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[172037_x_at]

Description g156241 /REP_DB=GenBank Identifier /GB=L08969.1 /PROTEIN_GB=AAA53659.1 /CHR=4 /FEA=Mapped Transcript /DEF=choline acetyltransferase
Cluster 6grp_highest_10_10 > 40      6grp_high1_10_10 > 05      4grp_high_8_8 > 18      6grp_highest_nonorm > 80      6grp_total > 06      4grp_total > 30      6grp_highest_sub > De      6grp_total_sub > Decrease      6grp_cgc > 22      6grp_high1_sub > De      6grp_cgc_sub > De      6grp_rule_based > S1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 157
1 2 3 4 range
A 255.2
(3)
P 		180
(-1.4)
P 		152.8
(-3)
A 		255.6
(3)
P 		103
B 291.6
(3)
P 		177.8
(-2.2)
P 		186.8
(-1.8)
A 		160.9
(-3)
P 		131
C 265.8
(3)
P 		188.6
(-0.5)
P 		134.8
(-3)
P 		249.1
(2.2)
P 		131
avr 270.9
(3) 		182.1
(-1.7) 		158.1
(-3) 		221.9
(0.4) 		113

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 190
1 2 3 4 5 6 range
A 307.5
(3)
P 		198.4
(-0.9)
P 		167.2
(-2.1)
P 		141.7
(-3)
P 		184.2
(-1.5)
P 		200.3
(-0.9)
P 		166
B 301.2
(3)
P 		272.2
(1.9)
P 		221.3
(-0)
P 		163.3
(-2.2)
P 		166.6
(-2.1)
P 		142.8
(-3)
P 		158
C 302.9
(3)
P 		273
(2)
P 		202.8
(-0.2)
P 		175.9
(-1.1)
P 		186.7
(-0.8)
P 		118
(-3)
P 		185
avr 303.9
(3) 		247.9
(0.8) 		197.1
(-1.3) 		160.3
(-2.7) 		179.2
(-2) 		153.7
(-3) 		150
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 59
1 2 3 4 range
A 92.9
(3)
P 		39.1
(-2.8)
P 		39.9
(-2.7)
A 		37.4
(-3)
P 		56
B 93
(3)
P 		43.9
(-2)
P 		43.3
(-2.1)
A 		34.3
(-3)
P 		59
C 88
(3)
P 		54.2
(-1.4)
P 		47
(-2.3)
P 		42
(-3)
P 		46
avr 91.3
(3) 		45.7
(-2.1) 		43.4
(-2.4) 		37.9
(-3) 		53

