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[172204_at]

Description T22H2.4 /REP_DB=WormBase Gene ID /WP=CE14002 /TR=O18111 /GB=CAB04751.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 39      6grp_high1_10_10 > 83      4grp_highest_8_8 > 15      4grp_high_8_8 > 14      6grp_highest_nonorm > 81      6grp_total > 74      4grp_total > 09      6grp_highest_sub > 04      6grp_high1_sub > 04      6grp_rule_based > S4      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 379
1 2 3 4 range
A 193.8
(-3)
P 		269.1
(-0.9)
P 		246.1
(-1.5)
P 		405.7
(3)
P 		212
B 179.1
(-3)
P 		270.5
(-1.2)
P 		220.1
(-2.2)
P 		485.9
(3)
P 		307
C 216.6
(-2.4)
P 		178
(-3)
P 		190.6
(-2.8)
P 		557.2
(3)
P 		379
avr 196.5
(-3) 		239.2
(-2.1) 		218.9
(-2.5) 		482.9
(3) 		286

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 278
1 2 3 4 5 6 range
A 158.9
(-2.7)
P 		143.5
(-3)
P 		419.3
(3)
P 		421.6
(3)
P 		376.1
(2)
P 		294.4
(0.3)
P 		278
B 244
(-3)
P 		249.5
(-2.7)
P 		321.1
(0.7)
P 		349
(2)
P 		370.3
(3)
P 		312.8
(0.3)
P 		126
C 200.5
(-3)
P 		315.7
(0.1)
P 		220.7
(-2.4)
P 		420.2
(3)
P 		291.9
(-0.5)
P 		349.7
(1.1)
P 		220
avr 201.1
(-3) 		236.2
(-1.9) 		320.4
(0.7) 		396.9
(3) 		346.1
(1.4) 		319
(0.6) 		196
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 53
1 2 3 4 range
A 70.6
(3)
P 		58.4
(-3)
P 		64.3
(-0.1)
P 		59.4
(-2.5)
P 		12
B 57.1
(-2.3)
P 		66.8
(-1.2)
P 		51
(-3)
P 		103.5
(3)
P 		53
C 71.7
(-0.1)
P 		51.2
(-3)
P 		66.4
(-0.9)
P 		93.9
(3)
P 		43
avr 66.5
(-1.3) 		58.8
(-3) 		60.6
(-2.6) 		85.6
(3) 		27

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 121
1 2 3 4 5 6 range
A 29.3
(-3)
P 		51
(-1.5)
P 		91.3
(1.4)
P 		106.9
(2.5)
P 		113.5
(3)
P 		71.7
(0)
P 		84
B 84.9
(-2.4)
P 		83
(-2.6)
P 		78
(-3)
P 		92.4
(-1.8)
P 		150.4
(3)
P 		116.6
(0.2)
P 		72
C 74.3
(-1.2)
P 		96.3
(1)
P 		56.2
(-3)
P 		84.5
(-0.2)
P 		94.5
(0.8)
P 		117.1
(3)
P 		61
avr 62.8
(-3) 		76.8
(-1.5) 		75.2
(-1.7) 		94.6
(0.4) 		119.5
(3) 		101.8
(1.1) 		57
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 172204_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.1448
Sequence Type Exemplar sequence
Representative Public ID T22H2.4 NCBI
Target Description T22H2.4 /REP_DB=WormBase Gene ID /WP=CE14002 /TR=O18111 /GB=CAB04751.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_060577(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_060577 NCBI Caenorhabditis elegans T22H2.4 (T22H2.4) mRNA, complete cds. 11/11 None
SNAP00000038041 ENSEMBL cdna:SNAP chromosome:CEL140:I:11702783:11703607:-1 10/11 None
T22H2.4 ENSEMBL cdna:known chromosome:CEL140:I:11702844:11703119:-1 gene:T22H2.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:11702774-11703050(-) 49.28 100.0
Public Domain and Genome References
Gene Title
Ensembl T22H2.4
Entrez Gene 188766 Entrez gene
SwissProt O18111 EMBL-EBI
WormBase CE14002 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB04751 Hypothetical protein T22H2.4 [Caenorhabditis elegans] ref|NP_492978.1| T22H2.4 [Caenorhabditis elegans] 1.0E-14
Protein Domains
Database ID Description E-Value
Pfam IPR005560 EMBL-EBI Protein of unknown function DUF326 1.5E-4
Trans Membrane
ID Number Of Domains Domain Boundaries
SNAP00000038041 1 4-26
Sequence
Target Sequence 
>C. ELEGANS:172204_AT
aaaagcaaattttcagatccaagaaatcccaaaatgcttccaacatcgttgctggtgtgt
tctaccggaatccccattgaatgctccgctgagctt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AAAAGCAAATTTTCAGATCCAAGAA 159 131 163 Antisense
GATCCAAGAAATCCCAAAATGCTTC 564 413 178 Antisense
GAAATCCCAAAATGCTTCCAACATC 58 363 185 Antisense
CCAAAATGCTTCCAACATCGTTGCT 124 277 191 Antisense
GCTTCCAACATCGTTGCTGGTGTGT 561 305 198 Antisense
AACATCGTTGCTGGTGTGTTCTACC 631 137 204 Antisense
ATCGTTGCTGGTGTGTTCTACCGGA 16 37 207 Antisense
TGTTCTACCGGAATCCCCATTGAAT 674 559 220 Antisense
TACCGGAATCCCCATTGAATGCTCC 335 649 225 Antisense
CGGAATCCCCATTGAATGCTCCGCT 213 243 228 Antisense
CCCCATTGAATGCTCCGCTGAGCTT 480 261 234 Antisense
Sequence Source Affymetrix Proprietary Database