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[172153_x_at]

Description B0350.2B /REP_DB=WormBase Gene ID /WP=CE06703 /GEN=unc-44 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 41      6grp_high1_10_10 > 15      4grp_highest_8_8 > 41      4grp_high_8_8 > 50      6grp_highest_nonorm > 02      6grp_total > 15      4grp_total > 53      6grp_highest_sub > De      6grp_cgc > 32      6grp_high1_sub > De      6grp_cgc_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S1      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 4704
1 2 3 4 range
A 5640.4
(3)
P 		3517.1
(0.1)
P 		3316.1
(-0.2)
P 		1260.1
(-3)
P 		4380
B 5099.5
(3)
P 		4108.9
(1.5)
P 		3256.2
(0.2)
P 		1093.8
(-3)
P 		4006
C 4702.1
(3)
P 		3667.2
(1.4)
P 		3082.6
(0.4)
P 		936.2
(-3)
P 		3766
avr 5147.3
(3) 		3764.4
(1) 		3218.3
(0.1) 		1096.7
(-3) 		4051

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 3085
1 2 3 4 5 6 range
A 4111.7
(2.5)
P 		4330.4
(3)
P 		1786.7
(-2.9)
P 		2099.7
(-2.1)
P 		2034.7
(-2.3)
P 		1730.2
(-3)
P 		2600
B 3646.7
(3)
P 		3019.5
(1.3)
P 		2306
(-0.7)
P 		1894.9
(-1.8)
P 		1469.1
(-3)
P 		1496.4
(-2.9)
P 		2178
C 3844.5
(3)
P 		3216
(1.5)
P 		3115.4
(1.3)
P 		1497.3
(-2.4)
P 		2047.9
(-1.1)
P 		1245.4
(-3)
P 		2599
avr 3867.6
(3) 		3522
(2.1) 		2402.7
(-0.7) 		1830.6
(-2.1) 		1850.6
(-2.1) 		1490.7
(-3) 		2377
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1896
1 2 3 4 range
A 2053.7
(3)
P 		763.5
(-1.1)
P 		866.9
(-0.8)
P 		184.5
(-3)
P 		1869
B 1626.2
(3)
P 		1014.8
(0.4)
P 		754.5
(-0.8)
P 		233.1
(-3)
P 		1393
C 1556.5
(3)
P 		1054.3
(0.8)
P 		1074.4
(0.9)
P 		157.9
(-3)
P 		1399
avr 1745.5
(3) 		944.2
(-0.1) 		898.6
(-0.3) 		191.8
(-3) 		1554

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1239
1 2 3 4 5 6 range
A 758
(-1.1)
P 		1540.1
(3)
P 		389.1
(-3)
P 		532.5
(-2.3)
P 		614
(-1.8)
P 		421.3
(-2.8)
P 		1151
B 1268.9
(3)
P 		1005.1
(0.9)
P 		560
(-2.5)
P 		501.9
(-3)
P 		596.9
(-2.3)
P 		558
(-2.6)
P 		767
C 1425
(3)
P 		981.3
(0.6)
P 		792.9
(-0.4)
P 		301.1
(-3)
P 		662.7
(-1.1)
P 		416.9
(-2.4)
P 		1124
avr 1150.6
(2.8) 		1175.5
(3) 		580.7
(-1.9) 		445.2
(-3) 		624.5
(-1.5) 		465.4
(-2.8) 		730
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 172153_x_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.11069
Sequence Type Exemplar sequence
Representative Public ID B0350.2B NCBI
Target Description B0350.2B /REP_DB=WormBase Gene ID /WP=CE06703 /GEN=unc-44 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001026097(11), NM_068497(11), NM_001026096(11), NM_068500(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001026097 NCBI Caenorhabditis elegans UNCoordinated family member (unc-44) (unc-44) mRNA, complete cds. 11/11 None
