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[172065_x_at]

Description g1181594 /REP_DB=GenBank Identifier /GB=D38471.1 /PROTEIN_GB=BAA07491.1 /CHR=3 /FEA=Mapped Transcript /DEF=elongation factor Tu homologue precursor
Cluster 6grp_highest_10_10 > 63      6grp_high1_10_10 > 36      4grp_highest_8_8 > 17      4grp_high_8_8 > 61      6grp_highest_nonorm > 13      6grp_total > 35      4grp_total > 59      6grp_highest_sub > Cu      6grp_high1_sub > Cu      6grp_total_sub > Cup      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 2264
1 2 3 4 range
A 3414.3
(0.4)
P 		3492.2
(0.6)
P 		4344.4
(3)
P 		2194.7
(-3)
P 		2150
B 3208.3
(0)
P 		3727.8
(3)
P 		3523.8
(1.8)
P 		2680.7
(-3)
P 		1047
C 3153.7
(0)
P 		3602.9
(1.3)
P 		4209.8
(3)
P 		2080.2
(-3)
P 		2130
avr 3258.8
(0.3) 		3607.6
(1.5) 		4026
(3) 		2318.5
(-3) 		1708

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1714
1 2 3 4 5 6 range
A 2789.6
(2.3)
P 		2926.5
(3)
P 		2280.7
(-0.2)
P 		1893.7
(-2.1)
P 		1714.8
(-3)
P 		2279.7
(-0.2)
P 		1212
B 3106.4
(3)
P 		2872.2
(2)
P 		2973.6
(2.4)
P 		2365.3
(-0.1)
P 		1663.1
(-3)
P 		2351
(-0.1)
P 		1443
C 3273.5
(3)
P 		2995.8
(2)
P 		2650.8
(0.8)
P 		2249
(-0.6)
P 		1559.4
(-3)
P 		2438.6
(0.1)
P 		1714
avr 3056.5
(3) 		2931.5
(2.5) 		2635
(1.2) 		2169.3
(-0.8) 		1645.8
(-3) 		2356.4
(0) 		1411
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1146
1 2 3 4 range
A 1243.2
(3)
P 		758.1
(-0.2)
P 		1135.7
(2.3)
P 		321.3
(-3)
P 		922
B 1023.1
(3)
P 		920.7
(1.6)
P 		816.6
(0.3)
P 		571.3
(-3)
P 		452
C 1044
(0.7)
P 		1035.9
(0.7)
P 		1467.2
(3)
P 		350.8
(-3)
P 		1116
avr 1103.4
(2.7) 		904.9
(1.1) 		1139.8
(3) 		414.5
(-3) 		725

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 761
1 2 3 4 5 6 range
A 514.2
(-2.6)
P 		1040.8
(3)
P 		496.7
(-2.8)
P 		480.3
(-3)
P 		517.5
(-2.6)
P 		555
(-2.2)
P 		561
B 1080.9
(3)
P 		956
(1.4)
P 		722.1
(-1.7)
P 		626.5
(-3)
P 		675.7
(-2.4)
P 		876.7
(0.3)
P 		454
C 1213.3
(3)
P 		914.1
(0.6)
P 		674.7
(-1.2)
P 		452.3
(-3)
P 		504.6
(-2.6)
P 		816.3
(-0.1)
P 		761
avr 936.1
(2.5) 		970.3
(3) 		631.2
(-1.5) 		519.7
(-3) 		565.9
(-2.4) 		749.3
(0.1) 		451
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 172065_x_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.19812
Sequence Type Exemplar sequence
Representative Public ID 1181594 NCBI
Target Description g1181594 /REP_DB=GenBank Identifier /GB=D38471.1 /PROTEIN_GB=BAA07491.1 /CHR=3 /FEA=Mapped Transcript /DEF=elongation factor Tu homologue precursor
Cluster Evidence This cluster is supported by a Mapped Transcript.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_065222(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_065222 NCBI Caenorhabditis elegans Y71H2AM.23 (Y71H2AM.23) mRNA, complete cds. 11/11 A 
Y71H2AM.23.2 ENSEMBL cdna:known chromosome:CEL140:III:2811679:2814961:-1 gene:Y71H2AM.23 11/11 None
Y71H2AM.23.1 ENSEMBL cdna:known chromosome:CEL140:III:2812044:2814959:-1 gene:Y71H2AM.23 11/11 None
Y71H2AM.23.3 ENSEMBL cdna:known chromosome:CEL140:III:2812133:2814959:-1 gene:Y71H2AM.23 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000014456 6/11 Cross Hyb Matching Probes None
