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[172180_x_at]

Description Y69A2AL.A /REP_DB=WormBase Gene ID /WP=CE25546 /CHR=4 /FEA=Sanger Annotation /DEF=(ST.LOUIS) protein_id:AAF60860.1
Cluster 6grp_total > 10      4grp_total > 63      6grp_cgc > 64      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 25
1 2 3 4 range
A 17.3
(0.2)
A 		16.6
(0.1)
A 		27.7
(3)
A 		5.1
(-3)
A 		23
B 26.1
(3)
A 		16.2
(0)
A 		9.5
(-2)
A 		6.1
(-3)
A 		20
C 6.6
(-1.9)
A 		3
(-3)
A 		12
(-0.3)
A 		22.9
(3)
A 		20
avr 16.7
(3) 		11.9
(-2.4) 		16.4
(2.7) 		11.4
(-3) 		5

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 35
1 2 3 4 5 6 range
A 4.4
(-2.4)
A 		20.6
(0.3)
A 		25.7
(1.2)
A 		15.6
(-0.5)
A 		36.3
(3)
A 		1
(-3)
A 		35
B 18.6
(0)
A 		23
(0.9)
A 		33.7
(3)
A 		3.1
(-3)
A 		17.1
(-0.3)
A 		11.2
(-1.4)
A 		31
C 14.7
(0.3)
A 		23.6
(3)
A 		7.4
(-2)
A 		4.1
(-3)
A 		20.3
(2)
A 		12.8
(-0.3)
A 		20
avr 12.6
(-1.2) 		22.4
(2.2) 		22.3
(2.2) 		7.6
(-3) 		24.6
(3) 		8.3
(-2.8) 		17
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 8
1 2 3 4 range
A 6.3
(2.2)
A 		3.6
(-0.3)
A 		7.2
(3)
A 		0.7
(-3)
A 		7
B 8.3
(3)
A 		4
(-0.7)
A 		2.2
(-2.2)
A 		1.3
(-3)
A 		7
C 2.2
(-0.6)
A 		0.9
(-3)
A 		4.2
(3)
A 		3.9
(2.5)
A 		3
avr 5.6
(3) 		2.8
(-1.7) 		4.5
(1.2) 		2
(-3) 		4

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 11
1 2 3 4 5 6 range
A 0.8
(-2.7)
A 		7.3
(1)
A 		5.6
(0)
A 		4
(-0.9)
A 		10.9
(3)
A 		0.3
(-3)
A 		11
B 6.5
(1.6)
A 		7.6
(2.5)
A 		8.2
(3)
A 		0.8
(-3)
A 		7
(2)
A 		4.2
(-0.2)
A 		7
C 5.4
(1.3)
A 		7.2
(3)
A 		1.9
(-2)
A 		0.8
(-3)
A 		6.6
(2.4)
A 		4.3
(0.3)
A 		6
avr 4.2
(-0.8) 		7.4
(2.2) 		5.2
(0.1) 		1.9
(-3) 		8.2
(3) 		2.9
(-2) 		6
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 172180_x_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.19187
Sequence Type Exemplar sequence
Representative Public ID Y69A2AL.A NCBI
Target Description Y69A2AL.A /REP_DB=WormBase Gene ID /WP=CE25546 /CHR=4 /FEA=Sanger Annotation /DEF=(ST.LOUIS) protein_id:AAF60860.1
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_067808(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_067808 NCBI Caenorhabditis elegans GRounDhog (hedgehog-like family) family member (grd-16) (grd-16) mRNA, complete cds. 11/11 None
GENEFINDER00000035000 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:2476698:2479283:1 11/11 None
Y69A2AL.1 ENSEMBL cdna:known chromosome:CEL140:IV:2476698:2480216:1 gene:Y69A2AL.1 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:2478503-2479188(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol grd-16
Ensembl Y69A2AL.1
Entrez Gene 190533 Entrez gene
SwissProt Q9N2Z2 EMBL-EBI
WormBase CE35134 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAF60860 Groundhog (hedgehog-like family) protein 16 [Caenorhabditis elegans] ref|NP_500209.2| GRounDhog (hedgehog-like family) family member (grd-16) [Caenorhabditis elegans] 0.0
blast AAF60860 Groundhog (hedgehog-like family) protein 16 [Caenorhabditis elegans] ref|NP_500209.2| GRounDhog (hedgehog-like family) family member (grd-16) [Caenorhabditis elegans] 1.0E-173
blast CAE74706 Hypothetical protein CBG22521 [Caenorhabditis briggsae] 1.0E-149
blast CAE74706 Hypothetical protein CBG22521 [Caenorhabditis briggsae] 1.0E-110
Protein Domains
Database ID Description E-Value
Pfam IPR007284 EMBL-EBI Protein of unknown function DUF398 2.8E-8
Sequence
Target Sequence 
>C. ELEGANS:172180_X_AT
tccgggggaatattaatcgacaaattcctgcgattcccctgccttcgcccttctcggtcg
cctacggcaagccttcatttgcaattgcacgggaagaagatggaaaatgctacacaaatc
gatttggatacc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TCCGGGGGAATATTAATCGACAAAT 644 593 19 Antisense
AATCGACAAATTCCTGCGATTCCCC 245 169 33 Antisense
CCTACGGCAAGCCTTCATTTGCAAT 108 269 79 Antisense
ACGGCAAGCCTTCATTTGCAATTGC 138 97 82 Antisense
CAAGCCTTCATTTGCAATTGCACGG 461 185 86 Antisense
GCCTTCATTTGCAATTGCACGGGAA 33 269 89 Antisense
TTTGCAATTGCACGGGAAGAAGATG 564 665 96 Antisense
GCAATTGCACGGGAAGAAGATGGAA 93 323 99 Antisense
GAAGAAGATGGAAAATGCTACACAA 250 341 111 Antisense
GAAAATGCTACACAAATCGATTTGG 699 351 121 Antisense
TGCTACACAAATCGATTTGGATACC 644 579 126 Antisense
Sequence Source Affymetrix Proprietary Database