NM_068497 NCBI Caenorhabditis elegans UNCoordinated family member (unc-44) (unc-44) mRNA, complete cds. 11/11 None
NM_001026096 NCBI Caenorhabditis elegans UNCoordinated family member (unc-44) (unc-44) mRNA, complete cds. 11/11 None
NM_068500 NCBI Caenorhabditis elegans UNCoordinated family member (unc-44) (unc-44) mRNA, complete cds. 11/11 None
SNAP00000023739 ENSEMBL cdna:SNAP chromosome:CEL140:IV:5981117:5985165:1 11/11 None
GENEFINDER00000023990 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:5975560:5985165:1 11/11 None
B0350.2c ENSEMBL cdna:known chromosome:CEL140:IV:5975560:5987438:1 gene:B0350.2 11/11 A 
B0350.2f ENSEMBL cdna:known chromosome:CEL140:IV:5975560:6004843:1 gene:B0350.2 11/11 None
B0350.2d.1 ENSEMBL cdna:known chromosome:CEL140:IV:5979302:5985358:1 gene:B0350.2 11/11 None
B0350.2b.2 ENSEMBL cdna:known chromosome:CEL140:IV:5981051:5985366:1 gene:B0350.2 11/11 None
B0350.2b.1 ENSEMBL cdna:known chromosome:CEL140:IV:5981054:5985366:1 gene:B0350.2 11/11 None
B0350.2d.2 ENSEMBL cdna:known chromosome:CEL140:IV:5982419:5985165:1 gene:B0350.2 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_001026095 6/11 Cross Hyb Matching Probes None
NM_171907 8/11 Cross Hyb Matching Probes None
B0350.2a 6/11 Cross Hyb Matching Probes None
B0350.2e 8/11 Cross Hyb Matching Probes None
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
177366 7 4 NM_001026095 175561_s_at (11), 176663_s_at (11)
NM_001026096 172153_x_at (11), 173125_s_at (11)
NM_001026097 171738_x_at (9), 172123_x_at (11), 172153_x_at (11), 173125_s_at (11), 174033_at (11), 181045_at (11)
NM_068497 171738_x_at (9), 172123_x_at (11), 172153_x_at (11), 173125_s_at (11)
NM_068500 172153_x_at (11), 173125_s_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:5975565-5985171(+) 100.0 100.0
Public Domain and Genome References
Ensembl B0350.2
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAA93447 Uncoordinated protein 44, isoform f [Caenorhabditis elegans] ref|NP_001021268.1| UNCoordinated family member (unc-44) [Caenorhabditis elegans] 0.0
blast AAB41827 AO13 ankyrin [Caenorhabditis elegans] 0.0
blast AAA93444 Uncoordinated protein 44, isoform c [Caenorhabditis elegans] ref|NP_500898.1| UNCoordinated family member (unc-44) [Caenorhabditis elegans] gb|AAB41828.1| AO66 ankyrin [Caenorhabditis elegans] 0.0
blast AAV34805 Uncoordinated protein 44, isoform b [Caenorhabditis elegans] ref|NP_500901.2| UNCoordinated family member (unc-44) [Caenorhabditis elegans] 0.0
blast AAV34806 Uncoordinated protein 44, isoform d [Caenorhabditis elegans] ref|NP_001021267.1| UNCoordinated family member (unc-44) [Caenorhabditis elegans] 0.0
blast AAB41826 AO49 ankyrin [Caenorhabditis elegans] 0.0
Protein Domains
Database ID Description E-Value
