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[172100_x_at]

Description C36E6.B /REP_DB=WormBase Gene ID /WP=CE20542 /CHR=X /FEA=Sanger Annotation /DEF=locus:mlc-1 (ST.LOUIS)
Cluster 6grp_highest_10_10 > 05      6grp_high1_10_10 > 50      4grp_highest_8_8 > 01      4grp_high_8_8 > 53      6grp_highest_nonorm > 09      6grp_total > 41      4grp_total > 58      6grp_highest_sub > 03_04      6grp_cgc > 83      6grp_high1_sub > 03_04      6grp_cgc_sub > 03      6grp_total_sub > Hat      6grp_rule_based > S3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 6038
1 2 3 4 range
A 28002.7
(-1.8)
P 		30376
(1.4)
P 		31576.7
(3)
P 		27136.4
(-3)
P 		4440
B 29476.2
(1.3)
P 		28821.3
(0)
P 		30353.5
(3)
P 		27272.6
(-3)
P 		3081
C 26608.3
(-1.5)
P 		29782.7
(3)
P 		25538.4
(-3)
P 		26494.9
(-1.6)
P 		4244
avr 28029.1
(-0.6) 		29660
(3) 		29156.2
(1.9) 		26968
(-3) 		2692

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 17524
1 2 3 4 5 6 range
A 31700.3
(2.2)
P 		29052.5
(0.4)
P 		31203.4
(1.8)
P 		32941.8
(3)
P 		25995.1
(-1.6)
P 		23927.6
(-3)
P 		9014
B 27319.7
(1.5)
P 		27790.4
(1.7)
P 		30501.1
(3)
P 		28760.6
(2.2)
P 		23263.9
(-0.5)
P 		18042.3
(-3)
P 		12459
C 26298.4
(-0.3)
P 		27905.8
(0.3)
P 		35566.5
(3)
P 		31655.5
(1.6)
P 		28803.8
(0.6)
P 		18531.5
(-3)
P 		17035
avr 28439.5
(1) 		28249.6
(1) 		32423.7
(3) 		31119.3
(2.4) 		26020.9
(-0.1) 		20167.1
(-3) 		12257
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 6224
1 2 3 4 range
A 10195.8
(3)
P 		6593.8
(-0.5)
P 		8254.8
(1.1)
P 		3972.2
(-3)
P 		6224
B 9399.7
(3)
P 		7118.2
(-0.8)
P 		7033.6
(-1)
P 		5811.8
(-3)
P 		3588
C 8808.1
(2.9)
P 		8562.7
(2.5)
P 		8900.9
(3)
P 		4467.4
(-3)
P 		4434
avr 9467.9
(3) 		7424.9
(0.4) 		8063.1
(1.2) 		4750.5
(-3) 		4717

