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[172147_x_at]

Description M116.5 /REP_DB=WormBase Gene ID /WP=CE25957 /GB=AAK71387.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_high1_10_10 > 87      6grp_total > 77      4grp_total > 23      6grp_high1_sub > In      6grp_total_sub > Increase      6grp_rule_based > S6      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 177
1 2 3 4 range
A 238.7
(2.1)
P 		152.8
(-3)
A 		184.5
(-1.1)
A 		254.6
(3)
P 		102
B 299.9
(3)
P 		151.4
(-2)
A 		123.3
(-3)
A 		217.3
(0.2)
P 		177
C 284.4
(3)
P 		165.3
(-3)
A 		171.6
(-2.7)
A 		254.7
(1.5)
P 		119
avr 274.3
(3) 		156.5
(-3) 		159.8
(-2.8) 		242.2
(1.4) 		118

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 385
1 2 3 4 5 6 range
A 167
(-2.3)
P 		189.2
(-1.7)
P 		143.7
(-3)
P 		248.7
(0)
P 		272.2
(0.7)
P 		353
(3)
P 		209
B 183
(-2.2)
P 		203
(-1.9)
P 		134.5
(-3)
P 		272.1
(-0.8)
P 		271.9
(-0.8)
P 		506.1
(3)
P 		372
C 121.6
(-3)
A 		227.5
(-1.2)
P 		186.9
(-1.9)
P 		170.5
(-2.2)
A 		248.2
(-0.8)
P 		471.5
(3)
P 		350
avr 157.2
(-3) 		206.6
(-1.9) 		155
(-3) 		230.4
(-1.4) 		264.1
(-0.7) 		443.5
(3) 		289
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 67
1 2 3 4 range
A 86.9
(3)
P 		33.2
(-3)
A 		48.2
(-1.3)
A 		37.3
(-2.5)
P 		54
B 95.7
(3)
P 		37.4
(-2.2)
A 		28.6
(-3)
A 		46.3
(-1.4)
P 		67
C 94.1
(3)
P 		47.5
(-2.5)
A 		59.8
(-1)
A 		42.9
(-3)
P 		51
avr 92.2
(3) 		39.4
(-3) 		45.5
(-2.3) 		42.2
(-2.7) 		53

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 158
1 2 3 4 5 6 range
A 30.8
(-3)
P 		67.3
(1)
P 		31.3
(-2.9)
P 		63.1
(0.5)
P 		82.1
(2.6)
P 		86
(3)
P 		55
B 63.7
(-1.8)
P 		67.6
(-1.7)
P 		32.7
(-3)
P 		72.1
(-1.5)
P 		110.5
(-0)
P 		188.7
(3)
P 		156
C 45.1
(-2.5)
A 		69.4
(-1.3)
P 		47.6
(-2.4)
P 		34.3
(-3)
A 		80.3
(-0.8)
P 		157.8
(3)
P 		124
avr 46.5
(-2.5) 		68.1
(-1.3) 		37.2
(-3) 		56.5
(-1.9) 		91
(0) 		144.2
(3) 		107
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 172147_x_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.9173
Sequence Type Exemplar sequence
Representative Public ID M116.5 NCBI
Target Description M116.5 /REP_DB=WormBase Gene ID /WP=CE25957 /GB=AAK71387.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_069031(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_069031 NCBI Caenorhabditis elegans M116.5 (M116.5) mRNA, complete cds. 11/11 A 
M116.5 ENSEMBL cdna:known chromosome:CEL140:IV:8414275:8420990:-1 gene:M116.5 11/11 A 
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_069025 8/11 Cross Hyb Matching Probes A 
SNAP00000025677 8/11 Cross Hyb Matching Probes None
GENEFINDER00000025699 8/11 Cross Hyb Matching Probes None
D2096.11 8/11 Cross Hyb Matching Probes A 
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:8414580-8420996(-) 99.23 100.0
chrIV:8389165-8393298(-) 78.87 79.64
Public Domain and Genome References
Gene Title
Ensembl M116.5
Entrez Gene 177642 Entrez gene
SwissProt Q965L0 EMBL-EBI
WormBase CE35345 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
7050 cell cycle arrest inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
3779 actin binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAK71387 Hypothetical protein M116.5 [Caenorhabditis elegans] ref|NP_501432.2| M116.5 [Caenorhabditis elegans] 0.0
blast NP_501426 D2096.11 [Caenorhabditis elegans] gb|AAA81498.4| Hypothetical protein D2096.11 [Caenorhabditis elegans] 0.0
blast CAD44516 VAB-10B protein [Caenorhabditis elegans] 1.0E-144
Protein Domains
Database ID Description E-Value
scop a.4.1.Centromere-binding All alpha proteins; DNA/RNA-binding 3-helical bundle; Homeodomain-like; Centromere-binding 2.20000004768372
Pfam IPR001715 EMBL-EBI Calponin-like actin-binding 2.9E-31
Pfam IPR001715 EMBL-EBI Calponin-like actin-binding 4.8E-31
Pfam IPR001715 EMBL-EBI Calponin-like actin-binding 4.5E-7
Pfam IPR001715 EMBL-EBI Calponin-like actin-binding 1.4E-11
Pfam IPR003108 EMBL-EBI Growth-arrest-specific protein 2 1.1E-23
Sequence
Target Sequence 
>C. ELEGANS:172147_X_AT
ttgtcatctttttcatgtctttccatgggtcgtaaattatttttttttggagatacccat
atcaagagaatggttcgtatccttcgatcaactgtgatggttcgtgttggcggtggatgg
gagccacttgaagagtcacttcaaaaacatgatccatgccgtacaaaaggacgtctcaat
atcaatatgttcccagaagctcgtccaattcatgctcttgacttgatgcaatcgttcact
aagaatcggcatgccaaacagatgcccattgccggaacgcctggaccaattatgaagatt
cgcgaaaaaactgagagaagtgttcccatgagcggaggactcggaggaaccgctggttac
acagttacaactgaatcgcctagaaaaacgcctcagcgcccgtttcgaattccacgtact
ccatcggacaagacccctggacgccaaagccgtgttggatgtgtctccaatttgaaaaac
tcgatcgtcgaaccgtcgtcaccttcccaatccgaatcgcgagcctcacctgaagtcgca
gaccgtcag

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TTGTCATCTTTTTCATGTCTTTCCA 60 709 2620 Antisense
GGTTCGTATCCTTCGATCAACTGTG 198 507 2691 Antisense
TGTGATGGTTCGTGTTGGCGGTGGA 706 553 2712 Antisense
TGGGAGCCACTTGAAGAGTCACTTC 177 553 2737 Antisense
AGAAGCTCGTCCAATTCATGCTCTT 656 73 2814 Antisense
GCATGCCAAACAGATGCCCATTGCC 19 309 2868 Antisense
GTGTTCCCATGAGCGGAGGACTCGG 460 485 2939 Antisense
GAGGACTCGGAGGAACCGCTGGTTA 200 405 2954 Antisense
CGCCTAGAAAAACGCCTCAGCGCCC 319 255 2996 Antisense
TTCCACGTACTCCATCGGACAAGAC 305 697 3029 Antisense
CTCACCTGAAGTCGCAGACCGTCAG 104 227 3144 Antisense
Sequence Source Affymetrix Proprietary Database