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 77
1 2 3 4 5 6 range
A 56.7
(0.6)
P 		70.6
(3)
P 		36.4
(-2.9)
P 		35.9
(-3)
P 		55.6
(0.4)
P 		48.8
(-0.8)
P 		35
B 104.8
(3)
P 		90.6
(1.6)
P 		53.7
(-2)
P 		43.3
(-3)
P 		67.7
(-0.6)
P 		53.2
(-2)
P 		62
C 112.3
(3)
P 		83.3
(0.7)
P 		51.6
(-1.7)
P 		35.4
(-3)
P 		60.4
(-1)
P 		39.5
(-2.7)
P 		77
avr 91.3
(3) 		81.5
(1.9) 		47.2
(-2) 		38.2
(-3) 		61.2
(-0.4) 		47.2
(-2) 		53
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 172037_x_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.19887
Sequence Type Exemplar sequence
Representative Public ID 156241 NCBI
Target Description g156241 /REP_DB=GenBank Identifier /GB=L08969.1 /PROTEIN_GB=AAA53659.1 /CHR=4 /FEA=Mapped Transcript /DEF=choline acetyltransferase
Cluster Evidence This cluster is supported by a Mapped Transcript.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001028432(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001028432 NCBI Caenorhabditis elegans UNCoordinated family member (unc-17) (unc-17) mRNA, complete cds. 11/11 None
SNAP00000026351 ENSEMBL cdna:SNAP chromosome:CEL140:IV:3614049:3632550:-1 11/11 None
GENEFINDER00000026360 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:3613818:3617770:-1 11/11 None
ZC416.8b ENSEMBL cdna:known chromosome:CEL140:IV:3613927:3624692:-1 gene:ZC416.8 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
177124 2 1 NM_001028431 172038_x_at (11), 192428_s_at (11), 194229_x_at (11)
NM_001028432 172037_x_at (11), 188870_s_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:3613932-3624700(-) 98.02 100.0
Public Domain and Genome References
Ensembl ZC416.8
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast NP_001023603 UNCoordinated family member (unc-17) [Caenorhabditis elegans] gb|AAB88370.1| Abnormal choline acetyltransferase protein 1, isoform b [Caenorhabditis elegans] sp|P32756|CLAT_CAEEL Choline O-acetyltransferase (CHOACTase) (Choline acetylase) (ChAT) gb|AAA53660.1| choline acetyltransferase gb|AAA53659.1| choline acetyltransferase 0.0
blast CAE63768 Hypothetical protein CBG08305 [Caenorhabditis briggsae] 0.0
blast NP_001023602 UNCoordinated family member (unc-17) [Caenorhabditis elegans] gb|AAB88371.1| Uncoordinated protein 17, isoform a [Caenorhabditis elegans] sp|P34711|UNC17_CAEEL Vesicular acetylcholine transporter unc-17 (Uncoordinated protein 17) gb|AAC14456.1| acetylcholine transporter [Caenorhabditis elegans] gb|AAC13764.1| UNC-17 0.0
blast CAD44849 choline-o-acetyltransferase [Echinococcus multilocularis] 2.0E-96
Protein Domains
Database ID Description E-Value
Pfam IPR000542 EMBL-EBI Acyltransferase ChoActase/COT/CPT 1.0E-126
Pfam IPR000542 EMBL-EBI Acyltransferase ChoActase/COT/CPT 1.0E-126
Pfam IPR000542 EMBL-EBI Acyltransferase ChoActase/COT/CPT 1.0E-104
Pfam IPR000542 EMBL-EBI Acyltransferase ChoActase/COT/CPT 1.0E-126
Trans Membrane
ID Number Of Domains Domain Boundaries
SNAP00000026351 11 39-61,134-156,163-185,200-222,229-247,267-289,309-331,341-361,368-390,405-427,432-454
GENEFINDER00000026360 1 612-634
Sequence
Target Sequence 
>C. ELEGANS:172037_X_AT
gaacatcagcggctacggtgtcgacaatcatctttgcgccctattctgtctcgcccggga
aagggaagaaacgaccggagaagatataccatcactgtttctggatcctctgtggtctga
agttatgcgattcccattgtcgacaagccaagtcacaacctctctggatatccctgattg
ctacctgacgtatggtgcagtggtccgcgatggctatggatgcccgtacaatattcaacc
ggatcgagtgatttttgcgccgactgcgttcagaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAACATCAGCGGCTACGGTGTCGAC 410 351 1558 Antisense
ACGGTGTCGACAATCATCTTTGCGC 58 97 1572 Antisense
TATTCTGTCTCGCCCGGGAAAGGGA 161 659 1599 Antisense
TATACCATCACTGTTTCTGGATCCT 419 661 1642 Antisense
CTGGATCCTCTGTGGTCTGAAGTTA 528 239 1658 Antisense
GTTATGCGATTCCCATTGTCGACAA 406 447 1679 Antisense
TCGACAAGCCAAGTCACAACCTCTC 158 601 1697 Antisense
CAACCTCTCTGGATATCCCTGATTG 487 187 1713 Antisense
TGACGTATGGTGCAGTGGTCCGCGA 271 571 1743 Antisense
TGGTCCGCGATGGCTATGGATGCCC 223 553 1758 Antisense
ATTTTTGCGCCGACTGCGTTCAGAA 430 15 1808 Antisense
Sequence Source GenBank