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 4507
1 2 3 4 5 6 range
A 5843.7
(-3)
P 		10332.5
(3)
P 		6795.4
(-1.7)
P 		8354.8
(0.4)
P 		7844.2
(-0.3)
P 		5825.6
(-3)
P 		4507
B 9505.9
(3)
P 		9250.2
(2.4)
P 		7406.6
(-1.5)
P 		7617.7
(-1.1)
P 		9451.8
(2.9)
P 		6727.9
(-3)
P 		2778
C 9747.7
(3)
P 		8514.9
(0.9)
P 		9052.3
(1.8)
P 		6366
(-2.7)
P 		9320.5
(2.3)
P 		6203.6
(-3)
P 		3544
avr 8365.8
(1.1) 		9365.9
(3) 		7751.4
(-0.1) 		7446.2
(-0.7) 		8872.2
(2) 		6252.4
(-3) 		3114
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 172100_x_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.19568
Sequence Type Exemplar sequence
Representative Public ID C36E6.B NCBI
Target Description C36E6.B /REP_DB=WormBase Gene ID /WP=CE20542 /CHR=X /FEA=Sanger Annotation /DEF=locus:mlc-1 (ST.LOUIS)
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_078427(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_078427 NCBI Caenorhabditis elegans Myosin Light Chain family member (mlc-2) (mlc-2) mRNA, complete cds. 11/11 A 
SNAP00000036942 ENSEMBL cdna:SNAP chromosome:CEL140:X:17455812:17456478:-1 11/11 None
GENEFINDER00000036960 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:17455812:17456478:-1 11/11 None
C36E6.5.3 ENSEMBL cdna:known chromosome:CEL140:X:17455618:17456505:-1 gene:C36E6.5 11/11 None
C36E6.5.2 ENSEMBL cdna:known chromosome:CEL140:X:17455638:17456505:-1 gene:C36E6.5 11/11 A 
C36E6.5.1 ENSEMBL cdna:known chromosome:CEL140:X:17455638:17456497:-1 gene:C36E6.5 11/11 A 
C36E6.5.4 ENSEMBL cdna:known chromosome:CEL140:X:17455716:17456502:-1 gene:C36E6.5 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_078428 3/11 Cross Hyb Matching Probes A B 
SNAP00000036943 3/11 Cross Hyb Matching Probes None
GENEFINDER00000036964 3/11 Cross Hyb Matching Probes None
C36E6.3 3/11 Cross Hyb Matching Probes A B 
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:17459400-17460066(+) 94.93 94.93
chrX:17455810-17456477(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol mlc-2
Ensembl C36E6.5
Entrez Gene 181775 Entrez gene
SwissProt P19626 EMBL-EBI
WormBase CE20542 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE:1590988_S_AT similar to Myosin regulatory light chain 2, skeletal muscle isoform (G2) (DTNB) (MLC-2) cfa
CANINE:1591371_S_AT similar to Myosin regulatory light chain 2, skeletal muscle isoform (G2) (DTNB) (MLC-2) cfa
CANINE_2:CFA.1238.8.S1_S_AT similar to Myosin regulatory light chain 2, skeletal muscle isoform (G2) (DTNB) (MLC-2) cfa
HG-U133_PLUS_2:205163_AT fast skeletal myosin light chain 2 hs
HG-U133A:205163_AT fast skeletal myosin light chain 2 hs
HG-FOCUS:205163_AT fast skeletal myosin light chain 2 hs
HG-U133A_2:205163_AT fast skeletal myosin light chain 2 hs
HUGENEFL:M21812_AT fast skeletal myosin light chain 2 hs
U133_X3P:G7019426_3P_AT fast skeletal myosin light chain 2 hs
HG-U95AV2:34881_AT fast skeletal myosin light chain 2 hs
MU11KSUBA:U77943_S_AT myosin light chain, phosphorylatable, fast skeletal muscle mm
MU11KSUBB:MSA.17147.0_F_AT myosin light chain, phosphorylatable, fast skeletal muscle mm
MU11KSUBB:MSA.3233.0_F_AT myosin light chain, phosphorylatable, fast skeletal muscle mm
MU11KSUBB:MSA.24240.0_S_AT myosin light chain, phosphorylatable, fast skeletal muscle mm
MG-U74AV2:92881_AT myosin light chain, phosphorylatable, fast skeletal muscle mm
MG-U74AV2:162101_F_AT myosin light chain, phosphorylatable, fast skeletal muscle mm
MG-U74CV2:170347_F_AT myosin light chain, phosphorylatable, fast skeletal muscle mm
MOE430A:1448371_AT myosin light chain, phosphorylatable, fast skeletal muscle mm
MOUSE430A_2:1448371_AT myosin light chain, phosphorylatable, fast skeletal muscle mm
MOUSE430_2:1448371_AT myosin light chain, phosphorylatable, fast skeletal muscle mm
MU11KSUBB:MSA.30406.0_F_AT Myosin light chain, phosphorylatable, fast skeletal muscle (Mylpf), mRNA mm
MU11KSUBB:MSA.6060.0_AT Myosin light chain, phosphorylatable, fast skeletal muscle (Mylpf), mRNA mm
MU19KSUBA:TC22501_AT Myosin light chain, phosphorylatable, fast skeletal muscle (Mylpf), mRNA mm
MU19KSUBA:TC22502_F_AT Myosin light chain, phosphorylatable, fast skeletal muscle (Mylpf), mRNA mm
RG-U34A:X00975_AT myosin, light polypeptide 2 rn
RG-U34A:X00975_G_AT myosin, light polypeptide 2 rn
RAE230A:1387787_AT myosin, light polypeptide 2 rn
RAT230_2:1387787_AT myosin, light polypeptide 2 rn
RG-U34B:RC_AA998118_AT Myosin, light polypeptide 2 rn
RG-U34C:RC_AI230218_AT Myosin, light polypeptide 2 rn
Gene Ontology
GO Molecular Function (view graph)
ID Description Evidence Links
5509 calcium ion binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAK09069 Myosin light chain protein 2 [Caenorhabditis elegans] ref|NP_510828.1| Myosin Light Chain family member (mlc-2) [Caenorhabditis elegans] sp|P19626|MLR2_CAEEL Myosin regulatory light chain 2 gb|AAA28114.1| myosin light chain 2.0E-81
blast AAK09070 Myosin light chain protein 1 [Caenorhabditis elegans] ref|NP_510829.2| Myosin Light Chain family member (mlc-1) [Caenorhabditis elegans] sp|P19625|MLR1_CAEEL Myosin regulatory light chain 1 gb|AAA28113.1| myosin light chain 2.0E-81
Protein Domains
Database ID Description E-Value
Pfam IPR002048 EMBL-EBI Calcium-binding EF-hand 1.7E-6
Pfam IPR002048 EMBL-EBI Calcium-binding EF-hand 1.3
Sequence
Target Sequence 
>C. ELEGANS:172100_X_AT
agaagtcctcaaagaagcgctcgggatccgaggccgcccaattcgaccagaagaccatcc
aggagttcaaggaggccttcggaatcatggatcagaacaaggacggaatcatcgacaagt
ctgacctcaaggatctctacgcatccatgggacaaatcgccccagacagccagatcgacg
ccatgatcaaggaggcatccggaccaatcaacttcaccgtcttcctcacccttttcgggg
agagattgacaggaactgacccagaggccaccattgttggagcttttgctatgttcgata
agaaggactgcggaaagatcaaggaggacgaccttatcaagatccttcaaaacaagagag
gagagccattagacgaggatgaggtcaaggctatgtacaagggaaagccaccaatcgagg
gaggagaggttgactacaaggcattcgctcacctc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AGAAGTCCTCAAAGAAGCGCTCGGG 59 75 32 Antisense
CGCCCAATTCGACCAGAAGACCATC 358 255 66 Antisense
GGAGGCCTTCGGAATCATGGATCAG 258 515 102 Antisense
GTCTGACCTCAAGGATCTCTACGCA 535 471 150 Antisense
GCATCCATGGGACAAATCGCCCCAG 90 301 172 Antisense
GACAGCCAGATCGACGCCATGATCA 5 369 196 Antisense
CATCCGGACCAATCAACTTCACCGT 645 211 227 Antisense
TTCCTCACCCTTTTCGGGGAGAGAT 59 699 253 Antisense
TGACCCAGAGGCCACCATTGTTGGA 52 571 288 Antisense
GGACGACCTTATCAAGATCCTTCAA 75 525 357 Antisense
TGACTACAAGGCATTCGCTCACCTC 440 571 462 Antisense
Sequence Source Affymetrix Proprietary Database