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[172036_x_at] alanyl-tRNA synthetase

Description g6176435 /REP_DB=GenBank Identifier /GB=AF188715.1 /PROTEIN_GB=AAF05590.1 /CHR=2 /FEA=Mapped Transcript /DEF=alanyl-tRNA synthetase
Cluster 6grp_highest_10_10 > 44      6grp_high1_10_10 > 43      4grp_highest_8_8 > 48      4grp_high_8_8 > 42      6grp_highest_nonorm > 90      6grp_total > 40      4grp_total > 45      6grp_highest_sub > 02      6grp_cgc > 72      6grp_high1_sub > 02      6grp_cgc_sub > 02      6grp_total_sub > Hat      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 130
1 2 3 4 range
A 185.5
(3)
P 		115.2
(-1.9)
P 		99.6
(-3)
P 		100.9
(-2.9)
P 		86
B 176.4
(3)
P 		165.7
(2.4)
P 		101.2
(-1.2)
P 		69.2
(-3)
P 		107
C 198.8
(3)
P 		163.6
(0.4)
P 		143.7
(-1.1)
P 		117.7
(-3)
P 		81
avr 186.9
(3) 		148.2
(0.4) 		114.8
(-1.8) 		95.9
(-3) 		91

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 121
1 2 3 4 5 6 range
A 103.2
(-3)
P 		180.3
(2.3)
P 		161.1
(0.9)
P 		191.2
(3)
P 		150.3
(0.2)
P 		140.7
(-0.4)
P 		88
B 200.6
(1.8)
P 		179.4
(0.2)
P 		217.9
(3)
P 		153.3
(-1.7)
P 		146.6
(-2.1)
P 		134.6
(-3)
P 		83
C 157.6
(1)
P 		183.9
(2.7)
P 		147.8
(0.3)
P 		189
(3)
P 		96.8
(-3)
P 		162.1
(1.2)
P 		92
avr 153.8
(-0.3) 		181.2
(3) 		175.6
(2.3) 		177.8
(2.6) 		131.2
(-3) 		145.8
(-1.2) 		50
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 53
1 2 3 4 range
A 67.5
(3)
P 		25
(-1.8)
P 		26
(-1.7)
P 		14.8
(-3)
P 		53
B 56.3
(3)
P 		40.9
(0.8)
P 		23.5
(-1.7)
P 		14.8
(-3)
P 		42
C 65.8
(3)
P 		47
(0.5)
P 		50.1
(1)
P 		19.8
(-3)
P 		46
avr 63.2
(3) 		37.6
(-0.3) 		33.2
(-0.9) 		16.5
(-3) 		47

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 51
1 2 3 4 5 6 range
A 19
(-3)
P 		64.1
(3)
P 		35.1
(-0.9)
P 		48.5
(0.9)
P 		45.4
(0.5)
P 		34.3
(-1)
P 		45
B 69.8
(3)
P 		59.7
(0.9)
P 		52.9
(-0.5)
P 		40.6
(-3)
P 		59.5
(0.9)
P 		50.2
(-1)
P 		29
C 58.4
(3)
P 		56.1
(2.5)
P 		37.6
(-1.6)
P 		38
(-1.5)
P 		31.3
(-3)
P 		54.3
(2.1)
P 		27
avr 49.1
(-0.6) 		60
(3) 		41.9
(-3) 		42.4
(-2.8) 		45.4
(-1.8) 		46.3
(-1.5) 		18
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 172036_x_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.19723
Sequence Type Exemplar sequence
Representative Public ID 6176435 NCBI
Target Description g6176435 /REP_DB=GenBank Identifier /GB=AF188715.1 /PROTEIN_GB=AAF05590.1 /CHR=2 /FEA=Mapped Transcript /DEF=alanyl-tRNA synthetase
Cluster Evidence This cluster is supported by a Mapped Transcript.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_064043(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_064043 NCBI Caenorhabditis elegans Alanyl tRNA Synthetase family member (ars-1) (ars-1) mRNA, complete cds. 11/11 A 
SNAP00000002910 ENSEMBL cdna:SNAP chromosome:CEL140:II:11460235:11463926:-1 11/11 None
GENEFINDER00000002921 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:11460235:11463926:-1 11/11 None
W02B12.6 ENSEMBL cdna:known chromosome:CEL140:II:11460137:11463981:-1 gene:W02B12.6 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:11460133-11463978(-) 100.0 100.0
Public Domain and Genome References
Gene Title alanyl-tRNA synthetase
Gene Symbol ars-1
Ensembl W02B12.6
Entrez Gene 174749 Entrez gene
SwissProt Q23122 EMBL-EBI
WormBase CE28096 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6419 alanyl-tRNA aminoacylation inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4813 alanine-tRNA ligase activity inferred from electronic annotation QuickGO AmiGO
5524 ATP binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA91396 Hypothetical protein W02B12.6 [Caenorhabditis elegans] ref|NP_496444.1| Alanyl tRNA Synthetase family member (ars-1) [Caenorhabditis elegans] gb|AAF05590.1| alanyl-tRNA synthetase [Caenorhabditis elegans] 0.0
blast CAE59502 Hypothetical protein CBG02888 [Caenorhabditis briggsae] 0.0
blast CAE66735 Hypothetical protein CBG12085 [Caenorhabditis briggsae] 1.0E-152
Protein Domains
Database ID Description E-Value
Pfam IPR002318 EMBL-EBI Alanyl-tRNA synthetase, class IIc 1.0E-126
Sequence
Target Sequence 
>C. ELEGANS:172036_X_AT
gactgcgatcgcttcagatttgactattccacgggagacgaagatttgagcaaggagcaa
agaacggaacttttgattaaatgtgaaatgaaaatgagggaattcattcaaaatggaggt
ttcacagaaattatagagacgagtttggaagaagcaaagaaaatcgaaaatctgcaatct
gatgtgaaagaggatagaattggaggcgcttcagtgagagttgtggctttaggcagtgga
gctgatgtgccagtggagtgctgttctggaacacacatccatgacgtcagagtgattgat
gacgtggcaattatgtcggacaagtcaatgggtcaaaggcttcgaagaattatagtcctc
actggaaaggaagcagctgcgtgcagaaactatacaaaaagcatttatgaagatttaagg
agtacggatccaaaggagagatcaaaaacgggaaagaacatcgactggaaacgtgtccca
attgctgatcaagctcgtatctcgattttgctga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GACTGCGATCGCTTCAGATTTGACT 288 375 1922 Antisense
CAGATTTGACTATTCCACGGGAGAC 632 203 1936 Antisense
AATTGGAGGCGCTTCAGTGAGAGTT 113 179 2119 Antisense
GAGAGTTGTGGCTTTAGGCAGTGGA 430 395 2137 Antisense
GCAGTGGAGCTGATGTGCCAGTGGA 569 311 2154 Antisense
GATGTGCCAGTGGAGTGCTGTTCTG 403 409 2165 Antisense
GAACACACATCCATGACGTCAGAGT 206 353 2190 Antisense
GGCTTCGAAGAATTATAGTCCTCAC 525 537 2259 Antisense
GAACATCGACTGGAAACGTGTCCCA 235 349 2377 Antisense
ACGTGTCCCAATTGCTGATCAAGCT 563 95 2392 Antisense
CAAGCTCGTATCTCGATTTTGCTGA 689 185 2411 Antisense
Sequence Source GenBank