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[172056_x_at]

Description Y5H2A.D /REP_DB=WormBase Gene ID /WP=CE21313 /CHR=5 /FEA=Sanger Annotation /DEF=(ST.LOUIS)
Cluster 6grp_high1_10_10 > 62      6grp_total > 10      4grp_total > 49      6grp_cgc > 65      6grp_high1_sub > 03      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 199
1 2 3 4 range
A 77.3
(-3)
A 		125.9
(-1.1)
A 		227.5
(3)
A 		133.5
(-0.8)
M 		150
B 116.9
(-0.1)
P 		194.4
(2.5)
A 		208.5
(3)
A 		28.4
(-3)
A 		180
C 102.2
(-3)
M 		195.9
(3)
A 		120.7
(-1.8)
A 		172
(1.5)
P 		94
avr 98.8
(-3) 		172.1
(2.1) 		185.6
(3) 		111.3
(-2.1) 		87

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 169
1 2 3 4 5 6 range
A 108.7
(-3)
P 		124.9
(-1.5)
M 		133.2
(-0.7)
M 		173.8
(3)
P 		126
(-1.4)
A 		128.4
(-1.2)
P 		65
B 60.9
(-3)
P 		130.9
(2.1)
P 		128.1
(1.9)
A 		130.5
(2.1)
A 		143.4
(3)
P 		61.2
(-3)
P 		83
C 90.2
(0.2)
P 		159.4
(2.8)
P 		163.8
(3)
P 		4.9
(-3)
A 		112.1
(1)
P 		157.6
(2.8)
P 		159
avr 86.6
(-3) 		138.4
(2.6) 		141.7
(3) 		103.1
(-1.2) 		127.2
(1.4) 		115.7
(0.2) 		55
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 53
1 2 3 4 range
A 28.2
(-1.7)
A 		27.3
(-1.8)
A 		59.5
(3)
A 		19.5
(-3)
M 		40
B 37.3
(1.4)
P 		48
(3)
A 		48.3
(3)
A 		6.1
(-3)
A 		42
C 33.8
(-1.9)
M 		56.3
(3)
A 		42.1
(-0.1)
A 		29
(-3)
P 		27
avr 33.1
(-0.2) 		43.9
(1.8) 		50
(3) 		18.2
(-3) 		32

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 57
1 2 3 4 5 6 range
A 20
(-3)
P 		44.4
(3)
M 		29
(-0.8)
M 		44.1
(2.9)
P 		38
(1.4)
A 		31.2
(-0.2)
P 		24
B 21.2
(-3)
P 		43.6
(0.6)
P 		31.1
(-1.4)
A 		34.6
(-0.8)
A 		58.3
(3)
P 		22.8
(-2.7)
P 		37
C 33.4
(0.8)
P 		48.6
(2.5)
P 		41.7
(1.7)
P 		1
(-3)
A 		36.3
(1.1)
P 		52.8
(3)
P 		52
avr 24.9
(-3) 		45.5
(3) 		33.9
(-0.4) 		26.6
(-2.5) 		44.2
(2.6) 		35.6
(0.1) 		21
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 172056_x_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.19522
Sequence Type Exemplar sequence
Representative Public ID Y5H2A.D NCBI
Target Description Y5H2A.D /REP_DB=WormBase Gene ID /WP=CE21313 /CHR=5 /FEA=Sanger Annotation /DEF=(ST.LOUIS)
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_182410(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_182410 NCBI Caenorhabditis elegans Activated in Blocked Unfolded protein response family member (abu-4) (abu-4) mRNA, complete cds. 11/11 A 
SNAP00000022368 ENSEMBL cdna:SNAP chromosome:CEL140:V:2274532:2275791:1 11/11 None
GENEFINDER00000022372 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:2274710:2275805:1 11/11 None
Y5H2A.3 ENSEMBL cdna:known chromosome:CEL140:V:2274710:2275805:1 gene:Y5H2A.3 11/11 A 
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_075864 1/11 Cross Hyb Matching Probes A 
GENEFINDER00000022371 4/11 Cross Hyb Matching Probes None
SNAP00000018681 1/11 Cross Hyb Matching Probes None
GENEFINDER00000018690 1/11 Cross Hyb Matching Probes None
F19G12.7 1/11 Cross Hyb Matching Probes A 
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:2274709-2275805(+) 97.16 100.0
Public Domain and Genome References
Gene Title
Gene Symbol abu-4
Ensembl Y5H2A.3
Entrez Gene 353458 Entrez gene
SwissProt Q86S74 EMBL-EBI
WormBase CE21313 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast NP_872210 Activated in Blocked Unfolded protein response family member (abu-4) [Caenorhabditis elegans] gb|AAO25980.1| Activated in blocked unfolded protein response protein 4 [Caenorhabditis elegans] 3.0E-43
blast CAB54985 Hypothetical protein Y105C5A.6 [Caenorhabditis elegans] emb|CAB54982.1| Hypothetical protein Y105C5A.3 [Caenorhabditis elegans] ref|NP_502875.1| Prion-like-(Q/N-rich)-domain-bearing protein family member (pqn-79) [Caenorhabditis elegans] ref|NP_502872.1| Prion-like-(Q/N-rich)-domain-bearing protein family member (pqn-76) [Caenorhabditis elegans] 2.0E-35
blast CAB54984 Hypothetical protein Y105C5A.5 [Caenorhabditis elegans] ref|NP_502874.1| Prion-like-(Q/N-rich)-domain-bearing protein family member (pqn-78) [Caenorhabditis elegans] 4.0E-35
Protein Domains
Database ID Description E-Value
Pfam IPR009475 EMBL-EBI Protein of unknown function DUF1096 3.1E-33
Pfam IPR009475 EMBL-EBI Protein of unknown function DUF1096 3.1E-33
Sequence
Target Sequence 
>C. ELEGANS:172056_X_AT
tgcccctcaatgccaacaggcatcggctccgcagtgccaacagtgccagaatacctgcca
gcaatttgctccagtttgccagcagcaatgtgctcctcaatgcacaatttcatcggctcc
acaatgtcagcagtgccagactacctgccagcaatttgctccagtttgtcagcaacaatg
tgctccacaatgccaacagccatcagctccacaatgccagcaatgccaatcagcatgtca
atctccagttgttgctccagttgttgctccacaaattgtgactgtcatcctagaagcttc
agtgtctcaatctgctcaatgtgaaccagaatgccaacaatcatgccaacagcaatgtgt
tcaacaacaacaaccaatgattcaatgcgctccagcttgcacccaatcatgctcacaatc
ctgctctattgctcaaccagctcagatgccatgcatgactgaatcgatcaactcctgctc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TGCCCCTCAATGCCAACAGGCATCG 488 585 498 Antisense
GTTTGCCAGCAGCAATGTGCTCCTC 432 451 571 Antisense
TCAATGCACAATTTCATCGGCTCCA 409 633 594 Antisense
CAATGTCAGCAGTGCCAGACTACCT 415 183 619 Antisense
CCAGACTACCTGCCAGCAATTTGCT 29 273 633 Antisense
GCCAATCAGCATGTCAATCTCCAGT 565 275 722 Antisense
TCCAGTTGTTGCTCCAGTTGTTGCT 306 589 741 Antisense
GAAGCTTCAGTGTCTCAATCTGCTC 354 341 790 Antisense
TTGCACCCAATCATGCTCACAATCC 334 703 894 Antisense
CTCAACCAGCTCAGATGCCATGCAT 659 227 929 Antisense
TGACTGAATCGATCAACTCCTGCTC 185 571 953 Antisense
Sequence Source Affymetrix Proprietary Database