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[172059_x_at]

Description g6714902 /REP_DB=GenBank Identifier /GB=AF117300.1 /PROTEIN_GB=AAF26201.1 /CHR=X /FEA=Mapped Transcript /DEF=G protein-linked acetylcholine receptor GAR-1a
Cluster 6grp_highest_10_10 > 80      6grp_high1_10_10 > 18      4grp_highest_8_8 > 54      4grp_high_8_8 > 09      6grp_highest_nonorm > 70      6grp_total > 06      4grp_total > 22      6grp_total_sub > Decrease      6grp_cgc > 02      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 531
1 2 3 4 range
A 618
(3)
P 		253.4
(-2.2)
P 		195.5
(-3)
P 		610.7
(2.9)
P 		423
B 672.9
(3)
P 		201.1
(-2.3)
P 		142.4
(-3)
P 		488.7
(0.9)
P 		531
C 561.6
(3)
P 		233.7
(-1.8)
P 		154.1
(-3)
P 		562.8
(3)
P 		409
avr 617.5
(3) 		229.4
(-2.1) 		164
(-3) 		554.1
(2.2) 		454

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 487
1 2 3 4 5 6 range
A 676.1
(3)
P 		278
(-2.4)
P 		233.5
(-3)
P 		277.5
(-2.4)
P 		393
(-0.8)
P 		275.6
(-2.4)
P 		443
B 428.5
(2.5)
P 		445.6
(3)
P 		290
(-1.6)
P 		255.2
(-2.6)
P 		348.9
(0.1)
P 		243
(-3)
P 		203
C 317.1
(1.3)
P 		368.9
(3)
P 		189
(-3)
P 		262.5
(-0.5)
P 		215.9
(-2.1)
P 		222.6
(-1.9)
P 		180
avr 473.9
(3) 		364.2
(0.2) 		237.5
(-3) 		265.1
(-2.3) 		319.3
(-0.9) 		247.1
(-2.8) 		236
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 192
1 2 3 4 range
A 225
(3)
P 		55
(-2.9)
P 		51.1
(-3)
P 		89.4
(-1.7)
P 		174
B 214.6
(3)
P 		49.7
(-2.4)
P 		33
(-3)
P 		104.1
(-0.7)
P 		182
C 185.9
(3)
P 		67.2
(-2.4)
P 		53.7
(-3)
P 		94.9
(-1.1)
P 		132
avr 208.5
(3) 		57.3
(-2.6) 		45.9
(-3) 		96.1
(-1.1) 		163

