.7)
A 		101.5
(3)
P 		99
B 5.9
(-3)
A 		20
(-1.2)
A 		37.7
(1.1)
A 		33.4
(0.5)
A 		52.6
(3)
P 		24.7
(-0.6)
A 		47
C 6.9
(-2.9)
A 		27.8
(-1.1)
A 		6.1
(-3)
A 		21.3
(-1.7)
A 		75.8
(3)
A 		74.9
(2.9)
P 		70
avr 5.9
(-3) 		17.9
(-2) 		15.4
(-2.2) 		20.8
(-1.7) 		75.1
(3) 		67
(2.3) 		69
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 13
1 2 3 4 range
A 1.1
(-3)
A 		1.8
(-1.8)
A 		1.1
(-3)
A 		4.5
(3)
A 		3
B 1.5
(-1.6)
A 		3.4
(3)
A 		1.9
(-0.6)
A 		0.9
(-3)
A 		3
C 5
(-2.9)
A 		14.2
(3)
A 		12.5
(1.9)
A 		4.8
(-3)
A 		9
avr 2.5
(-3) 		6.5
(3) 		5.2
(1.1) 		3.4
(-1.7) 		4

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 29
1 2 3 4 5 6 range
A 0.9
(-2.9)
A 		2.1
(-2.7)
A 		0.5
(-3)
A 		2
(-2.7)
A 		29.2
(3)
A 		24.7
(2.1)
P 		29
B 2.1
(-3)
A 		6.7
(-1.6)
A 		9.1
(-0.8)
A 		8.8
(-0.9)
A 		21.4
(3)
P 		9.2
(-0.8)
A 		19
C 2.6
(-2.7)
A 		8.5
(-1.2)
A 		1.6
(-3)
A 		4.3
(-2.3)
A 		24.5
(2.8)
A 		25.1
(3)
P 		24
avr 1.9
(-3) 		5.8
(-2) 		3.7
(-2.5) 		5
(-2.2) 		25
(3) 		19.7
(1.6) 		23
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 172231_x_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.13645
Sequence Type Exemplar sequence
Representative Public ID C25D7.2 NCBI
Target Description C25D7.2 /REP_DB=WormBase Gene ID /WP=CE08388 /TR=O17595 /GB=CAB02773.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_074301(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_074301 NCBI Caenorhabditis elegans C25D7.2 (C25D7.2) mRNA, complete cds. 11/11 A 
SNAP00000019703 ENSEMBL cdna:SNAP chromosome:CEL140:V:15036966:15038006:1 10/11 None
SNAP00000019705 ENSEMBL cdna:SNAP chromosome:CEL140:V:15040381:15041421:-1 11/11 None
GENEFINDER00000019718 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:15040381:15055800:-1 11/11 None
GENEFINDER00000019726 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:15036027:15038006:1 10/11 None
C25D7.14 ENSEMBL cdna:pseudogene chromosome:CEL140:V:15037023:15038006:1 gene:C25D7.14 10/11 A 
C25D7.2 ENSEMBL cdna:known chromosome:CEL140:V:15040381:15041421:-1 gene:C25D7.2 11/11 A 
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_074299 8/11 Cross Hyb Matching Probes A 
NM_074697 1/11 Cross Hyb Matching Probes A 
NM_074781 1/11 Cross Hyb Matching Probes A 
SNAP00000014724 1/11 Cross Hyb Matching Probes None
GENEFINDER00000014733 1/11 Cross Hyb Matching Probes None
SNAP00000019702 8/11 Cross Hyb Matching Probes None
GENEFINDER00000019725 8/11 Cross Hyb Matching Probes None
SNAP00000025148 1/11 Cross Hyb Matching Probes None
GENEFINDER00000025156 1/11 Cross Hyb Matching Probes None
C25D7.12 8/11 Cross Hyb Matching Probes A 
F21H7.5 1/11 Cross Hyb Matching Probes A 
F36D3.4 1/11 Cross Hyb Matching Probes A 
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:15036965-15038006(+) 99.88 99.88
chrV:15040380-15041421(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl C25D7.14
C25D7.2
Entrez Gene 180008 Entrez gene
SwissProt O17595 EMBL-EBI
