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[172221_x_at]

Description ZK84.4 /REP_DB=WormBase Gene ID /WP=CE02927 /TR=Q23632 /GB=AAC48206.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_total > 46      4grp_total > 19      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 106
1 2 3 4 range
A 18.8
(-2)
A 		25
(-1.7)
A 		2.5
(-3)
A 		104.1
(3)
P 		102
B 23.8
(-0.4)
A 		47.7
(3)
P 		5.2
(-3)
A 		44.4
(2.5)
M 		43
C 3
(-3)
A 		44.2
(-0.7)
A 		4.1
(-2.9)
A 		108.5
(3)
P 		106
avr 15.2
(-2.2) 		39
(-0.4) 		3.9
(-3) 		85.7
(3) 		82

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 78
1 2 3 4 5 6 range
A 9.1
(-3)
A 		18.8
(-1.7)
A 		24.1
(-1)
A 		19.2
(-1.6)
A 		12.1
(-2.6)
A 		53.2
(3)
P 		44
B 42.1
(3)
P 		39.5
(2.6)
M 		1.9
(-3)
A 		3.8
(-2.7)
A 		24.1
(0.3)
A 		36.9
(2.2)
P 		40
C 31.1
(-0.8)
A 		79.5
(3)
P 		3
(-3)
A 		29
(-1)
A 		33.3
(-0.6)
A 		36.8
(-0.3)
P 		77
avr 27.4
(-0.1) 		45.9
(3) 		9.7
(-3) 		17.3
(-1.7) 		23.2
(-0.8) 		42.3
(2.4) 		36
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 18
1 2 3 4 range
A 6.8
(-0.5)
A 		5.4
(-1)
A 		0.6
(-3)
A 		15.2
(3)
P 		15
B 7.6
(0.6)
A 		11.8
(3)
P 		1.2
(-3)
A 		9.5
(1.7)
M 		11
C 1
(-3)
A 		12.7
(1.1)
A 		1.4
(-2.9)
A 		18.3
(3)
P 		17
avr 5.1
(-1.2) 		10
(1) 		1.1
(-3) 		14.3
(3) 		13

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 24
1 2 3 4 5 6 range
A 1.7
(-3)
A 		6.7
(-0.3)
A 		5.2
(-1.1)
A 		4.9
(-1.3)
A 		3.7
(-1.9)
A 		13
(3)
P 		11
B 14.7
(3)
P 		13.2
(2.4)
M 		0.5
(-3)
A 		1
(-2.8)
A 		9.8
(0.9)
A 		13.8
(2.6)
P 		14
C 11.5
(-0.3)
A 		24.2
(3)
P 		0.8
(-3)
A 		5.8
(-1.7)
A 		10.8
(-0.4)
A 		12.3
(-0.1)
P 		23
avr 9.3
(0.4) 		14.7
(3) 		2.2
(-3) 		3.9
(-2.2) 		8.1
(-0.2) 		13
(2.2) 		13
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 172221_x_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.5346
Sequence Type Exemplar sequence
Representative Public ID ZK84.4 NCBI
Target Description ZK84.4 /REP_DB=WormBase Gene ID /WP=CE02927 /TR=Q23632 /GB=AAC48206.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_062798(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_062798 NCBI Caenorhabditis elegans ZK84.4 (ZK84.4) mRNA, complete cds. 11/11 None
ZK84.4 ENSEMBL cdna:known chromosome:CEL140:II:6008414:6008718:-1 gene:ZK84.4 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_074099 8/11 Cross Hyb Matching Probes A 
NM_064243 1/11 Cross Hyb Matching Probes A 
GENEFINDER00000021618 6/11 Cross Hyb Matching Probes None
W05H5.2 1/11 Cross Hyb Matching Probes A 
AH10.4 8/11 Cross Hyb Matching Probes A 
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:503239-503532(+) 76.71 92.24
chrII:6008410-6008715(-) 68.95 100.0
chrIV:7251447-7251739(-) 67.58 97.26
chrV:14139935-14140228(-) 68.49 98.63
Public Domain and Genome References
Ensembl ZK84.4
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast NP_495199 ZK84.4 [Caenorhabditis elegans] 4.0E-22
blast CAB02684 Hypothetical protein AH10.4 [Caenorhabditis elegans] ref|NP_506500.1| AH10.4 [Caenorhabditis elegans] 1.0E-21
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_495199.1 1 42-64
ZK84.4 1 42-64
Sequence
Target Sequence 
>C. ELEGANS:172221_X_AT
atgcacgagggacctttggaaataagatttttgaagttgtttaatttccctgatttctgt
ccatcgaaagaacttcaggaactccaacaaaggaagaagagaacttgctgcacaacggaa
caatttttaat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATGCACGAGGGACCTTTGGAAATAA 183 41 13 Antisense
TTTGAAGTTGTTTAATTTCCCTGAT 368 657 42 Antisense
AGTTGTTTAATTTCCCTGATTTCTG 205 63 47 Antisense
TTAATTTCCCTGATTTCTGTCCATC 426 683 53 Antisense
TTTCCCTGATTTCTGTCCATCGAAA 206 671 57 Antisense
GATTTCTGTCCATCGAAAGAACTTC 370 425 64 Antisense
CGAAAGAACTTCAGGAACTCCAACA 407 223 77 Antisense
GAACTTCAGGAACTCCAACAAAGGA 197 345 82 Antisense
GAAGAAGAGAACTTGCTGCACAACG 206 341 105 Antisense
GAACTTGCTGCACAACGGAACAATT 531 349 113 Antisense
GCTGCACAACGGAACAATTTTTAAT 622 295 119 Antisense
Sequence Source Affymetrix Proprietary Database