GENEFINDER00000014474 8/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:2812132-2814959(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl Y71H2AM.23
Entrez Gene 175398 Entrez gene
SwissProt Q19072 EMBL-EBI
WormBase CE27322 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATGENOME1:18713_AT elongation factor Tu, putative / EF-Tu, putative at
ATH1-121501:255455_AT elongation factor Tu, putative / EF-Tu, putative at
CANINE:1583725_AT similar to Tu translation elongation factor, mitochondrial cfa
CANINE_2:CFA.10170.1.A1_AT similar to Tu translation elongation factor, mitochondrial cfa
CANINE_2:CFA.10170.1.A1_S_AT similar to Tu translation elongation factor, mitochondrial cfa
CANINE_2:CFAAFFX.26342.1.S1_AT similar to Tu translation elongation factor, mitochondrial cfa
DROSOPHILA_2:1623588_AT Elongation factor Tu mitochondrial dm
DROSGENOME1:142510_AT Elongation factor Tu mitochondrial dm
HG-U133_PLUS_2:201113_AT Tu translation elongation factor, mitochondrial hs
HG-U133A_2:201113_AT Tu translation elongation factor, mitochondrial hs
HG-U133A:201113_AT Tu translation elongation factor, mitochondrial hs
HG-FOCUS:201113_AT Tu translation elongation factor, mitochondrial hs
HUGENEFL:S75463_AT Tu translation elongation factor, mitochondrial hs
U133_X3P:G4507732_3P_AT Tu translation elongation factor, mitochondrial hs
U133_X3P:HS.171379.0.A1_3P_AT Tu translation elongation factor, mitochondrial hs
HG-U133_PLUS_2:238190_AT Tu translation elongation factor, mitochondrial hs
HG-U133B:238190_AT Tu translation elongation factor, mitochondrial hs
HG-U95AV2:39867_AT Tu translation elongation factor, mitochondrial hs
HG-U95D:92041_AT Tu translation elongation factor, mitochondrial hs
MU11KSUBB:MSA.27354.0_R_AT Tu translation elongation factor, mitochondrial mm
MU11KSUBB:MSA.27354.0_F_AT Tu translation elongation factor, mitochondrial mm
MU11KSUBB:MSA.27354.0_I_AT Tu translation elongation factor, mitochondrial mm
MG-U74AV2:96626_AT Tu translation elongation factor, mitochondrial mm
MG-U74AV2:161120_R_AT Tu translation elongation factor, mitochondrial mm
MOE430A:1449672_S_AT Tu translation elongation factor, mitochondrial mm
MOUSE430A_2:1449672_S_AT Tu translation elongation factor, mitochondrial mm
MOUSE430_2:1449672_S_AT Tu translation elongation factor, mitochondrial mm
MOE430B:1447780_X_AT Tu translation elongation factor, mitochondrial mm
MOUSE430_2:1447780_X_AT Tu translation elongation factor, mitochondrial mm
MOE430A:1449673_S_AT Tu translation elongation factor, mitochondrial, mRNA (cDNA clone MGC:118710 IMAGE:6762419) mm
MOUSE430A_2:1449673_S_AT Tu translation elongation factor, mitochondrial, mRNA (cDNA clone MGC:118710 IMAGE:6762419) mm
MOUSE430_2:1449673_S_AT Tu translation elongation factor, mitochondrial, mRNA (cDNA clone MGC:118710 IMAGE:6762419) mm
MU11KSUBB:MSA.3871.0_S_AT Tu translation elongation factor, mitochondrial, mRNA (cDNA clone MGC:118710 IMAGE:6762419) mm
MU19KSUBA:TC22797_AT Tu translation elongation factor, mitochondrial, mRNA (cDNA clone MGC:118710 IMAGE:6762419) mm
