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[171999_x_at]

Description g6451494 /REP_DB=GenBank Identifier /GB=D84668.1 /PROTEIN_GB=BAA86913.1 /CHR=2 /FEA=Mapped Transcript /DEF=CLH-1
Cluster 6grp_highest_10_10 > 92      6grp_high1_10_10 > 49      4grp_highest_8_8 > 23      4grp_high_8_8 > 13      6grp_highest_nonorm > 77      6grp_total > 49      4grp_total > 11      6grp_highest_sub > Cu      6grp_cgc > 08      6grp_high1_sub > Cu      6grp_cgc_sub > Cu      6grp_total_sub > Cup      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1508
1 2 3 4 range
A 1049.2
(-2.5)
P 		1242.6
(-1.6)
P 		935.9
(-3)
P 		2245.4
(3)
P 		1310
B 1138.2
(-2.1)
P 		1053.6
(-2.5)
P 		922.2
(-3)
P 		2430.3
(3)
P 		1508
C 1094.3
(-2.6)
P 		1028.1
(-3)
P 		1062.7
(-2.8)
P 		2050.6
(3)
P 		1023
avr 1093.9
(-2.4) 		1108.1
(-2.4) 		973.6
(-3) 		2242.1
(3) 		1269

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 926
1 2 3 4 5 6 range
A 1840.9
(3)
P 		1066.5
(-2)
P 		915.1
(-3)
P 		957.4
(-2.7)
P 		1646.1
(1.7)
P 		1526.1
(1)
P 		926
B 1274.9
(-0.8)
P 		1162.7
(-3)
P 		1308
(-0.1)
P 		1405.7
(1.8)
P 		1309.5
(-0.1)
P 		1467.7
(3)
P 		305
C 1205.7
(-1.1)
P 		1244.6
(-0.2)
P 		1272.1
(0.4)
P 		1124.9
(-3)
P 		1385.2
(3)
P 		1352.4
(2.2)
P 		260
avr 1440.5
(2.8) 		1157.9
(-3) 		1165.1
(-2.9) 		1162.7
(-2.9) 		1446.9
(3) 		1448.7
(3) 		291
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 304
1 2 3 4 range
A 382
(3)
P 		269.7
(-1.9)
P 		244.7
(-3)
P 		328.7
(0.7)
P 		137
B 363
(-0.1)
P 		260.2
(-2.1)
P 		213.7
(-3)
P 		517.9
(3)
P 		304
C 362.2
(2.3)
P 		295.6
(-3)
P 		370.4
(3)
P 		345.8
(1)
P 		75
avr 369.1
(1.6) 		275.2
(-3) 		276.3
(-2.9) 		397.5
(3) 		122

