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[172144_x_at]

Description F14H12.4 /REP_DB=WormBase Gene ID /WP=CE07064 /CHR=X /FEA=Sanger Annotation /DEF=protein kinase (ST.LOUIS)
Cluster 6grp_highest_10_10 > 31      6grp_high1_10_10 > 03      4grp_highest_8_8 > 37      4grp_high_8_8 > 03      6grp_highest_nonorm > 31      6grp_total > 24      4grp_total > 06      6grp_highest_sub > De      6grp_high1_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 569
1 2 3 4 range
A 906.7
(0.2)
P 		689.5
(-3)
P 		740.6
(-2.2)
P 		1097.6
(3)
P 		408
B 865.4
(-0)
P 		621.3
(-3)
P 		803.8
(-0.8)
P 		1116
(3)
P 		495
C 1015.4
(1.1)
P 		647.9
(-3)
P 		957.1
(0.4)
P 		1190.7
(3)
P 		543
avr 929.2
(0.4) 		652.9
(-3) 		833.8
(-0.7) 		1134.8
(3) 		482

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 638
1 2 3 4 5 6 range
A 919.6
(0.1)
P 		1219.4
(3)
P 		1110.9
(1.9)
P 		846.4
(-0.6)
P 		601.5
(-3)
P 		659.1
(-2.4)
P 		618
B 1239.8
(3)
P 		1040.5
(0.8)
P 		873.4
(-1.1)
P 		702.2
(-3)
P 		1033.7
(0.7)
P 		764
(-2.3)
P 		538
C 1087.6
(3)
P 		995.8
(1.8)
P 		861.3
(0)
P 		730.8
(-1.7)
P 		628.8
(-3)
P 		646.3
(-2.8)
P 		459
avr 1082.3
(3) 		1085.2
(3) 		948.5
(0.9) 		759.8
(-1.9) 		754.7
(-2) 		689.8
(-3) 		395
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 186
1 2 3 4 range
A 330.1
(3)
P 		149.7
(-3)
P 		193.6
(-1.5)
P 		160.7
(-2.6)
P 		180
B 276
(3)
P 		153.5
(-3)
P 		186.3
(-1.4)
P 		237.8
(1.1)
P 		123
C 336.1
(3)
P 		186.3
(-3)
P 		333.6
(2.9)
P 		200.8
(-2.4)
P 		150
avr 314.1
(3) 		163.2
(-3) 		237.8
(-0) 		199.8
(-1.5) 		151

