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[172138_x_at]

Description F52C6.12 /REP_DB=WormBase Gene ID /WP=CE10824 /GB=AAB94978.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_total > 29      4grp_total > 08      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1055
1 2 3 4 range
A 1402.3
(-2.5)
A 		1315.1
(-3)
A 		2300
(3)
A 		1923.2
(0.7)
A 		985
B 1715.4
(-0.3)
M 		1410.2
(-2.3)
A 		1299.7
(-3)
A 		2232.7
(3)
A 		933
C 1245.1
(-3)
A 		1541
(-0.2)
A 		1539.3
(-0.2)
A 		1879.2
(3)
M 		634
avr 1454.3
(-2.7) 		1422.1
(-3) 		1713
(-0) 		2011.7
(3) 		590

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 925
1 2 3 4 5 6 range
A 1473.7
(1.8)
A 		1128.3
(-2.3)
M 		1069
(-3)
A 		1202.5
(-1.4)
P 		1571.6
(3)
P 		1517.5
(2.4)
P 		503
B 1266.6
(0.4)
M 		1538.1
(3)
A 		921.8
(-3)
M 		1329.8
(1)
P 		1521.9
(2.8)
A 		1305.1
(0.7)
A 		616
C 1846.7
(3)
A 		1269.9
(-1.3)
A 		1511.1
(0.5)
P 		1384.8
(-0.4)
P 		1360.4
(-0.6)
A 		1037.1
(-3)
M 		810
avr 1529
(3) 		1312.1
(-0.6) 		1167.3
(-3) 		1305.7
(-0.7) 		1484.6
(2.3) 		1286.6
(-1) 		362
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 320
1 2 3 4 range
A 510.6
(1.3)
A 		285.5
(-2.9)
A 		601.3
(3)
A 		281.5
(-3)
A 		320
B 547
(3)
M 		348.3
(-1.9)
A 		301.2
(-3)
A 		475.8
(1.3)
A 		246
C 412.2
(-0.4)
A 		443
(0.4)
A 		536.5
(3)
A 		316.9
(-3)
M 		220
avr 489.9
(3) 		358.9
(-3) 		479.7
(2.5) 		358.1
(-3) 		132

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 461
1 2 3 4 5 6 range
A 271.7
(-2)
A 		401.3
(1.2)
M 		232.8
(-3)
A 		305
(-1.2)
P 		474.2
(3)
P 		369.5
(0.4)
P 		241
B 440.7
(0.3)
M 		512
(1.4)
A 		223.8
(-3)
M 		352.2
(-1)
P 		618.3
(3)
A 		486.7
(1)
A 		395
C 684.5
(3)
A 		387.5
(-1.4)
A 		384.6
(-1.4)
P 		278.5
(-3)
P 		440.2
(-0.6)
A 		347.2
(-2)
M 		406
avr 465.6
(1.8) 		433.6
(1) 		280.4
(-3) 		311.9
(-2.2) 		510.9
(3) 		401.1
(0.1) 		231
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 172138_x_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.5207
Sequence Type Exemplar sequence
Representative Public ID F52C6.12 NCBI
Target Description F52C6.12 /REP_DB=WormBase Gene ID /WP=CE10824 /GB=AAB94978.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_061729(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_061729 NCBI Caenorhabditis elegans F52C6.12 (F52C6.12) mRNA, complete cds. 11/11 None
GENEFINDER00000005738 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:1929366:1930084:-1 11/11 None
F52C6.12.1 ENSEMBL cdna:known chromosome:CEL140:II:1929367:1929954:-1 gene:F52C6.12 11/11 None
F52C6.12.2 ENSEMBL cdna:known chromosome:CEL140:II:1929431:1929920:-1 gene:F52C6.12 9/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_069664 4/11 Cross Hyb Matching Probes A 
SNAP00000005728 7/11 Cross Hyb Matching Probes None
SNAP00000010381 5/11 Cross Hyb Matching Probes None
GENEFINDER00000010391 5/11 Cross Hyb Matching Probes None
M7.1 6/11 Cross Hyb Matching Probes A 
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:1929407-1929919(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F52C6.12
Entrez Gene 186094 Entrez gene
SwissProt Q8MYD4 EMBL-EBI
WormBase CE35975 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6464 protein modification inferred from electronic annotation QuickGO AmiGO
6512 ubiquitin cycle inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4840 ubiquitin conjugating enzyme activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAB94978 Hypothetical protein F52C6.12 [Caenorhabditis elegans] ref|NP_494130.2| F52C6.12 [Caenorhabditis elegans] 5.0E-40
blast CAA92745 Hypothetical protein M7.1 [Caenorhabditis elegans] ref|NP_502065.1| LEThal family member (let-70) [Caenorhabditis elegans] gb|AAB25489.2| ubiquitin-conjugating enzyme [Caenorhabditis elegans] sp|P35129|UBC2_CAEEL Ubiquitin-conjugating enzyme E2 2 (Ubiquitin-protein ligase 2) (Ubiquitin carrier protein 2) emb|CAE61994.1| Hypothetical protein CBG06002 [Caenorhabditis briggsae] 2.0E-23
blast 1Z2U Chain , The 1.1a Crystallographic Structure Of Ubiquitin- Conjugating Enzyme (Ubc-2) From Caenorhabditis Elegans: Functional And Evolutionary Significance 2.0E-23
Protein Domains
Database ID Description E-Value
Pfam IPR000608 EMBL-EBI Ubiquitin-conjugating enzymes 2.0E-17
Sequence
Target Sequence 
>C. ELEGANS:172138_X_AT
cagaaagaactccatgatctcggccgtgatccacccgcacaatgctccactggatcagtt
ggtgatgatttgtaccattggcaagctacgattatgggcccaccagagtctccctatcag
ggagttctgctttcgatctgctcgctgctctgtgatccaaatccggatgaacaacgatac
cgccgctc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CAGAAAGAACTCCATGATCTCGGCC 454 205 31 Antisense
CCGCACAATGCTCCACTGGATCAGT 397 263 65 Antisense
TGCTCCACTGGATCAGTTGGTGATG 33 583 73 Antisense
GTGATGATTTGTACCATTGGCAAGC 95 485 92 Antisense
GGCAAGCTACGATTATGGGCCCACC 376 535 110 Antisense
TACGATTATGGGCCCACCAGAGTCT 639 645 117 Antisense
CCACCAGAGTCTCCCTATCAGGGAG 622 273 130 Antisense
TCCCTATCAGGGAGTTCTGCTTTCG 236 597 141 Antisense
TCAGGGAGTTCTGCTTTCGATCTGC 629 615 147 Antisense
GCTGCTCTGTGATCCAAATCCGGAT 223 297 174 Antisense
CGGATGAACAACGATACCGCCGCTC 532 243 194 Antisense
Sequence Source Affymetrix Proprietary Database