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[172218_x_at]

Description T20G5.7 /REP_DB=WormBase Gene ID /WP=CE00587 /TR=Q22613 /GB=CAA83008.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 40      6grp_high1_10_10 > 02      4grp_highest_8_8 > 56      4grp_high_8_8 > 34      6grp_highest_nonorm > 05      6grp_total > 34      4grp_total > 45      6grp_highest_sub > De      6grp_cgc > 21      6grp_high1_sub > De      6grp_cgc_sub > De      6grp_total_sub > Decrease      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 3122
1 2 3 4 range
A 9861.1
(3)
P 		8203.3
(-0.9)
P 		7301.7
(-3)
P 		7586
(-2.3)
P 		2559
B 9399.2
(3)
P 		8857.8
(1.7)
P 		7295.3
(-2.2)
P 		6988.4
(-3)
P 		2411
C 9326.1
(3)
P 		8681.5
(1.5)
P 		6739
(-3)
P 		7064.2
(-2.2)
P 		2587
avr 9528.8
(3) 		8580.9
(0.6) 		7112
(-3) 		7212.9
(-2.7) 		2417

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 3851
1 2 3 4 5 6 range
A 7078.6
(2.3)
P 		7231.9
(2.8)
P 		7308.2
(3)
P 		6638.6
(1.1)
P 		5216.6
(-3)
P 		6369.1
(0.3)
P 		2092
B 7890.5
(3)
P 		7330.4
(1.3)
P 		7737.7
(2.5)
P 		5867.5
(-3)
P 		6578.3
(-0.9)
P 		6020.5
(-2.5)
P 		2023
C 9068
(3)
P 		6658.9
(-1.6)
P 		7161.8
(-0.6)
P 		5904.9
(-3)
P 		6740.2
(-1.4)
P 		6498.2
(-1.9)
P 		3163
avr 8012.4
(3) 		7073.7
(-0) 		7402.6
(1) 		6137
(-3) 		6178.4
(-2.9) 		6295.9
(-2.5) 		1875
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 2480
1 2 3 4 range
A 3590.4
(3)
P 		1780.7
(-1.4)
P 		1908.8
(-1.1)
P 		1110.4
(-3)
P 		2480
B 2997.3
(3)
P 		2187.7
(-0.2)
P 		1690.5
(-2.2)
P 		1489.2
(-3)
P 		1508
C 3087.2
(3)
P 		2496
(1.1)
P 		2348.7
(0.7)
P 		1191.1
(-3)
P 		1896
avr 3225
(3) 		2154.8
(-0.3) 		1982.7
(-0.8) 		1263.6
(-3) 		1961

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 2174
1 2 3 4 5 6 range
A 1304.9
(-3)
P 		2572
(3)
P 		1591.6
(-1.6)
P 		1683.7
(-1.2)
P 		1574.1
(-1.7)
P 		1550.7
(-1.8)
P 		1267
B 2745.5
(3)
P 		2440
(1.5)
P 		1879
(-1.4)
P 		1554.1
(-3)
P 		2672.7
(2.6)
P 		2245
(0.5)
P 		1191
C 3361.1
(3)
P 		2031.8
(-0.7)
P 		1822.8
(-1.2)
P 		1187.5
(-3)
P 		2181
(-0.3)
P 		2175.3
(-0.3)
P 		2174
avr 2470.5
(3) 		2347.9
(2.3) 		1764.5
(-1.3) 		1475.1
(-3) 		2142.6
(1) 		1990.3
(0.1) 		995
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 172218_x_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.8032
Sequence Type Exemplar sequence
Representative Public ID T20G5.7 NCBI
Target Description T20G5.7 /REP_DB=WormBase Gene ID /WP=CE00587 /TR=Q22613 /GB=CAA83008.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_066856(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_066856 NCBI Caenorhabditis elegans Downstream Of DAF-16 (regulated by DAF-16) family member (dod-6) (dod-6) mRNA, complete cds. 11/11 None
SNAP00000033299 ENSEMBL cdna:SNAP chromosome:CEL140:III:10197645:10197883:1 11/11 None
GENEFINDER00000033344 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:10197645:10197883:1 11/11 None
T20G5.7 ENSEMBL cdna:known chromosome:CEL140:III:10197605:10198242:1 gene:T20G5.7 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:10197640-10197879(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol dod-6
Ensembl T20G5.7
Entrez Gene 176433 Entrez gene
SwissProt Q22613 EMBL-EBI
WormBase CE00587 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA83008 Hypothetical protein T20G5.7 [Caenorhabditis elegans] ref|NP_499257.1| Downstream Of DAF-16 (regulated by DAF-16) family member (dod-6) [Caenorhabditis elegans] 4.0E-29
blast CAA83009 Hypothetical protein T20G5.8 [Caenorhabditis elegans] ref|NP_499258.1| T20G5.8 [Caenorhabditis elegans] 5.0E-25
blast CAE64968 Hypothetical protein CBG09802 [Caenorhabditis briggsae] 5.0E-25
Protein Domains
Database ID Description E-Value
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 2.3E-6
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_499257.1 1 7-25
T20G5.7 1 7-25
SNAP00000033299 1 7-25
GENEFINDER00000033344 1 7-25
Sequence
Target Sequence 
>C. ELEGANS:172218_X_AT
gctgcttgcgctgatgctgaagatggtcactgtgccgtcttcg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GCTGCTTGCGCTGATGCTGAAGATG 343 297 82 Antisense
CTGCTTGCGCTGATGCTGAAGATGG 624 237 83 Antisense
GCTTGCGCTGATGCTGAAGATGGTC 704 305 85 Antisense
TTGCGCTGATGCTGAAGATGGTCAC 523 701 87 Antisense
GCGCTGATGCTGAAGATGGTCACTG 373 287 89 Antisense
GCTGATGCTGAAGATGGTCACTGTG 695 295 91 Antisense
GATGCTGAAGATGGTCACTGTGCCG 114 415 94 Antisense
TGCTGAAGATGGTCACTGTGCCGTC 252 579 96 Antisense
GCTGAAGATGGTCACTGTGCCGTCT 121 297 97 Antisense
CTGAAGATGGTCACTGTGCCGTCTT 118 237 98 Antisense
GAAGATGGTCACTGTGCCGTCTTCG 198 339 100 Antisense
Sequence Source Affymetrix Proprietary Database