MU19KSUBC:TC39794_AT Tu translation elongation factor, mitochondrial, mRNA (cDNA clone MGC:118710 IMAGE:6762419) mm
RG-U34A:RC_AA866234_AT Tu translation elongation factor, mitochondrial (predicted) rn
RAE230A:1371962_AT Tu translation elongation factor, mitochondrial (predicted) rn
RAT230_2:1371962_AT Tu translation elongation factor, mitochondrial (predicted) rn
RAE230B:1395647_AT Tu translation elongation factor, mitochondrial (predicted) rn
RAT230_2:1395647_AT Tu translation elongation factor, mitochondrial (predicted) rn
RAE230B:1395719_AT Tu translation elongation factor, mitochondrial (predicted) rn
RAT230_2:1395719_AT Tu translation elongation factor, mitochondrial (predicted) rn
RG-U34C:RC_AI175980_AT Tu translation elongation factor, mitochondrial (predicted) rn
YEAST_2:1769573_AT Mitochondrial translation elongation factor Tu; comprises both GTPase and guanine nucleotide exchange factor activities, while these activities are found in separate proteins in S. pombe and humans Sc
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6412 protein biosynthesis inferred from electronic annotation QuickGO AmiGO
6414 translational elongation inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
3746 translation elongation factor activity inferred from electronic annotation QuickGO AmiGO
5525 GTP binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAK29979 Hypothetical protein Y71H2AM.23 [Caenorhabditis elegans] ref|NP_497623.1| Y71H2AM.23 [Caenorhabditis elegans] dbj|BAA07491.1| elongation factor Tu homologue precursor [Caenorhabditis elegans] 0.0
blast BAA07492.1 elongation factor Tu homologue precursor [Caenorhabditis elegans] 0.0
blast CAE57516 Hypothetical protein CBG00491 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR004161 EMBL-EBI Elongation factor Tu, domain 2 4.7E-22
Pfam IPR000795 EMBL-EBI Protein synthesis factor, GTP-binding 4.9E-86
Pfam IPR004160 EMBL-EBI Elongation factor Tu, C-terminal 7.1E-32
Sequence
Target Sequence 
>C. ELEGANS:172065_X_AT
gacaccgattgccaactatttcagtgagcacgtcttctcattgacatgggacagtggagc
aagtgtcagaattattggaaaagactttgtgatgcctggagaatctgcggaagtcgagct
ctcactgaattcccaaatgttcatcgagccccaacaaagattcacaattcgtaaaggagc
caaaacaattggaaccggtgtgttcaccgacgttcttccaccactaaccaacgacgaaaa
ggacccgaaatcgaagaaaaagctgatgaaagctgaaatggaacgattgggattcaatcc
atacggagatatggtcgaaaaacgtttgaaaccagatttttccaattctccaaaggacaa
tccggctgcgaaggctttcgagggacaaccataaatttttcgcattttttcctgaaaatt
ttgaagaattttgtgcttaaaaatccatgtttccctcccctcctcatgatctttatagtt
attttttcctttttttagttgtagatttgagattcattgttcatatcccatttttt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GACACCGATTGCCAACTATTTCAGT 600 367 1143 Antisense
GAGCACGTCTTCTCATTGACATGGG 627 389 1168 Antisense
GAATCTGCGGAAGTCGAGCTCTCAC 398 329 1243 Antisense
GAGCTCTCACTGAATTCCCAAATGT 45 389 1258 Antisense
GTTCATCGAGCCCCAACAAAGATTC 292 445 1281 Antisense
GAACCGGTGTGTTCACCGACGTTCT 533 305 1334 Antisense
ACCAGATTTTTCCAATTCTCCAAAG 206 87 1473 Antisense
AAAGGACAATCCGGCTGCGAAGGCT 372 119 1494 Antisense
TGCTTAAAAATCCATGTTTCCCTCC 572 579 1576 Antisense
CCCCTCCTCATGATCTTTATAGTTA 240 261 1599 Antisense
ATTCATTGTTCATATCCCATTTTTT 636 11 1654 Antisense
Sequence Source GenBank