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[172074_x_at]

Description Y69A2AR.Z /REP_DB=WormBase Gene ID /WP=CE22804 /CHR=4 /FEA=Sanger Annotation /DEF=(ST.LOUIS)
Cluster 6grp_total > 85      4grp_total > 09      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 64
1 2 3 4 range
A 5.4
(-3)
A 		18.5
(-1.4)
A 		19.7
(-1.3)
A 		54.9
(3)
A 		50
B 9.7
(-0.7)
A 		3.1
(-3)
A 		6.4
(-1.8)
A 		20.1
(3)
A 		17
C 4.2
(-2.8)
A 		6.4
(-2.6)
A 		2.3
(-3)
A 		66.6
(3)
A 		64
avr 6.4
(-3) 		9.3
(-2.6) 		9.5
(-2.5) 		47.2
(3) 		41

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 44
1 2 3 4 5 6 range
A 4.8
(-3)
A 		26.9
(0.6)
A 		6.8
(-2.7)
A 		13.6
(-1.6)
A 		31.9
(1.5)
A 		41.3
(3)
A 		37
B 3.3
(-3)
A 		4
(-2.9)
A 		9.7
(-2.1)
A 		45.7
(3)
A 		25.7
(0.2)
A 		36.7
(1.7)
A 		42
C 1.6
(-3)
A 		7
(-2)
A 		2.8
(-2.8)
A 		34.1
(3)
A 		3.5
(-2.7)
A 		34.3
(3)
A 		33
avr 3.2
(-3) 		12.6
(-1.4) 		6.4
(-2.4) 		31.1
(1.9) 		20.4
(0) 		37.4
(3) 		34
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 10
1 2 3 4 range
A 2
(-3)
A 		4
(-1)
A 		5.2
(0.2)
A 		8
(3)
A 		6
B 3.1
(0.9)
A 		0.8
(-3)
A 		1.5
(-1.8)
A 		4.3
(3)
A 		4
C 1.4
(-2.7)
A 		1.8
(-2.4)
A 		0.8
(-3)
A 		11.2
(3)
A 		10
avr 2.2
(-3) 		2.2
(-3) 		2.5
(-2.7) 		7.8
(3) 		6