WormBase CE08388 Wormbase
Functional Annotations
Gene Ontology
GO Molecular Function (view graph)
ID Description Evidence Links
5198 structural molecule activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB02774 Hypothetical protein C25D7.3 [Caenorhabditis elegans] ref|NP_506703.1| Sex determination and Dosage Compensation defect family member (sdc-3) [Caenorhabditis elegans] sp|P34706|SDC3_CAEEL Zinc finger protein sdc-3 0.0
blast AAB25348 Sdc-3=sex determination and dosage compensation control [Caenorhabditis elegans, Peptide, 2150 aa] gb|AAA28144.1| zinc finger protein 0.0
blast CAB02775 Hypothetical protein C25D7.4 [Caenorhabditis elegans] ref|NP_506704.1| C25D7.4 [Caenorhabditis elegans] 1.0E-143
blast CAB02773 Hypothetical protein C25D7.2 [Caenorhabditis elegans] ref|NP_506702.1| C25D7.2 [Caenorhabditis elegans] 2.0E-98
blast CAB02773 Hypothetical protein C25D7.2 [Caenorhabditis elegans] ref|NP_506702.1| C25D7.2 [Caenorhabditis elegans] 1.0E-97
blast CAB02773 Hypothetical protein C25D7.2 [Caenorhabditis elegans] ref|NP_506702.1| C25D7.2 [Caenorhabditis elegans] 2.0E-85
blast CAB07588 Hypothetical protein F21H7.5 [Caenorhabditis elegans] ref|NP_507098.1| F21H7.5 [Caenorhabditis elegans] 8.0E-80
blast CAB07588 Hypothetical protein F21H7.5 [Caenorhabditis elegans] ref|NP_507098.1| F21H7.5 [Caenorhabditis elegans] 7.0E-79
Protein Domains
Database ID Description E-Value
Pfam IPR000535 EMBL-EBI Major sperm protein (MSP) domain 3.5E-30
Pfam IPR002900 EMBL-EBI Protein of unknown function DUF38 1.2E-32
Pfam IPR001810 EMBL-EBI Cyclin-like F-box 8.5E-10
Pfam IPR000535 EMBL-EBI Major sperm protein (MSP) domain 3.5E-30
Pfam IPR005135 EMBL-EBI Endonuclease/exonuclease/phosphatase 3.4E-7
Pfam IPR000535 EMBL-EBI Major sperm protein (MSP) domain 3.5E-30
Sequence
Target Sequence 
>C. ELEGANS:172231_X_AT
gagcacggggagctcctaagtcggaatcgaagaagctgagacgtgacaaggacaagtcgt
cgaaatcagaaagatccagaagatcttcaaagtctaagaagtgcgacaagtcggccaaga
agtgtgacctgaacaaggcgaagaacctctgcccaccggtctcagttgtttccgatgcgt
ccatcaaatcaaattctggggagaagtccgagaaatcgaaggctctcaaattggttccca
atcagtcgaactacacattcccggagggacaggggactgcctcagtgaagacatccaccc
ttcgtgcttcacccaacaagctgccattcgcaacaacgggtggtgttcagacagttagca
tcgcgaacaacaccaagtcgagaaaggcgtttaaggtcaaaacctctgacaatcttctgt
accgtgtcaacccagtctttggattcgttgagccgggagacaagctttcgatcgatgtgc
tccgt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAGCACGGGGAGCTCCTAAGTCGGA 425 385 254 Antisense
AACAAGGCGAAGAACCTCTGCCCAC 326 133 385 Antisense
CACCGGTCTCAGTTGTTTCCGATGC 30 201 407 Antisense
GAAGGCTCTCAAATTGGTTCCCAAT 359 333 471 Antisense
AATCAGTCGAACTACACATTCCCGG 200 171 493 Antisense
GACTGCCTCAGTGAAGACATCCACC 485 375 528 Antisense
CAACAAGCTGCCATTCGCAACAACG 438 189 567 Antisense
GGTGTTCAGACAGTTAGCATCGCGA 505 497 595 Antisense
AAAACCTCTGACAATCTTCTGTACC 541 131 652 Antisense
TACCGTGTCAACCCAGTCTTTGGAT 31 647 673 Antisense
CAAGCTTTCGATCGATGTGCTCCGT 59 187 714 Antisense
Sequence Source Affymetrix Proprietary Database