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[172039_x_at]

Description g8489864 /REP_DB=GenBank Identifier /GB=AF271359.1 /PROTEIN_GB=AAF75788.1 /CHR=X /FEA=Mapped Transcript /DEF=STE20-like kinase MST
Cluster 6grp_highest_10_10 > 22      6grp_high1_10_10 > 02      4grp_highest_8_8 > 30      4grp_high_8_8 > 07      6grp_highest_nonorm > 61      6grp_total > 24      4grp_total > 01      6grp_highest_sub > De      6grp_high1_sub > De      6grp_total_sub > Decrease      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 617
1 2 3 4 range
A 451.9
(-3)
P 		464.9
(-2.8)
P 		466.6
(-2.8)
P 		952.2
(3)
P 		500
B 501.3
(-1)
P 		363.9
(-3)
P 		437
(-1.9)
P 		780.5
(3)
P 		417
C 445.1
(-1.7)
P 		335.3
(-3)
P 		474.6
(-1.3)
P 		825.9
(3)
P 		491
avr 466.1
(-2) 		388
(-3) 		459.4
(-2.1) 		852.9
(3) 		465

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 339
1 2 3 4 5 6 range
A 578.4
(3)
P 		522.3
(1.7)
P 		519.8
(1.7)
P 		560.3
(2.6)
P 		314.2
(-3)
P 		396.3
(-1.1)
P 		264
B 553.3
(1)
P 		499
(-0.1)
P 		653.1
(3)
P 		438
(-1.4)
P 		356.8
(-3)
P 		455.8
(-1)
P 		296
C 567.2
(1.8)
P 		627.3
(3)
P 		448.3
(-0.7)
P 		333.9
(-3)
P 		342.7
(-2.8)
P 		377.2
(-2.1)
P 		293
avr 566.3
(3) 		549.5
(2.6) 		540.4
(2.3) 		444.1
(-0.2) 		337.9
(-3) 		409.8
(-1.1) 		228
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 76
1 2 3 4 range
A 164.5
(3)
P 		100.9
(-3)
P 		122
(-1)
P 		139.4
(0.6)
P 		64
B 159.9
(2.5)
P 		89.9
(-3)
P 		101.3
(-2.1)
P 		166.3
(3)
P 		76
C 147.3
(1.4)
P 		96.4
(-3)
P 		165.4
(3)
P 		139.3
(0.7)
P 		69
avr 157.2
(3) 		95.7
(-3) 		129.6
(0.3) 		148.3
(2.1) 		62

