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[172194_x_at]

Description ZK262.4 /REP_DB=WormBase Gene ID /WP=CE20427 /TR=Q9U1P0 /GB=CAB63419.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_total > 98      4grp_total > 44      6grp_total_sub > Increase      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 13
1 2 3 4 range
A 6.6
(3)
A 		3.6
(-0.4)
A 		1.3
(-3)
A 		4.2
(0.3)
A 		5
B 7
(-0.3)
A 		14.1
(3)
A 		1.2
(-2.9)
A 		1
(-3)
A 		13
C 2.9
(2.4)
A 		3.1
(3)
A 		1.7
(-1.4)
A 		1.2
(-3)
A 		2
avr 5.5
(1.5) 		6.9
(3) 		1.4
(-3) 		2.1
(-2.2) 		6

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 25
1 2 3 4 5 6 range
A 3.7
(-2.2)
A 		3.5
(-2.3)
A 		1.9
(-2.7)
A 		0.6
(-3)
A 		24.9
(3)
A 		15.5
(0.7)
A 		24
B 2.3
(-2.7)
A 		1.4
(-2.9)
A 		1.8
(-2.8)
A 		0.9
(-3)
A 		2.6
(-2.6)
A 		25.9
(3)
A 		25
C 1.3
(-3)
A 		1.5
(-2.9)
A 		21.1
(2.4)
A 		2
(-2.8)
A 		19.1
(1.9)
A 		23.2
(3)
A 		22
avr 2.4
(-2.6) 		2.1
(-2.7) 		8.3
(-0.9) 		1.2
(-3) 		15.5
(1.2) 		21.5
(3) 		20
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 3
1 2 3 4 range
A 2.4
(3)
A 		0.8
(-1.6)
A 		0.3
(-3)
A 		0.6
(-2.1)
A 		2
B 2.2
(0.6)
A 		3.5
(3)
A 		0.3
(-2.8)
A 		0.2
(-3)
A 		3
C 1
(3)
A 		0.9
(2.3)
A 		0.6
(-0)
A 		0.2
(-3)
A 		1
avr 1.9
(3) 		1.7
(2.3) 		0.4
(-2.6) 		0.3
(-3) 		2

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 10
1 2 3 4 5 6 range
A 0.7
(-2.5)
A 		1.3
(-2)
A 		0.4
(-2.8)
A 		0.1
(-3)
A 		7.5
(3)
A 		3.8
(-0)
A 		7
B 0.8
(-2.6)
A 		0.5
(-2.8)
A 		0.4
(-2.9)
A 		0.2
(-3)
A 		1.1
(-2.4)
A 		9.7
(3)
A 		10
C 0.5
(-2.9)
A 		0.4
(-3)
A 		5.4
(1.1)
A 		0.4
(-3)
A 		6.2
(1.7)
A 		7.8
(3)
A 		7
avr 0.7
(-2.6) 		0.7
(-2.6) 		2.1
(-1.3) 		0.2
(-3) 		4.9
(1.1) 		7.1
(3) 		7
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 172194_x_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.14464
Sequence Type Exemplar sequence
Representative Public ID ZK262.4 NCBI
Target Description ZK262.4 /REP_DB=WormBase Gene ID /WP=CE20427 /TR=Q9U1P0 /GB=CAB63419.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_075204(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_075204 NCBI Caenorhabditis elegans ZK262.4 (ZK262.4) mRNA, complete cds. 11/11 None
SNAP00000013661 ENSEMBL cdna:SNAP chromosome:CEL140:V:18426457:18426726:-1 10/11 None
GENEFINDER00000013674 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:18426457:18426781:-1 11/11 None
ZK262.4 ENSEMBL cdna:known chromosome:CEL140:V:18426457:18426781:-1 gene:ZK262.4 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_001029086 1/11 Cross Hyb Matching Probes None
SNAP00000034642 1/11 Cross Hyb Matching Probes None
GENEFINDER00000034651 1/11 Cross Hyb Matching Probes None
Y51A2B.6a 1/11 Cross Hyb Matching Probes A 
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:18426456-18426781(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl ZK262.4
Entrez Gene 191268 Entrez gene
SwissProt Q9U1P0 EMBL-EBI
WormBase CE20427 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB63419 Hypothetical protein ZK262.4 [Caenorhabditis elegans] ref|NP_507605.1| ZK262.4 [Caenorhabditis elegans] 3.0E-38
blast CAB63419 Hypothetical protein ZK262.4 [Caenorhabditis elegans] ref|NP_507605.1| ZK262.4 [Caenorhabditis elegans] 4.0E-34
blast CAA16394 Hypothetical protein Y51A2B.6 [Caenorhabditis elegans] ref|NP_001024257.1| Y51A2B.6 [Caenorhabditis elegans] 9.0E-12
blast CAB05258 Hypothetical protein M162.7 [Caenorhabditis elegans] ref|NP_507838.1| M162.7 [Caenorhabditis elegans] 1.0E-11
blast CAA16394 Hypothetical protein Y51A2B.6 [Caenorhabditis elegans] ref|NP_001024257.1| Y51A2B.6 [Caenorhabditis elegans] 1.0E-11
Sequence
Target Sequence 
>C. ELEGANS:172194_X_AT
tttccggttttcggactcacagctgccttcgagccggatgtactgctctccccaccagct
ccattcttctcgttcaagtacatctccacaattaatgatattgctaaaggaatcgatctt
caagttgatttgatgaatagagagattccaaaacttattggagatgtggcacattatcaa
atgt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TTTCCGGTTTTCGGACTCACAGCTG 557 667 22 Antisense
CCTTCGAGCCGGATGTACTGCTCTC 562 265 47 Antisense
ATTCTTCTCGTTCAAGTACATCTCC 618 9 84 Antisense
TTCTCGTTCAAGTACATCTCCACAA 271 693 88 Antisense
GTTCAAGTACATCTCCACAATTAAT 698 443 93 Antisense
GTACATCTCCACAATTAATGATATT 206 461 99 Antisense
GATATTGCTAAAGGAATCGATCTTC 288 427 118 Antisense
AAGGAATCGATCTTCAAGTTGATTT 315 161 128 Antisense
GAATAGAGAGATTCCAAAACTTATT 580 321 156 Antisense
AAACTTATTGGAGATGTGGCACATT 73 129 172 Antisense
GGAGATGTGGCACATTATCAAATGT 120 519 181 Antisense
Sequence Source Affymetrix Proprietary Database