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[172038_x_at]

Description g385198 /REP_DB=GenBank Identifier /GB=L19621.1 /PROTEIN_GB=AAC14456.1 /CHR=4 /FEA=Mapped Transcript /DEF=acetylcholine transporter
Cluster 6grp_highest_10_10 > 70      6grp_high1_10_10 > 17      4grp_highest_8_8 > 58      4grp_high_8_8 > 33      6grp_highest_nonorm > 50      6grp_total > 07      4grp_total > 46      6grp_highest_sub > De      6grp_cgc > 12      6grp_high1_sub > De      6grp_cgc_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S1      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 856
1 2 3 4 range
A 803.2
(3)
P 		550
(-0.2)
P 		331.1
(-3)
P 		413.4
(-2)
P 		472
B 1187.2
(3)
P 		538.7
(-1.6)
P 		348.1
(-3)
P 		381
(-2.8)
P 		839
C 899.6
(3)
P 		574.4
(-0.6)
P 		403.1
(-2.5)
P 		355.2
(-3)
P 		544
avr 963.3
(3) 		554.4
(-1.1) 		360.8
(-3) 		383.2
(-2.8) 		603

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 910
1 2 3 4 5 6 range
A 1083.3
(3)
P 		453.1
(-1.2)
P 		261.8
(-2.4)
P 		173.7
(-3)
P 		273.4
(-2.3)
P 		225
(-2.7)
P 		910
B 951.8
(3)
P 		542.7
(-0.2)
P 		261.9
(-2.4)
P 		240.3
(-2.6)
P 		184.1
(-3)
P 		189.3
(-3)
P 		768
C 978.1
(3)
P 		426.8
(-1.1)
P 		279.5
(-2.2)
P 		220.8
(-2.7)
P 		211.9
(-2.7)
P 		177
(-3)
P 		801
avr 1004.4
(3) 		474.2
(-0.9) 		267.7
(-2.5) 		211.6
(-2.9) 		223.1
(-2.8) 		197.1
(-3) 		807
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 319
1 2 3 4 range
A 292.4
(3)
P 		119.4
(-1.5)
P 		86.6
(-2.3)
P 		60.5
(-3)
P 		232
B 378.6
(3)
P 		133.1
(-1.9)
P 		80.7
(-3)
P 		81.2
(-3)
P 		298
C 297.8
(3)
P 		165.1
(-0.3)
P 		140.5
(-1)
P 		59.9
(-3)
P 		238
avr 322.9
(3) 		139.2
(-1.3) 		102.6
(-2.2) 		67.2
(-3) 		256

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 318
1 2 3 4 5 6 range
A 199.7
(3)
P 		161.2
(1.5)
P 		57
(-2.5)
P 		44.1
(-3)
P 		82.5
(-1.5)
P 		54.8
(-2.6)
P 		156
B 331.2
(3)
P 		180.6
(-0.4)
P 		63.6
(-3)
P 		63.7
(-3)
P 		74.8
(-2.7)
P 		70.6
(-2.8)
P 		268
C 362.5
(3)
P 		130.2
(-1.4)
P 		71.1
(-2.5)
P 		44.4
(-3)
P 		68.6
(-2.5)
P 		59.2
(-2.7)
P 		318
avr 297.8
(3) 		157.3
(-0.4) 		63.9
(-2.7) 		50.7
(-3) 		75.3
(-2.4) 		61.5
(-2.7) 		247
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 172038_x_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.19879
Sequence Type Exemplar sequence
Representative Public ID 385198 NCBI
Target Description g385198 /REP_DB=GenBank Identifier /GB=L19621.1 /PROTEIN_GB=AAC14456.1 /CHR=4 /FEA=Mapped Transcript /DEF=acetylcholine transporter
Cluster Evidence This cluster is supported by a Mapped Transcript.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001028431(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001028431 NCBI Caenorhabditis elegans UNCoordinated family member (unc-17) (unc-17) mRNA, complete cds. 11/11 None
GENEFINDER00000026358 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:3618495:3627846:-1 9/11 None
ZC416.8a ENSEMBL cdna:known chromosome:CEL140:IV:3618250:3624692:-1 gene:ZC416.8 11/11 A 
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000026351 8/11 Cross Hyb Matching Probes None
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
177124 2 1 NM_001028431 172038_x_at (11), 192428_s_at (11), 194229_x_at (11)
NM_001028432 172037_x_at (11), 188870_s_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:3618255-3624704(-) 96.32 100.0
Public Domain and Genome References
Ensembl ZC416.8
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast NP_001023602 UNCoordinated family member (unc-17) [Caenorhabditis elegans] gb|AAB88371.1| Uncoordinated protein 17, isoform a [Caenorhabditis elegans] sp|P34711|UNC17_CAEEL Vesicular acetylcholine transporter unc-17 (Uncoordinated protein 17) gb|AAC14456.1| acetylcholine transporter [Caenorhabditis elegans] gb|AAC13764.1| UNC-17 0.0
blast CAE63770 Hypothetical protein CBG08307 [Caenorhabditis briggsae] 0.0
blast EAL27592 GA17179-PA [Drosophila pseudoobscura] 1.0E-144
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_001023602.1 10 33-55,128-150,189-211,221-243,264-286,301-323,336-358,362-384,405-422,426-448
ZC416.8a 10 33-55,128-150,189-211,221-243,264-286,301-323,336-358,362-384,405-422,426-448
GENEFINDER00000026358 10 115-137,210-232,271-293,303-325,346-368,383-405,418-440,444-466,487-504,508-530
Sequence
Target Sequence 
>C. ELEGANS:172038_X_AT
tactcgttggcttacgcctttggacccatcattgccggatggattgtgaccaactgggga
ttcaccgccctcaacatcataatcttcgccaccaacgtcacctacgccccagttttattc
ctactgcggaaagttcactcctacgacaccctcggcgccaagggtgacaccgccgaaatg
acacagctcaactcatcggctccagctggcggatacaatgggaagcctgaagcaactaca
gcggaatcctatcaaggatgggaagatcagcagtcgtaccagaatcaggcacagatcccg
aaccacgccgtgtcgttccaggattcaaggccccaagccgaattccccgccggatatgac
ccactgaatccgcagtggtagtcgtagatttggatctctgaatatgtttattctaccaag
tgcaatggccaaaatgactagcctagattgctttatttccttttttctttcagatttctc
ttctttttttgtgtcctttcatccttctctgttactctacaaat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TACTCGTTGGCTTACGCCTTTGGAC 128 647 1317 Antisense
TGCGGAAAGTTCACTCCTACGACAC 705 581 1441 Antisense
TGACACCGCCGAAATGACACAGCTC 691 571 1481 Antisense
GGCTCCAGCTGGCGGATACAATGGG 84 541 1514 Antisense
GAAGCAACTACAGCGGAATCCTATC 211 339 1545 Antisense
GGGAAGATCAGCAGTCGTACCAGAA 75 493 1576 Antisense
CAGAATCAGGCACAGATCCCGAACC 438 203 1596 Antisense
CGCCGTGTCGTTCCAGGATTCAAGG 185 257 1622 Antisense
GGATATGACCCACTGAATCCGCAGT 688 509 1668 Antisense
TGCTTTATTTCCTTTTTTCTTTCAG 40 579 1765 Antisense
CATCCTTCTCTGTTACTCTACAAAT 329 211 1816 Antisense
Sequence Source GenBank