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[172177_x_at]

Description Y71H2AL.C /REP_DB=WormBase Gene ID /WP=CE25579 /CHR=3 /FEA=Sanger Annotation /DEF=(ST.LOUIS) protein_id:AAF60872.1
Cluster 6grp_highest_10_10 > 70      6grp_high1_10_10 > 17      4grp_highest_8_8 > 42      4grp_high_8_8 > 48      6grp_highest_nonorm > 80      6grp_total > 06      4grp_total > 63      6grp_highest_sub > De      6grp_total_sub > Decrease      6grp_high1_sub > De      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 184
1 2 3 4 range
A 353.7
(3)
P 		350.5
(2.8)
P 		257.9
(-3)
P 		280.3
(-1.6)
P 		96
B 435.9
(3)
P 		287.9
(-3)
P 		339.9
(-0.9)
P 		340.2
(-0.9)
P 		148
C 442.1
(3)
P 		329
(-0.7)
P 		411.2
(2)
P 		259.1
(-3)
P 		183
avr 410.6
(3) 		322.5
(-1.5) 		336.3
(-0.8) 		293.2
(-3) 		117

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 200
1 2 3 4 5 6 range
A 247.4
(2.2)
P 		233.2
(1.4)
P 		260.9
(3)
P 		193.1
(-0.8)
P 		154.3
(-3)
P 		157.2
(-2.8)
P 		107
B 239.7
(3)
P 		231.6
(2.5)
P 		200.3
(0.5)
P 		144.5
(-3)
P 		172.7
(-1.2)
P 		195.5
(0.2)
P 		95
C 344.6
(3)
P 		172
(-2.5)
P 		204.9
(-1.5)
P 		157.3
(-3)
P 		267.7
(0.5)
P 		163.1
(-2.8)
P 		187
avr 277.2
(3) 		212.3
(-0.5) 		222
(0) 		165
(-3) 		198.2
(-1.2) 		171.9
(-2.6) 		112
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 105
1 2 3 4 range
A 128.8
(3)
P 		76.1
(-0.6)
P 		67.4
(-1.2)
P 		41
(-3)
P 		88
B 139
(3)
P 		71.1
(-3)
P 		78.8
(-2.3)
P 		72.5
(-2.9)
P 		68
C 146.4
(3)
P 		94.6
(-0)
P 		143.3
(2.8)
P 		43.7
(-3)
P 		103
avr 138.1
(3) 		80.6
(-1) 		96.5
(0.1) 		52.4
(-3) 		86

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 96
1 2 3 4 5 6 range
A 45.6
(-2)
P 		82.9
(3)
P 		56.8
(-0.5)
P 		49
(-1.6)
P 		46.6
(-1.9)
P 		38.3
(-3)
P 		45
B 83.4
(3)
P 		77.1
(2.2)
P 		48.6
(-1.6)
P 		38.3
(-3)
P 		70.2
(1.2)
P 		72.9
(1.6)
P 		45
C 127.7
(3)
P 		52.5
(-1.7)
P 		52.2
(-1.7)
P 		31.6
(-3)
P 		86.6
(0.4)
P 		54.6
(-1.6)
P 		96
avr 85.6
(3) 		70.8
(1.1) 		52.5
(-1.3) 		39.6
(-3) 		67.8
(0.7) 		55.3
(-1) 		46
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 172177_x_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.18914
Sequence Type Exemplar sequence
Representative Public ID Y71H2AL.C NCBI
Target Description Y71H2AL.C /REP_DB=WormBase Gene ID /WP=CE25579 /CHR=3 /FEA=Sanger Annotation /DEF=(ST.LOUIS) protein_id:AAF60872.1
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_065200(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_065200 NCBI Caenorhabditis elegans Y71H2AL.1 (Y71H2AL.1) mRNA, complete cds. 11/11 A 
GENEFINDER00000036794 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:2681544:2686450:-1 11/11 None
Y71H2AL.1 ENSEMBL cdna:known chromosome:CEL140:III:2681519:2686450:-1 gene:Y71H2AL.1 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:2681543-2681700(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl Y71H2AL.1
Entrez Gene 175385 Entrez gene
SwissProt Q9N2Y1 EMBL-EBI
WormBase CE27032 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE_2:CFAAFFX.26992.1.S1_AT similar to hepatocellular carcinoma antigen gene 520 cfa
HG-U133_PLUS_2:206149_AT hepatocellular carcinoma antigen gene 520 hs
HG-U133A:206149_AT hepatocellular carcinoma antigen gene 520 hs
HG-FOCUS:206149_AT hepatocellular carcinoma antigen gene 520 hs
HG-U133A_2:206149_AT hepatocellular carcinoma antigen gene 520 hs
HU35KSUBD:RC_AA435717_AT hepatocellular carcinoma antigen gene 520 hs
U133_X3P:G11545810_3P_AT hepatocellular carcinoma antigen gene 520 hs
HG-U95AV2:33007_AT hepatocellular carcinoma antigen gene 520 hs
HG-U95B:58951_AT hepatocellular carcinoma antigen gene 520 hs
MOE430B:1431130_AT RIKEN cDNA 2010110P09 gene mm
MOUSE430_2:1431130_AT RIKEN cDNA 2010110P09 gene mm
MG-U74CV2:167236_AT PREDICTED: calcineurin B homologous protein 2 [Mus musculus], mRNA sequence mm
Gene Ontology
GO Molecular Function (view graph)
ID Description Evidence Links
5509 calcium ion binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAF60872 Hypothetical protein Y71H2AL.1 [Caenorhabditis elegans] ref|NP_497601.1| Y71H2AL.1 [Caenorhabditis elegans] 1.0E-109
blast CAE64998 Hypothetical protein CBG09836 [Caenorhabditis briggsae] 1.0E-105
blast CAE61065 Hypothetical protein CBG04814 [Caenorhabditis briggsae] 6.0E-46
Protein Domains
Database ID Description E-Value
Pfam IPR002048 EMBL-EBI Calcium-binding EF-hand 7.3E-8
Pfam IPR002048 EMBL-EBI Calcium-binding EF-hand 4.8E-9
Sequence
Target Sequence 
>C. ELEGANS:172177_X_AT
atgcgtgaagctgatagagacggagacggattcatcacttttcaagaattctgcaacgca
atggaaaagacagatat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATGCGTGAAGCTGATAGAGACGGAG 369 39 13 Antisense
GAAGCTGATAGAGACGGAGACGGAT 271 343 19 Antisense
AGAGACGGAGACGGATTCATCACTT 585 69 28 Antisense
GACGGAGACGGATTCATCACTTTTC 294 377 31 Antisense
CGGAGACGGATTCATCACTTTTCAA 67 243 33 Antisense
GAGACGGATTCATCACTTTTCAAGA 585 393 35 Antisense
GACGGATTCATCACTTTTCAAGAAT 175 377 37 Antisense
TTCATCACTTTTCAAGAATTCTGCA 87 689 43 Antisense
CACTTTTCAAGAATTCTGCAACGCA 613 195 48 Antisense
TCAAGAATTCTGCAACGCAATGGAA 665 629 54 Antisense
GCAACGCAATGGAAAAGACAGATAT 255 319 65 Antisense
Sequence Source Affymetrix Proprietary Database