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 143
1 2 3 4 5 6 range
A 106.6
(-2.2)
P 		185.8
(3)
P 		113.2
(-1.8)
P 		142.1
(0.1)
P 		94.8
(-3)
P 		96.5
(-2.9)
P 		91
B 192.5
(3)
P 		166.1
(0.9)
P 		158.6
(0.3)
P 		116
(-3)
P 		145
(-0.7)
P 		170
(1.2)
P 		77
C 210.2
(3)
P 		191.4
(2.2)
P 		114.1
(-1)
P 		67.1
(-3)
P 		110.9
(-1.2)
P 		126.3
(-0.5)
P 		143
avr 169.8
(2.1) 		181.1
(3) 		128.6
(-1.3) 		108.4
(-3) 		116.9
(-2.3) 		130.9
(-1.1) 		73
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 172039_x_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.19982
Sequence Type Exemplar sequence
Representative Public ID 8489864 NCBI
Target Description g8489864 /REP_DB=GenBank Identifier /GB=AF271359.1 /PROTEIN_GB=AAF75788.1 /CHR=X /FEA=Mapped Transcript /DEF=STE20-like kinase MST
Cluster Evidence This cluster is supported by a Mapped Transcript.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_076342(9), NM_001029404(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_076342 NCBI Caenorhabditis elegans F14H12.4a (F14H12.4) mRNA, complete cds. 9/11 A 
NM_001029404 NCBI Caenorhabditis elegans F14H12.4b (F14H12.4) mRNA, complete cds. 11/11 None
GENEFINDER00000002247 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:4339618:4343557:1 9/11 None
F14H12.4b ENSEMBL cdna:known chromosome:CEL140:X:4339534:4343494:1 gene:F14H12.4 11/11 A 
F14H12.4a ENSEMBL cdna:known chromosome:CEL140:X:4339534:4343610:1 gene:F14H12.4 9/11 A 
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_076340 8/11 Cross Hyb Matching Probes A 
NM_076341 6/11 Cross Hyb Matching Probes A 
SNAP00000002228 3/11 Cross Hyb Matching Probes None
GENEFINDER00000002241 3/11 Cross Hyb Matching Probes None
SNAP00000002231 8/11 Cross Hyb Matching Probes None
C24A8.4a 8/11 Cross Hyb Matching Probes A 
C24A8.4b 6/11 Cross Hyb Matching Probes A 
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:4339618-4343495(+) 100.0 100.0
chrX:4333206-4336009(-) 82.02 82.02
Public Domain and Genome References
Ensembl F14H12.4
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAK68326 Hypothetical protein F14H12.4a [Caenorhabditis elegans] ref|NP_508743.3| F14H12.4a [Caenorhabditis elegans] 0.0
blast AAK68327 Hypothetical protein F14H12.4b [Caenorhabditis elegans] ref|NP_001024575.1| F14H12.4b [Caenorhabditis elegans] 0.0
blast AAF75788 STE20-like kinase MST [Caenorhabditis elegans] 0.0
Protein Families
Method ID Description E-Value
ec A2S7_HUMAN (Q96Q40) Serine/threonine-protein kinase ALS2CR7 (EC 2.7.1.37) (Amyotrophic lateral sclerosis 2 chromosomal region candidate gene protein 7) 1.0E-64
ec A2S7_HUMAN (Q96Q40) Serine/threonine-protein kinase ALS2CR7 (EC 2.7.1.37) (Amyotrophic lateral sclerosis 2 chromosomal region candidate gene protein 7) 3.0E-65
hanks 2.1.17 CaMK Group; CaMK I Regulated by Ca2+/CaM and close relatives; TWITCH 1.0E-71
hanks 2.1.17 CaMK Group; CaMK I Regulated by Ca2+/CaM and close relatives; TWITCH 6.0E-72
Protein Domains
Database ID Description E-Value
Pfam IPR000719 EMBL-EBI Protein kinase 8.5E-93
Pfam IPR000719 EMBL-EBI Protein kinase 8.5E-93
Sequence
Target Sequence 
>C. ELEGANS:172039_X_AT
agcaccaatggcagcaagcagcggaaatgatgcaacgttgctaagcgagggaatgtccac
tatgattgacggtggagagtctacgttagttcaacacaaagacaactatgtcactgctca
aagtcttcgaagtcaaatggaaagcttgagaattggtggagaaattcctaaatcggcata
tattccaggctcgtcgaaaaatggcaactcaccgcgcgttcaaccaccgggtcacaccgc
aagcgcttcagacccttcaaaaaaccaaccgtttgctcaagatggaaccggtccaaactt
tcaattgggcaccagcgagagcagttacaaagatgctagttataatatgatgaacacgga
agccgaatacgaaaatagatttcaacgggcagtggtcgatgg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AGCACCAATGGCAGCAAGCAGCGGA 14 77 942 Antisense
GGGAATGTCCACTATGATTGACGGT 70 495 990 Antisense
GGAGAGTCTACGTTAGTTCAACACA 286 517 1015 Antisense
GTCACTGCTCAAAGTCTTCGAAGTC 217 465 1051 Antisense
AAATCGGCATATATTCCAGGCTCGT 131 119 1111 Antisense
GCATATATTCCAGGCTCGTCGAAAA 287 309 1117 Antisense
GTCGAAAAATGGCAACTCACCGCGC 265 475 1134 Antisense
AACCGTTTGCTCAAGATGGAACCGG 491 145 1208 Antisense
TGGAACCGGTCCAAACTTTCAATTG 104 545 1224 Antisense
ATTGGGCACCAGCGAGAGCAGTTAC 226 3 1245 Antisense
GATTTCAACGGGCAGTGGTCGATGG 325 427 1319 Antisense
Sequence Source GenBank