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[172124_x_at]

Description Y65B4A.D_rc /REP_DB=WormBase Gene ID /WP=CE22730 /CHR=1 /FEA=Sanger Annotation /REVCOMP /DEF=(ST.LOUIS)
Cluster 6grp_highest_10_10 > 37      6grp_high1_10_10 > 73      4grp_highest_8_8 > 23      4grp_high_8_8 > 13      6grp_highest_nonorm > 81      6grp_total > 60      4grp_total > 10      6grp_highest_sub > 03_04      6grp_high1_sub > 03_04      6grp_total_sub > Hat      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 766
1 2 3 4 range
A 242.1
(-2.9)
P 		286.8
(-2.5)
P 		235.9
(-3)
P 		791.3
(3)
P 		555
B 282
(-2.1)
P 		315.8
(-1.7)
P 		195.5
(-3)
P 		754.6
(3)
P 		559
C 256.4
(-2.6)
P 		205
(-3)
P 		253.5
(-2.6)
P 		961
(3)
P 		756
avr 260.2
(-2.7) 		269.2
(-2.6) 		228.3
(-3) 		835.6
(3) 		607

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 196
1 2 3 4 5 6 range
A 274.1
(-2.4)
P 		290.5
(-1.7)
P 		405
(2.8)
P 		411
(3)
P 		257.7
(-3)
P 		292.4
(-1.6)
P 		153
B 282.3
(-2.8)
P 		281.6
(-2.8)
P 		330.9
(-0.8)
P 		350.9
(-0)
P 		276.3
(-3)
P 		427.2
(3)
P 		151
C 300.7
(0.9)
P 		278
(-0.4)
P 		310.7
(1.4)
P 		338.9
(3)
P 		284.6
(-0)
P 		231.6
(-3)
P 		107
avr 285.7
(-2.2) 		283.4
(-2.3) 		348.9
(1.9) 		366.9
(3) 		272.9
(-3) 		317.1
(-0.2) 		94
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 117
1 2 3 4 range
A 88.2
(-0.1)
P 		62.3
(-2.9)
P 		61.7
(-3)
P 		115.8
(3)
P 		54
B 89.9
(-0.7)
P 		78
(-1.3)
P 		45.3
(-3)
P 		160.8
(3)
P 		116
C 84.9
(-1.5)
P 		58.9
(-3)
P 		88.3
(-1.3)
P 		162
(3)
P 		103
avr 87.7
(-1.3) 		66.4
(-2.9) 		65.1
(-3) 		146.2
(3) 		81