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 287
1 2 3 4 5 6 range
A 169.5
(-2.8)
P 		433.7
(3)
P 		241.9
(-1.2)
P 		214.7
(-1.8)
P 		181.5
(-2.5)
P 		160.5
(-3)
P 		273
B 431.4
(3)
P 		346.3
(0.9)
P 		212.1
(-2.4)
P 		186
(-3)
P 		420
(2.7)
P 		284.9
(-0.6)
P 		245
C 403.1
(3)
P 		303.8
(0.7)
P 		219.2
(-1.3)
P 		147
(-3)
P 		203.5
(-1.7)
P 		216.4
(-1.4)
P 		256
avr 334.7
(2.1) 		361.3
(3) 		224.4
(-1.6) 		182.6
(-3) 		268.3
(-0.1) 		220.6
(-1.7) 		179
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 172144_x_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.16481
Sequence Type Exemplar sequence
Representative Public ID F14H12.4 NCBI
Target Description F14H12.4 /REP_DB=WormBase Gene ID /WP=CE07064 /CHR=X /FEA=Sanger Annotation /DEF=protein kinase (ST.LOUIS)
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_076342(11), NM_001029404(10)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_076342 NCBI Caenorhabditis elegans F14H12.4a (F14H12.4) mRNA, complete cds. 11/11 A 
NM_001029404 NCBI Caenorhabditis elegans F14H12.4b (F14H12.4) mRNA, complete cds. 10/11 None
GENEFINDER00000002247 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:4339618:4343557:1 11/11 None
SNAP00000002231 ENSEMBL cdna:SNAP chromosome:CEL140:X:4341821:4342472:1 9/11 None
F14H12.4b ENSEMBL cdna:known chromosome:CEL140:X:4339534:4343494:1 gene:F14H12.4 10/11 A 
F14H12.4a ENSEMBL cdna:known chromosome:CEL140:X:4339534:4343610:1 gene:F14H12.4 11/11 A 
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_076340 7/11 Cross Hyb Matching Probes A 
NM_076341 7/11 Cross Hyb Matching Probes A 
SNAP00000002228 3/11 Cross Hyb Matching Probes None
GENEFINDER00000002241 3/11 Cross Hyb Matching Probes None
C24A8.4a 7/11 Cross Hyb Matching Probes A 
C24A8.4b 7/11 Cross Hyb Matching Probes A 
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:4339534-4343487(+) 97.26 100.0
chrX:4333206-4336093(-) 80.6 83.33
Public Domain and Genome References
Ensembl F14H12.4
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAK68326 Hypothetical protein F14H12.4a [Caenorhabditis elegans] ref|NP_508743.3| F14H12.4a [Caenorhabditis elegans] 0.0
blast AAK68327 Hypothetical protein F14H12.4b [Caenorhabditis elegans] ref|NP_001024575.1| F14H12.4b [Caenorhabditis elegans] 0.0
blast AAF75788 STE20-like kinase MST [Caenorhabditis elegans] 0.0
Protein Families
Method ID Description E-Value
ec A2S7_HUMAN (Q96Q40) Serine/threonine-protein kinase ALS2CR7 (EC 2.7.1.37) (Amyotrophic lateral sclerosis 2 chromosomal region candidate gene protein 7) 1.0E-64
ec A2S7_HUMAN (Q96Q40) Serine/threonine-protein kinase ALS2CR7 (EC 2.7.1.37) (Amyotrophic lateral sclerosis 2 chromosomal region candidate gene protein 7) 3.0E-65
hanks 2.1.17 CaMK Group; CaMK I Regulated by Ca2+/CaM and close relatives; TWITCH 1.0E-71
hanks 2.1.17 CaMK Group; CaMK I Regulated by Ca2+/CaM and close relatives; TWITCH 6.0E-72
Protein Domains
Database ID Description E-Value
Pfam IPR000719 EMBL-EBI Protein kinase 8.5E-93
Pfam IPR000719 EMBL-EBI Protein kinase 8.5E-93
Sequence
Target Sequence 
>C. ELEGANS:172144_X_AT
agcaccaatggcagcaagcagcggaaatgatgcaacgttgctaagcgagggaatgtccac
tatgattgacggtggagagtctacgttagttcaacacaaagacaactatgtcactgctca
aagtcttcgaagtcaaatggaaagcttgagaattggtggagaaattcctaaatcggcata
tagctcgtcgaaaaatggcaactcaccgcgcgttcaaccaccgggtcacaccgcaagcgc
ttcagacccttcaaaaaaccaaccgtttgctcaagatggaaccggtccaaactttcaatt
gggcaccagcgagagcagttacaaagatgctagttataatatgatgaacacggaagccga
atacgaaaatagatttcaacgggcagtggtcgatggagatttcgaatttttacgaaacat
cacgctagacgaactg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AGCACCAATGGCAGCAAGCAGCGGA 13 77 1026 Antisense
GGGAATGTCCACTATGATTGACGGT 69 495 1074 Antisense
GGAGAGTCTACGTTAGTTCAACACA 285 517 1099 Antisense
GTCACTGCTCAAAGTCTTCGAAGTC 216 465 1135 Antisense
TCCTAAATCGGCATATAGCTCGTCG 240 593 1191 Antisense
GTCGAAAAATGGCAACTCACCGCGC 266 475 1212 Antisense
AACCGTTTGCTCAAGATGGAACCGG 492 145 1286 Antisense
TGGAACCGGTCCAAACTTTCAATTG 105 545 1302 Antisense
ATTGGGCACCAGCGAGAGCAGTTAC 227 3 1323 Antisense
GATTTCAACGGGCAGTGGTCGATGG 326 427 1397 Antisense
ACGAAACATCACGCTAGACGAACTG 643 89 1437 Antisense
Sequence Source Affymetrix Proprietary Database