scop a.4.5.MarR-like transcriptional regulators All alpha proteins; DNA/RNA-binding 3-helical bundle; "Winged helix" DNA-binding domain; MarR-like transcriptional regulators 6.19999980926514
Pfam IPR000488 EMBL-EBI Death domain 1.0E-20
Pfam IPR002110 EMBL-EBI Ankyrin 1.7
Pfam IPR002110 EMBL-EBI Ankyrin 4.4E-8
Pfam IPR002110 EMBL-EBI Ankyrin 1.5E-9
Pfam IPR002110 EMBL-EBI Ankyrin 4.7E-10
Pfam IPR002110 EMBL-EBI Ankyrin 5.2E-4
Pfam IPR002110 EMBL-EBI Ankyrin 0.048
Pfam IPR002110 EMBL-EBI Ankyrin 3.5E-11
Pfam IPR002110 EMBL-EBI Ankyrin 2.6E-7
Pfam IPR002110 EMBL-EBI Ankyrin 4.8E-9
Pfam IPR002110 EMBL-EBI Ankyrin 6.9E-6
Pfam IPR002110 EMBL-EBI Ankyrin 2.1E-9
Pfam IPR002110 EMBL-EBI Ankyrin 5.2E-10
Pfam IPR002110 EMBL-EBI Ankyrin 3.3E-10
Pfam IPR002110 EMBL-EBI Ankyrin 8.0E-11
Pfam IPR002110 EMBL-EBI Ankyrin 7.5E-10
Pfam IPR002110 EMBL-EBI Ankyrin 1.6E-7
Pfam IPR002110 EMBL-EBI Ankyrin 4.2E-11
Pfam IPR002110 EMBL-EBI Ankyrin 6.8E-7
Pfam IPR002110 EMBL-EBI Ankyrin 7.4E-11
Pfam IPR002110 EMBL-EBI Ankyrin 9.3E-8
Pfam IPR002110 EMBL-EBI Ankyrin 4.2E-7
Pfam IPR002110 EMBL-EBI Ankyrin 3.4E-10
Pfam IPR002110 EMBL-EBI Ankyrin 1.4E-9
Pfam IPR002110 EMBL-EBI Ankyrin 0.83
Pfam IPR000906 EMBL-EBI ZU5 domain 4.8E-62
Pfam IPR000488 EMBL-EBI Death domain 1.0E-20
Pfam IPR000906 EMBL-EBI ZU5 domain 1.4E-61
Pfam IPR000488 EMBL-EBI Death domain 1.0E-20
Pfam IPR000906 EMBL-EBI ZU5 domain 1.2E-49
Pfam IPR000488 EMBL-EBI Death domain 1.0E-20
Pfam IPR000906 EMBL-EBI ZU5 domain 4.8E-62
Sequence
Target Sequence 
>C. ELEGANS:172153_X_AT
aaccagttcaaccgacacatcatgttgaacaagcaaccactgtcgttcaggagccatctt
attcagcaccggttcatcatagtcctgttccagaagaacccgaaatggaagaagaagccc
cagtcgctgaaatgcgtaccgttgttcgaacggaacgacatgttcatgacagtgaagatg
gaccagttgtagaggaaagaactattacaacaacatacgaagatgacgtggcagtcaacg
agaacattgttgaccgcacggttccattgaacgaggatgaacagcaaaaatgggaagagc
tgaaccgtttggctgatgaatcctcaccatccccagctcaacgctcaacaattgtagctg
agagcacctccgagcaagttccagaagacgtcgagcaaagcgtagagtcggaaagccatc
gtgaagatgatggaacaattgtcacgacgacagtaaccactagtcacatcagcgaatctc
catcgggatcacctacaagacgatcagtggagcccgaagagcatcgtcata

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AACCAGTTCAACCGACACATCATGT 427 147 4880 Antisense
AAGCAACCACTGTCGTTCAGGAGCC 365 151 4910 Antisense
TCAGGAGCCATCTTATTCAGCACCG 452 623 4926 Antisense
CAGCACCGGTTCATCATAGTCCTGT 622 203 4943 Antisense
GAAGCCCCAGTCGCTGAAATGCGTA 66 343 4993 Antisense
TTGACCGCACGGTTCCATTGAACGA 143 697 5129 Antisense
ACCGTTTGGCTGATGAATCCTCACC 205 89 5183 Antisense
CACCTCCGAGCAAGTTCCAGAAGAC 437 201 5244 Antisense
ACTAGTCACATCAGCGAATCTCCAT 227 99 5338 Antisense
TCTCCATCGGGATCACCTACAAGAC 611 607 5356 Antisense
GTGGAGCCCGAAGAGCATCGTCATA 20 491 5386 Antisense
Sequence Source Affymetrix Proprietary Database