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 348
1 2 3 4 5 6 range
A 339.4
(-0.2)
P 		379.3
(0.6)
P 		199.3
(-3)
P 		242.8
(-2.1)
P 		496.7
(3)
P 		371.6
(0.5)
P 		297
B 443.6
(0.3)
P 		387
(-1.2)
P 		317.6
(-3)
P 		372.3
(-1.6)
P 		532
(2.6)
P 		547.3
(3)
P 		230
C 446.9
(2.8)
P 		379.8
(1.1)
P 		323.8
(-0.4)
P 		226.2
(-3)
P 		448.2
(2.9)
P 		452.7
(3)
P 		227
avr 410
(0.7) 		382
(-0.1) 		280.2
(-3) 		280.4
(-3) 		492.3
(3) 		457.2
(2) 		212
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 171999_x_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.19707
Sequence Type Exemplar sequence
Representative Public ID 6451494 NCBI
Target Description g6451494 /REP_DB=GenBank Identifier /GB=D84668.1 /PROTEIN_GB=BAA86913.1 /CHR=2 /FEA=Mapped Transcript /DEF=CLH-1
Cluster Evidence This cluster is supported by a Mapped Transcript.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001027203(11), NM_001027202(10)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001027203 NCBI Caenorhabditis elegans CLC-type chloride cHannel family member (clh-1) (clh-1) mRNA, complete cds. 11/11 None
NM_001027202 NCBI Caenorhabditis elegans CLC-type chloride cHannel family member (clh-1) (clh-1) mRNA, complete cds. 10/11 None
T27D12.2b ENSEMBL cdna:known chromosome:CEL140:II:11846413:11854648:-1 gene:T27D12.2 11/11 A 
T27D12.2a ENSEMBL cdna:known chromosome:CEL140:II:11846592:11854648:-1 gene:T27D12.2 10/11 A 
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000038205 7/11 Cross Hyb Matching Probes None
GENEFINDER00000038211 7/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:11846409-11854645(-) 99.05 100.0
Public Domain and Genome References
Ensembl T27D12.2
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAC42352 Hypothetical protein T27D12.2b [Caenorhabditis elegans] ref|NP_001022374.1| CLC-type chloride cHannel family member (clh-1) [Caenorhabditis elegans] dbj|BAA86913.1| CLH-1 [Caenorhabditis elegans] 0.0
blast CAA93879 Hypothetical protein T27D12.2a [Caenorhabditis elegans] ref|NP_001022373.1| CLC-type chloride cHannel family member (clh-1) [Caenorhabditis elegans] 0.0
blast AAF13163 CLC chloride channel protein [Caenorhabditis elegans] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR000644 EMBL-EBI CBS domain 1.7E-4
Pfam IPR000644 EMBL-EBI CBS domain 0.0021
Pfam IPR001807 EMBL-EBI Cl- channel, voltage gated 1.0E-126
Pfam IPR000644 EMBL-EBI CBS domain 0.0021
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_001022374.1 10 120-142,163-185,276-298,324-346,361-380,401-423,473-495,502-524,539-561,568-590
NP_001022373.1 10 120-142,163-185,276-298,324-346,361-380,401-423,473-495,502-524,539-561,568-590
T27D12.2b 10 120-142,163-185,276-298,324-346,361-380,401-423,473-495,502-524,539-561,568-590
T27D12.2a 10 120-142,163-185,276-298,324-346,361-380,401-423,473-495,502-524,539-561,568-590
Sequence
Target Sequence 
>C. ELEGANS:171999_X_AT
tgctctcaaccatgcctatgttactgaaaaaggtcgtctttgtggtgtagttgccgtgaa
ggaacttcgtgaggcgctcagtaacatctacagtagaggagctgtcgtcccgaaacagag
aatccgaacatccacattccgactcaatccagaacaagatcttgaccgtgagctccaaaa
cggccatacagcggagccaaacggcgccccaatcagacaaccacgtgtcacaatcgatgt
tcctgaaaacgcaaagacggatgacgaaacccgctaaattttgtgaaaaatttcaaattc
tagtcaaccaaacagtcaatcattccttccattattattattattttaaagcgtcccccc
cggttgtttcctctttctctcccagtttctgtaagtagttcttctctccaaaaaaacaaa
acaacgagttgttcccaattccccagacgttcctaaggaattgtgggaaaaattaaaatt
aaaattaaaattaaaaccgaatacataattcattcattgaaaaatcccgatttgtatata
gcagtcataatttcataatttctcctgatgagtttcccgtga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TGCTCTCAACCATGCCTATGTTACT 186 581 2446 Antisense
GGTCGTCTTTGTGGTGTAGTTGCCG 266 503 2477 Antisense
GTGAAGGAACTTCGTGAGGCGCTCA 449 479 2501 Antisense
GAGGCGCTCAGTAACATCTACAGTA 343 405 2516 Antisense
AGAGAATCCGAACATCCACATTCCG 519 69 2562 Antisense
AGATCTTGACCGTGAGCTCCAAAAC 44 67 2602 Antisense
ACCACGTGTCACAATCGATGTTCCT 109 85 2665 Antisense
AAACAGTCAATCATTCCTTCCATTA 236 129 2755 Antisense
TCTTTCTCTCCCAGTTTCTGTAAGT 257 615 2817 Antisense
TTCTGTAAGTAGTTCTTCTCTCCAA 659 693 2832 Antisense
ATTTCTCCTGATGAGTTTCCCGTGA 81 17 3003 Antisense
Sequence Source GenBank