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[172224_x_at]

Description F46A8.9 /REP_DB=WormBase Gene ID /WP=CE18682 /TR=Q9XTM7 /GB=CAB04395.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_total > 99      4grp_total > 56      6grp_total_sub > Increase      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 54
1 2 3 4 range
A 11.4
(-2.4)
A 		12.5
(-2.3)
A 		59.8
(3)
A 		5.9
(-3)
A 		54
B 11.6
(-1)
A 		11.7
(-1)
A 		22.3
(3)
A 		6.3
(-3)
A 		16
C 15.2
(1.3)
A 		17.1
(3)
A 		16.7
(2.6)
A 		10.4
(-3)
A 		7
avr 12.7
(-1.8) 		13.8
(-1.5) 		32.9
(3) 		7.5
(-3) 		25

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 75
1 2 3 4 5 6 range
A 11
(-2.5)
A 		18.1
(-2)
A 		5.2
(-3)
A 		29.6
(-1)
A 		28
(-1.2)
A 		79.9
(3)
P 		75
B 7.8
(-2.7)
A 		19
(-1.1)
A 		13.3
(-1.9)
A 		6
(-3)
A 		47.1
(3)
A 		41.5
(2.2)
A 		41
C 11.4
(-2.3)
A 		5.3
(-3)
A 		11.4
(-2.3)
A 		25.3
(-0.7)
A 		56.7
(3)
A 		12.5
(-2.2)
A 		51
avr 10.1
(-3) 		14.1
(-2.3) 		10
(-3) 		20.3
(-1.2) 		43.9
(2.9) 		44.6
(3) 		35
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 15
1 2 3 4 range
A 4.1
(-1.7)
A 		2.7
(-2.3)
A 		15.6
(3)
A 		0.9
(-3)
A 		15
B 3.7
(0.7)
A 		2.9
(-0.5)
A 		5.2
(3)
A 		1.3
(-3)
A 		4
C 5
(1.8)
A 		4.9
(1.7)
A 		5.8
(3)
A 		1.8
(-3)
A 		4
avr 4.3
(-0.6) 		3.5
(-1.3) 		8.9
(3) 		1.3
(-3) 		8

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 18
1 2 3 4 5 6 range
A 2
(-2.7)
A 		6.5
(-1.2)
A 		1.1
(-3)
A 		7.5
(-0.9)
A 		8.5
(-0.6)
A 		19.5
(3)
P 		18
B 2.7
(-2.6)
A 		6.3
(-1.4)
A 		3.2
(-2.5)
A 		1.6
(-3)
A 		19.1
(3)
A 		15.5
(1.8)
A 		18
C 4.2
(-2.1)
A 		1.6
(-3)
A 		2.9
(-2.5)
A 		5.1
(-1.7)
A 		18.3
(3)
A 		4.2
(-2.1)
A 		17
avr 3
(-2.7) 		4.8
(-1.9) 		2.4
(-3) 		4.7
(-1.9) 		15.3
(3) 		13.1
(2) 		13
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 172224_x_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.1120
Sequence Type Exemplar sequence
Representative Public ID F46A8.9 NCBI
Target Description F46A8.9 /REP_DB=WormBase Gene ID /WP=CE18682 /TR=Q9XTM7 /GB=CAB04395.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_060488(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_060488 NCBI Caenorhabditis elegans F46A8.9 (F46A8.9) mRNA, complete cds. 11/11 None
F46A8.9 ENSEMBL cdna:known chromosome:CEL140:I:11260362:11260992:1 gene:F46A8.9 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_068099 2/11 Cross Hyb Matching Probes A 
NM_068100 1/11 Cross Hyb Matching Probes A 
SNAP00000009681 1/11 Cross Hyb Matching Probes None
SNAP00000009682 1/11 Cross Hyb Matching Probes None
GENEFINDER00000009696 2/11 Cross Hyb Matching Probes None
GENEFINDER00000009699 1/11 Cross Hyb Matching Probes None
K08D10.8.2 2/11 Cross Hyb Matching Probes A 
K08D10.8.1 2/11 Cross Hyb Matching Probes A 
K08D10.7 1/11 Cross Hyb Matching Probes A 
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:11260292-11260923(+) 100.0 100.0
chrIV:4162520-4166062(+) 94.16 94.16
Public Domain and Genome References
Gene Title
Ensembl F46A8.9
Entrez Gene 185825 Entrez gene
SwissProt Q9XTM7 EMBL-EBI
WormBase CE18682 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB04395 Hypothetical protein F46A8.9 [Caenorhabditis elegans] emb|CAB04313.1| Hypothetical protein F46A8.9 [Caenorhabditis elegans] ref|NP_492889.1| F46A8.9 [Caenorhabditis elegans] 4.0E-85
blast AAA98028 Hypothetical protein K08D10.8 [Caenorhabditis elegans] ref|NP_500500.1| K08D10.8 [Caenorhabditis elegans] 8.0E-60
blast AAA98029 Hypothetical protein K08D10.7 [Caenorhabditis elegans] ref|NP_500501.1| K08D10.7 [Caenorhabditis elegans] 1.0E-55
Protein Domains
Database ID Description E-Value
Pfam IPR005552 EMBL-EBI Scramblase 1.1E-42
Sequence
Target Sequence 
>C. ELEGANS:172224_X_AT
gagatcgcttagcttgacgtctgcggccaaatcacttgaggaggtcgagccggaggacga
ttcgaagaaaggcgagccacatgccaaggctgcaggattcggaagaggtggaggtggtgg
aaagagactcccagcggcaagatccacgccaatggtcattccgaaccaggtggcagcgat
gccggttcagatgactggcttcgtccaacttgccaccaacaacatcctcagcgcaatcgc
ccataccaactccctgatggttgtccaatgcatcgaaccactcgagatcttcaccggatt
cgaaaccccgaatcgctacgtggtccacgacatgtactgccgtcctctgctctattgtat
ggagagatcaaacgtctttgcccgtcagtaccacggaaacaatcgtaaattgtccgcctc
t

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAGATCGCTTAGCTTGACGTCTGCG 267 397 42 Antisense
GTCTGCGGCCAAATCACTTGAGGAG 262 469 60 Antisense
AGGAGGTCGAGCCGGAGGACGATTC 553 55 80 Antisense
AAAGGCGAGCCACATGCCAAGGCTG 283 121 109 Antisense
AAGATCCACGCCAATGGTCATTCCG 178 155 180 Antisense
GTTCAGATGACTGGCTTCGTCCAAC 356 443 226 Antisense
ATACCAACTCCCTGATGGTTGTCCA 516 21 284 Antisense
GAGATCTTCACCGGATTCGAAACCC 186 395 325 Antisense
CCGTCCTCTGCTCTATTGTATGGAG 509 265 381 Antisense
GAGAGATCAAACGTCTTTGCCCGTC 420 395 403 Antisense
AAACAATCGTAAATTGTCCGCCTCT 161 129 438 Antisense
Sequence Source Affymetrix Proprietary Database