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 101
1 2 3 4 5 6 range
A 124.6
(3)
P 		98.9
(0.9)
P 		50.9
(-3)
P 		70.4
(-1.4)
P 		118.6
(2.5)
P 		67.1
(-1.7)
P 		74
B 149.1
(3)
P 		148.3
(2.9)
P 		70.4
(-2.8)
P 		67.6
(-3)
P 		141.7
(2.5)
P 		90.6
(-1.3)
P 		82
C 117.5
(3)
P 		112.5
(2.6)
P 		48.1
(-3)
P 		52.8
(-2.6)
P 		69.9
(-1.1)
P 		74.5
(-0.7)
P 		69
avr 130.4
(3) 		119.9
(2.1) 		56.5
(-3) 		63.6
(-2.4) 		110.1
(1.4) 		77.4
(-1.3) 		74
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 172059_x_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.19999
Sequence Type Exemplar sequence
Representative Public ID 6714902 NCBI
Target Description g6714902 /REP_DB=GenBank Identifier /GB=AF117300.1 /PROTEIN_GB=AAF26201.1 /CHR=X /FEA=Mapped Transcript /DEF=G protein-linked acetylcholine receptor GAR-1a
Cluster Evidence This cluster is supported by a Mapped Transcript.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001029230(11), NM_001029231(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001029230 NCBI Caenorhabditis elegans G-protein-linked Acetylcholine Receptor family member (gar-1) (gar-1) mRNA, complete cds. 11/11 None
NM_001029231 NCBI Caenorhabditis elegans G-protein-linked Acetylcholine Receptor family member (gar-1) (gar-1) mRNA, complete cds. 11/11 None
SNAP00000029084 ENSEMBL cdna:SNAP chromosome:CEL140:X:6479593:6484765:-1 11/11 None
GENEFINDER00000029091 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:6479593:6484765:-1 10/11 None
C15B12.5b ENSEMBL cdna:known chromosome:CEL140:X:6479593:6484765:-1 gene:C15B12.5 11/11 A 
C15B12.5a ENSEMBL cdna:known chromosome:CEL140:X:6479593:6484765:-1 gene:C15B12.5 11/11 A 
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_001029232 7/11 Cross Hyb Matching Probes None
C15B12.5c 7/11 Cross Hyb Matching Probes A 
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
180922 3 2 NM_001029230 172059_x_at (11), 192401_s_at (11)
NM_001029231 172059_x_at (11), 192401_s_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:6479592-6484765(-) 100.0 100.0
Public Domain and Genome References
Ensembl C15B12.5
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAL32209 G-protein-linked acetylcholine receptor protein 1, isoform a [Caenorhabditis elegans] ref|NP_001024401.1| G-protein-linked Acetylcholine Receptor family member (gar-1) [Caenorhabditis elegans] gb|AAD13747.1| G protein-linked acetylcholine receptor GAR-1b [Caenorhabditis elegans] 0.0
blast AAL32210 G-protein-linked acetylcholine receptor protein 1, isoform b [Caenorhabditis elegans] ref|NP_001024402.1| G-protein-linked Acetylcholine Receptor family member (gar-1) [Caenorhabditis elegans] gb|AAF26201.1| G protein-linked acetylcholine receptor GAR-1a [Caenorhabditis elegans] sp|Q18007|ACM1_CAEEL Probable muscarinic acetylcholine receptor gar-1 (G-protein linked acetylcholine receptor 1) 0.0
blast T15504 hypothetical protein C15B12.5 - Caenorhabditis elegans 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR000276 EMBL-EBI Rhodopsin-like GPCR superfamily 1.5E-53
Pfam IPR000276 EMBL-EBI Rhodopsin-like GPCR superfamily 2.6E-53
Pfam IPR000276 EMBL-EBI Rhodopsin-like GPCR superfamily 3.8E-31
Pfam IPR000276 EMBL-EBI Rhodopsin-like GPCR superfamily 6.8E-17
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_001024401.1 7 20-42,63-85,100-122,143-165,192-214,603-625,640-662
NP_001024402.1 7 20-42,63-85,100-122,143-165,192-214,634-656,671-693
C15B12.5b 7 20-42,63-85,100-122,143-165,192-214,634-656,671-693
C15B12.5a 7 20-42,63-85,100-122,143-165,192-214,603-625,640-662
SNAP00000029084 7 20-42,63-85,100-122,143-165,192-214,603-625,640-662
GENEFINDER00000029091 7 20-42,63-85,100-122,143-165,192-214,618-640,655-677
Sequence
Target Sequence 
>C. ELEGANS:172059_X_AT
gcaacagcgatgattcttcgtctgaagttgagggtgaagagaaaccagaagtcagaaaca
acggcctcaaaattccccagctaaccgttaacaacgaaaatcgcggagaaacgtcttcac
agcccggacgagaccgtcttgcaccacccaataaaactgatacatttttaagcgcctcgg
gagtctccagaaaaatttcgacaatctccacggtcatcacaagggagaaggtcatttctt
caatttttgcaccgattgccgtcttcaacagaggaagaaaacagacaaaagctgaaaaga
gggctcataaagcattcagaacaatcacatttatcgtaggatttttcgcaattctttggt
caccgtattacataatggcaacagtgtatggtttctgtaaaggcgaatgcataccatcat
ttctgtacactctatcgtattacatgtgttacctgaacagtagtggtaacccatttgcct
acgctcttgccaatcgtcaattccgttcagcgttt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GCAACAGCGATGATTCTTCGTCTGA 497 319 1601 Antisense
CGCGGAGAAACGTCTTCACAGCCCG 61 255 1702 Antisense
CGTCTTGCACCACCCAATAAAACTG 640 245 1735 Antisense
ACATTTTTAAGCGCCTCGGGAGTCT 306 99 1762 Antisense
AATTTCGACAATCTCCACGGTCATC 219 175 1794 Antisense
GTCATTTCTTCAATTTTTGCACCGA 503 467 1831 Antisense
CCGATTGCCGTCTTCAACAGAGGAA 342 259 1852 Antisense
TTTTCGCAATTCTTTGGTCACCGTA 206 677 1943 Antisense
CCATCATTTCTGTACACTCTATCGT 135 271 2014 Antisense
GTAGTGGTAACCCATTTGCCTACGC 194 309 2060 Antisense
CAATCGTCAATTCCGTTCAGCGTTT 450 181 2091 Antisense
Sequence Source GenBank