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 13
1 2 3 4 5 6 range
A 0.9
(-3)
A 		9.6
(2.7)
A 		1.5
(-2.6)
A 		3.5
(-1.3)
A 		9.6
(2.7)
A 		10.1
(3)
A 		9
B 1.1
(-3)
A 		1.3
(-2.9)
A 		2.4
(-2.4)
A 		12.1
(2.2)
A 		10.4
(1.4)
A 		13.7
(3)
A 		13
C 0.6
(-3)
A 		2.1
(-2.2)
A 		0.7
(-2.9)
A 		6.9
(0.5)
A 		1.1
(-2.7)
A 		11.5
(3)
A 		11
avr 0.9
(-3) 		4.3
(-1.1) 		1.5
(-2.7) 		7.5
(0.6) 		7
(0.4) 		11.8
(3) 		11
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 172074_x_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.19369
Sequence Type Exemplar sequence
Representative Public ID Y69A2AR.Z NCBI
Target Description Y69A2AR.Z /REP_DB=WormBase Gene ID /WP=CE22804 /CHR=4 /FEA=Sanger Annotation /DEF=(ST.LOUIS)
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_067828(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_067828 NCBI Caenorhabditis elegans Y69A2AR.8 (Y69A2AR.8) mRNA, complete cds. 11/11 None
SNAP00000018816 ENSEMBL cdna:SNAP chromosome:CEL140:IV:2585099:2601395:1 9/11 None
GENEFINDER00000018837 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:2584774:2596813:1 9/11 None
Y69A2AR.8 ENSEMBL cdna:known chromosome:CEL140:IV:2596331:2596783:1 gene:Y69A2AR.8 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_067855 3/11 Cross Hyb Matching Probes A 
SNAP00000036480 3/11 Cross Hyb Matching Probes None
GENEFINDER00000036610 3/11 Cross Hyb Matching Probes None
Y54G2A.13 3/11 Cross Hyb Matching Probes A 
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:2596336-2596789(+) 52.89 100.0
Public Domain and Genome References
Gene Title
Ensembl Y69A2AR.8
Entrez Gene 190534 Entrez gene
SwissProt Q95XH4 EMBL-EBI
WormBase CE22804 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB04287 Hypothetical protein F35E2.6 [Caenorhabditis elegans] ref|NP_492984.1| F35E2.6 [Caenorhabditis elegans] 0.0
blast NP_500227 Y69A2AR.10 [Caenorhabditis elegans] 0.0
blast CAB04749 Hypothetical protein T22H2.2 [Caenorhabditis elegans] ref|NP_492983.1| T22H2.2 [Caenorhabditis elegans] 0.0
blast CAB04285 Hypothetical protein F35E2.7 [Caenorhabditis elegans] ref|NP_492985.1| F35E2.7 [Caenorhabditis elegans] 1.0E-176
blast NP_500227 Y69A2AR.10 [Caenorhabditis elegans] 1.0E-169
blast NP_505161 F38E1.2 [Caenorhabditis elegans] 1.0E-152
blast AAK68582 Hypothetical protein Y69A2AR.8 [Caenorhabditis elegans] ref|NP_500229.1| Y69A2AR.8 [Caenorhabditis elegans] 2.0E-32
blast AAF59585 Hypothetical protein Y54G2A.13 [Caenorhabditis elegans] ref|NP_500256.2| Y54G2A.13 [Caenorhabditis elegans] 1.0E-24
Protein Domains
Database ID Description E-Value
Pfam IPR002656 EMBL-EBI Acyltransferase 3 family 7.7E-79
Pfam IPR000477 EMBL-EBI RNA-directed DNA polymerase (Reverse transcriptase) 3.7E-4
Pfam IPR000477 EMBL-EBI RNA-directed DNA polymerase (Reverse transcriptase) 1.5E-16
Pfam IPR002656 EMBL-EBI Acyltransferase 3 family 1.2E-28
Pfam IPR002656 EMBL-EBI Acyltransferase 3 family 1.9E-58
Pfam IPR000477 EMBL-EBI RNA-directed DNA polymerase (Reverse transcriptase) 5.9E-12
Trans Membrane
ID Number Of Domains Domain Boundaries
SNAP00000018816 5 49-71,143-162,223-240,247-269,1452-1474
GENEFINDER00000018837 9 15-32,39-58,95-117,166-185,215-237,246-263,278-293,360-382,1728-1750
Sequence
Target Sequence 
>C. ELEGANS:172074_X_AT
caatcttcattcccatggagcctgatattctgcaggcgccggtacttcccaacgttgttc
tggacaaagtgttttatgatccgacctactatgagatgtatgacattggttccgacgtgg
atgacatcgagttggatacttctttcatggtcgatccaagcacgatgacggaggaagagg
ccaccaaaaagagtaagaaaagcaagaagagctcaaagagcaagaagagcaccgacagca
ag

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CAATCTTCATTCCCATGGAGCCTGA 173 181 20 Antisense
GGAGCCTGATATTCTGCAGGCGCCG 576 521 36 Antisense
GTACTTCCCAACGTTGTTCTGGACA 238 459 61 Antisense
GTTTTATGATCCGACCTACTATGAG 348 449 90 Antisense
GATCCGACCTACTATGAGATGTATG 249 417 97 Antisense
GATGTATGACATTGGTTCCGACGTG 501 409 114 Antisense
GGTTCCGACGTGGATGACATCGAGT 173 507 127 Antisense
GACATCGAGTTGGATACTTCTTTCA 453 369 142 Antisense
GATACTTCTTTCATGGTCGATCCAA 537 423 154 Antisense
GGTCGATCCAAGCACGATGACGGAG 443 501 168 Antisense
GAGCAAGAAGAGCACCGACAGCAAG 680 389 237 Antisense
Sequence Source Affymetrix Proprietary Database