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 109
1 2 3 4 5 6 range
A 50.5
(-3)
P 		103.3
(2.9)
P 		88.2
(1.2)
P 		104.2
(3)
P 		77.8
(0.1)
P 		71.2
(-0.7)
P 		54
B 98.2
(-1.6)
P 		93.7
(-2)
P 		80.4
(-3)
P 		93
(-2)
P 		112.3
(-0.6)
P 		159.3
(3)
P 		79
C 111.5
(3)
P 		84.8
(-0.7)
P 		79.1
(-1.5)
P 		68.2
(-3)
P 		92.1
(0.3)
P 		77.5
(-1.7)
P 		43
avr 86.7
(-1.8) 		93.9
(0.4) 		82.6
(-3) 		88.5
(-1.2) 		94.1
(0.4) 		102.7
(3) 		20
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 172124_x_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.28179
Sequence Type Exemplar sequence
Representative Public ID Y65B4A.D NCBI
Target Description Y65B4A.D_rc /REP_DB=WormBase Gene ID /WP=CE22730 /CHR=1 /FEA=Sanger Annotation /REVCOMP /DEF=(ST.LOUIS)
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_058361(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_058361 NCBI Caenorhabditis elegans Y65B4A.3 (Y65B4A.3) mRNA, complete cds. 11/11 A 
SNAP00000032533 ENSEMBL cdna:SNAP chromosome:CEL140:I:619311:637714:1 11/11 None
GENEFINDER00000032549 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:636939:637810:1 11/11 None
Y65B4A.3 ENSEMBL cdna:known chromosome:CEL140:I:636865:638275:1 gene:Y65B4A.3 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:637486-637675(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl Y65B4A.3
Entrez Gene 171654 Entrez gene
SwissProt Q9BL60 EMBL-EBI
WormBase CE22731 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:247302_AT SNF7 family protein at
CANINE_2:CFA.21067.1.S1_AT similar to chromatin modifying protein 6 cfa
CANINE_2:CFAAFFX.9501.1.S1_S_AT similar to chromatin modifying protein 6 cfa
DROSOPHILA_2:1635121_A_AT dm
DROSGENOME1:147687_AT dm
HU35KSUBA:RC_AA427638_AT chromatin modifying protein 6 hs
HG-U133A:218743_AT chromatin modifying protein 6 hs
HG-U133_PLUS_2:218743_AT chromatin modifying protein 6 hs
HG-U133A_2:218743_AT chromatin modifying protein 6 hs
U133_X3P:G13375782_3P_AT chromatin modifying protein 6 hs
HG-U95B:56326_AT chromatin modifying protein 6 hs
MG-U74AV2:96097_AT chromatin modifying protein 6 mm
MOE430A:1433684_AT chromatin modifying protein 6 mm
MOUSE430A_2:1433684_AT chromatin modifying protein 6 mm
MOUSE430_2:1433684_AT chromatin modifying protein 6 mm
MU19KSUBB:TC32060_AT PREDICTED: hypothetical protein XP_126613 [Mus musculus], mRNA sequence mm
MU19KSUBB:TC34092_AT PREDICTED: hypothetical protein XP_126613 [Mus musculus], mRNA sequence mm
RG-U34B:RC_AA944274_AT similar to hypothetical protein FLJ11749 rn
RAE230A:1388510_AT similar to hypothetical protein FLJ11749 rn
RAT230_2:1388510_AT similar to hypothetical protein FLJ11749 rn
YEAST_2:1773106_AT Myristoylated subunit of ESCRTIII, the endosomal sorting complex required for transport of transmembrane proteins into the multivesicular body pathway to the lysosomal/vacuolar lumen; cytoplasmic protein recruited to endosomal membranes Sc
Protein Similarities
Method ID Description E-Value
blast AAF99978 Hypothetical protein F56A6.1a [Caenorhabditis elegans] ref|NP_490758.1| F56A6.1a [Caenorhabditis elegans] 0.0
blast AAO38667 Paz/piwi domain-containing protein 1, isoform c [Caenorhabditis elegans] ref|NP_491323.2| PAZ/PIWI domain-containing family member (ppw-1) [Caenorhabditis elegans] 0.0
blast AAB52946 Paz/piwi domain-containing protein 1, isoform a [Caenorhabditis elegans] ref|NP_740835.1| PAZ/PIWI domain-containing family member (ppw-1) [Caenorhabditis elegans] 0.0
blast AAK29955 Hypothetical protein Y65B4A.3 [Caenorhabditis elegans] ref|NP_490762.1| Y65B4A.3 [Caenorhabditis elegans] 3.0E-83
blast CAE61309 Hypothetical protein CBG05144 [Caenorhabditis briggsae] 6.0E-71
blast CAG03061 unnamed protein product [Tetraodon nigroviridis] 6.0E-34
Protein Domains
Database ID Description E-Value
Pfam IPR005024 EMBL-EBI Snf7 1.2E-59
Pfam IPR005024 EMBL-EBI Snf7 8.6E-56
Pfam IPR003100 EMBL-EBI Argonaute and Dicer protein, PAZ 2.9E-46
Pfam IPR003165 EMBL-EBI Stem cell self-renewal protein Piwi 1.0E-126
Sequence
Target Sequence 
>C. ELEGANS:172124_X_AT
aatcgacaggattatggaagaaaccaaagaggcggcggaatatcaggaagaaatctcgaa
tatgctgtccggccagctttccaacacggatgtctcggacgttgagaaggaattggagga
tctgttggcggcggaat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AATCGACAGGATTATGGAAGAAACC 328 151 30 Antisense
GAAGAAACCAAAGAGGCGGCGGAAT 422 331 46 Antisense
GAGGCGGCGGAATATCAGGAAGAAA 362 405 58 Antisense
GAAATCTCGAATATGCTGTCCGGCC 346 363 79 Antisense
CGAATATGCTGTCCGGCCAGCTTTC 361 253 86 Antisense
CCAGCTTTCCAACACGGATGTCTCG 243 273 102 Antisense
TTCCAACACGGATGTCTCGGACGTT 78 697 108 Antisense
AACACGGATGTCTCGGACGTTGAGA 209 135 112 Antisense
TCGGACGTTGAGAAGGAATTGGAGG 166 603 124 Antisense
GGAATTGGAGGATCTGTTGGCGGCG 134 533 138 Antisense
TTGGAGGATCTGTTGGCGGCGGAAT 490 703 142 Antisense
Sequence Source Affymetrix Proprietary Database