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[172229_x_at]

Description T16A9.5 /REP_DB=WormBase Gene ID /WP=CE13728 /TR=Q22524 /GB=CAB00878.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_total > 88      4grp_total > 34      6grp_total_sub > Increase      6grp_rule_based > S6      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 69
1 2 3 4 range
A 39.2
(-3)
A 		49.9
(-0.9)
A 		64.2
(1.8)
A 		70.5
(3)
A 		31
B 33.8
(-2.9)
P 		91.2
(3)
M 		47.4
(-1.5)
A 		33.3
(-3)
A 		58
C 29
(-2.2)
A 		75.4
(3)
A 		22
(-3)
A 		63.9
(1.7)
A 		53
avr 34
(-3) 		72.2
(3) 		44.5
(-1.4) 		55.9
(0.4) 		38

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1193
1 2 3 4 5 6 range
A 83.2
(-2.7)
M 		29.8
(-3)
A 		31.7
(-3)
A 		90.6
(-2.7)
P 		581.3
(-0.2)
P 		1222.6
(3)
P 		1193
B 37.9
(-3)
A 		41.2
(-3)
A 		45.3
(-3)
A 		92.7
(-2.7)
P 		540.3
(0.1)
P 		1014.4
(3)
P 		977
C 36.3
(-3)
A 		55.6
(-2.9)
A 		44
(-3)
A 		80.7
(-2.7)
P 		534.2
(0)
P 		1024
(3)
P 		988
avr 52.5
(-2.9) 		42.2
(-3) 		40.3
(-3) 		88
(-2.7) 		551.9
(-0.1) 		1087
(3) 		1047
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 15
1 2 3 4 range
A 14.3
(0.7)
A 		10.8
(-2.5)
A 		16.8
(3)
A 		10.3
(-3)
A 		7
B 10.8
(-1.6)
P 		22.5
(3)
M 		11
(-1.5)
A 		7.1
(-3)
A 		15
C 9.6
(-2.2)
A 		21.7
(3)
A 		7.7
(-3)
A 		10.8
(-1.7)
A 		14
avr 11.6
(-1.5) 		18.3
(3) 		11.8
(-1.4) 		9.4
(-3) 		9

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 371
1 2 3 4 5 6 range
A 15.3
(-2.8)
M 		10.6
(-2.9)
A 		6.9
(-3)
A 		23
(-2.7)
P 		175.4
(0.5)
P 		297.7
(3)
P 		291
B 13.2
(-3)
A 		13.7
(-3)
A 		11
(-3)
A 		24.6
(-2.8)
P 		219.5
(0.4)
P 		378.3
(3)
P 		367
C 13.4
(-3)
A 		17
(-2.9)
A 		11.2
(-3)
A 		16.2
(-2.9)
P 		172.8
(-0.1)
P 		342.8
(3)
P 		332
avr 14
(-2.9) 		13.8
(-2.9) 		9.7
(-3) 		21.3
(-2.8) 		189.2
(0.3) 		339.6
(3) 		330
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 172229_x_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.13258
Sequence Type Exemplar sequence
Representative Public ID T16A9.5 NCBI
Target Description T16A9.5 /REP_DB=WormBase Gene ID /WP=CE13728 /TR=Q22524 /GB=CAB00878.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_074118(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_074118 NCBI Caenorhabditis elegans T16A9.5 (T16A9.5) mRNA, complete cds. 11/11 A 
SNAP00000032389 ENSEMBL cdna:SNAP chromosome:CEL140:V:14216216:14217517:-1 11/11 None
GENEFINDER00000032395 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:14216216:14217517:-1 11/11 None
T16A9.5 ENSEMBL cdna:known chromosome:CEL140:V:14216216:14217588:-1 gene:T16A9.5 11/11 A 
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_069637 3/11 Cross Hyb Matching Probes A 
SNAP00000037371 3/11 Cross Hyb Matching Probes None
GENEFINDER00000037381 3/11 Cross Hyb Matching Probes None
Y69E1A.1 3/11 Cross Hyb Matching Probes A 
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:10947244-10948712(-) 94.94 94.94
chrV:14216215-14217517(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl T16A9.5
Entrez Gene 179924 Entrez gene
SwissProt Q22524 EMBL-EBI
WormBase CE13728 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB00878 Hypothetical protein T16A9.5 [Caenorhabditis elegans] ref|NP_506519.1| T16A9.5 [Caenorhabditis elegans] 1.0E-125
blast CAA22256 Hypothetical protein Y69E1A.1 [Caenorhabditis elegans] ref|NP_502038.1| Y69E1A.1 [Caenorhabditis elegans] 1.0E-122
blast NP_491044 W03D8.9 [Caenorhabditis elegans] gb|AAK21499.1| Hypothetical protein W03D8.9 [Caenorhabditis elegans] 9.0E-91
Protein Domains
Database ID Description E-Value
Pfam IPR008574 EMBL-EBI Caenorhabditis elegans protein of unknown function DUF856 1.0E-126
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_506519.1 6 122-144,151-170,185-207,212-234,249-271,292-314
T16A9.5 6 122-144,151-170,185-207,212-234,249-271,292-314
SNAP00000032389 6 122-144,151-170,185-207,212-234,249-271,292-314
GENEFINDER00000032395 6 122-144,151-170,185-207,212-234,249-271,292-314
Sequence
Target Sequence 
>C. ELEGANS:172229_X_AT
ttcggatggtcttcaattctgcttctcggagctggaattgcaagcattattggaggatat
gtgctttctccaattttcggaattttcattggaagaggtggatcggccattttggcaact
cttgccattcccgccttcggtgcctatcaactgaacaatgaggatggaacgacatcggga
acacgtttccaacttttggttttggcattggttcaaggacttttgatggggcactcaatt
tcatacacttatctgagtgctcaaccactcggtttcgttactccattggtcattgctttt
gcgtacccattgattgccgggcaagttggaaccgcccgtactccattgcttggcggagct
gttggagccgcattcggaactcaacttgtgctcggacttgtctctggatcactatcgttc
tcttacttgctcttgtctgctctctacagtgcagcttctggtgctattcttcaagttgcc
ttcaagaacttgactgctcagaacagaatccacatgtaccagattcttttggtctcttcg
ttcctcttctcgaaggccctcgtatacggat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TTCGGATGGTCTTCAATTCTGCTTC 388 693 379 Antisense
GATATGTGCTTTCTCCAATTTTCGG 227 425 434 Antisense
GGGCACTCAATTTCATACACTTATC 485 493 607 Antisense
TGCTCAACCACTCGGTTTCGTTACT 125 575 636 Antisense
ACTCCATTGGTCATTGCTTTTGCGT 104 99 658 Antisense
TTGCGTACCCATTGATTGCCGGGCA 288 597 677 Antisense
TTCGGAACTCAACTTGTGCTCGGAC 546 689 751 Antisense
GACTTGTCTCTGGATCACTATCGTT 540 371 773 Antisense
TACAGTGCAGCTTCTGGTGCTATTC 121 645 823 Antisense
GGTGCTATTCTTCAAGTTGCCTTCA 557 499 838 Antisense
TTCTCGAAGGCCCTCGTATACGGAT 54 693 925 Antisense
Sequence Source Affymetrix Proprietary Database