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[172193_at]

Description T12D8.5 /REP_DB=WormBase Gene ID /WP=CE16402 /TR=O45783 /GB=CAB03345.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 89      6grp_high1_10_10 > 98      4grp_highest_8_8 > 47      4grp_high_8_8 > 11      6grp_highest_nonorm > 78      6grp_total > 96      4grp_total > 14      6grp_highest_sub > In      6grp_high1_sub > In      6grp_total_sub > Increase      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 2057
1 2 3 4 range
A 1981.1
(1.1)
P 		658.7
(-2.8)
P 		590.9
(-3)
P 		2641
(3)
P 		2050
B 1292.6
(-0.9)
P 		1058.7
(-1.6)
P 		584
(-3)
P 		2570.3
(3)
P 		1986
C 2249.3
(3)
P 		966.1
(-2)
P 		712.3
(-3)
P 		2198.5
(2.8)
P 		1537
avr 1841
(1) 		894.5
(-2.1) 		629.1
(-3) 		2469.9
(3) 		1841

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1553
1 2 3 4 5 6 range
A 810.3
(-3)
P 		1062.1
(-1.7)
P 		1530.7
(0.6)
P 		1708.5
(1.5)
P 		1808.7
(2)
P 		2012.6
(3)
P 		1202
B 1485.8
(-0.6)
P 		889
(-3)
P 		1117.4
(-2.1)
P 		1768.1
(0.6)
P 		2231.5
(2.5)
P 		2363.6
(3)
P 		1475
C 1562.4
(-0.4)
P 		1142
(-3)
P 		1349
(-1.7)
P 		1388.6
(-1.5)
P 		1790.8
(1)
P 		2106.7
(3)
P 		965
avr 1286.2
(-1.6) 		1031
(-3) 		1332.4
(-1.4) 		1621.7
(0.1) 		1943.7
(1.8) 		2161
(3) 		1130
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 609
1 2 3 4 range
A 721.3
(3)
P 		143
(-3)
P 		154.5
(-2.9)
P 		386.6
(-0.5)
P 		578
B 412.2
(1)
P 		261.5
(-1.2)
P 		135.3
(-3)
P 		547.7
(3)
P 		412
C 744.6
(3)
P 		277.8
(-2.6)
P 		248.3
(-3)
P 		370.7
(-1.5)
P 		496
avr 626
(3) 		227.4
(-2.4) 		179.4
(-3) 		435
(0.4) 		447

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 757
1 2 3 4 5 6 range
A 149.4
(-3)
P 		377.7
(0.5)
P 		333.4
(-0.2)
P 		433.3
(1.3)
P 		545.8
(3)
P 		490
(2.2)
P 		396
B 517
(-0.7)
P 		295.9
(-2.8)
P 		271.3
(-3)
P 		468.3
(-1.1)
P 		906.6
(3)
P 		881.4
(2.8)
P 		635
C 579.1
(1.2)
P 		348.5
(-2)
P 		343.3
(-2.1)
P 		279.3
(-3)
P 		579.5
(1.2)
P 		705.2
(3)
P 		426
avr 415.2
(-1.4) 		340.7
(-2.6) 		316
(-3) 		393.6
(-1.8) 		677.3
(2.8) 		692.2
(3) 		376
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 172193_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.6182
Sequence Type Exemplar sequence
Representative Public ID T12D8.5 NCBI
Target Description T12D8.5 /REP_DB=WormBase Gene ID /WP=CE16402 /TR=O45783 /GB=CAB03345.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_067414(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_067414 NCBI Caenorhabditis elegans T12D8.5 (T12D8.5) mRNA, complete cds. 11/11 None
SNAP00000041265 ENSEMBL cdna:SNAP chromosome:CEL140:III:13625548:13626044:-1 11/11 None
GENEFINDER00000041277 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:13625548:13626044:-1 11/11 None
T12D8.5.1 ENSEMBL cdna:known chromosome:CEL140:III:13625235:13626047:-1 gene:T12D8.5 11/11 None
T12D8.5.2 ENSEMBL cdna:known chromosome:CEL140:III:13625486:13626045:-1 gene:T12D8.5 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:13625543-13625672(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl T12D8.5
Entrez Gene 176797 Entrez gene
SwissProt O45783 EMBL-EBI
WormBase CE33832 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB03345 Hypothetical protein T12D8.5 [Caenorhabditis elegans] ref|NP_499815.2| T12D8.5 [Caenorhabditis elegans] 2.0E-71
blast CAE71347 Hypothetical protein CBG18250 [Caenorhabditis briggsae] 2.0E-42
Sequence
Target Sequence 
>C. ELEGANS:172193_AT
gtcaggttccgccaatgcgtaaggctagccgttacgatcatttgaaaatggcagccaaca
aaattgtcgagtcgacgagtgagctgaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTCAGGTTCCGCCAATGCGTAAGGC 90 465 23 Antisense
GGTTCCGCCAATGCGTAAGGCTAGC 129 451 27 Antisense
TCCGCCAATGCGTAAGGCTAGCCGT 57 597 30 Antisense
GCCAATGCGTAAGGCTAGCCGTTAC 475 277 33 Antisense
ATGCGTAAGGCTAGCCGTTACGATC 494 39 37 Antisense
GTAAGGCTAGCCGTTACGATCATTT 27 463 41 Antisense
AGGCTAGCCGTTACGATCATTTGAA 146 53 44 Antisense
CTAGCCGTTACGATCATTTGAAAAT 639 223 47 Antisense
AAATGGCAGCCAACAAAATTGTCGA 312 119 68 Antisense
TGGCAGCCAACAAAATTGTCGAGTC 319 545 71 Antisense
TTGTCGAGTCGACGAGTGAGCTGAA 38 679 86 Antisense
Sequence Source Affymetrix Proprietary Database