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[171988_at] osteonectin

Description AV180507_rc /REP_DB=TREMBL Accession /GB=AV180507 /FEA=Transcript Cluster /DEF=Caenorhabditis elegans cDNA clone:yk608c8 : 3prime end, single read.
Cluster 6grp_highest_10_10 > 30      6grp_high1_10_10 > 05      4grp_highest_8_8 > 33      4grp_high_8_8 > 58      6grp_highest_nonorm > 05      6grp_total > 05      4grp_total > 61      6grp_highest_sub > De      6grp_cgc > 22      6grp_high1_sub > De      6grp_cgc_sub > De      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 7859
1 2 3 4 range
A 8844.8
(3)
P 		5628.1
(0.4)
P 		5571.7
(0.4)
P 		1318.5
(-3)
P 		7526
B 6597.2
(1.8)
P 		5844.3
(1.1)
P 		7780.4
(3)
P 		1609.8
(-3)
P 		6171
C 8820.7
(3)
P 		5622.2
(0.6)
P 		7224
(1.8)
P 		985.5
(-3)
P 		7835
avr 8087.6
(3) 		5698.2
(0.9) 		6858.7
(1.9) 		1304.6
(-3) 		6783

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 8685
1 2 3 4 5 6 range
A 4707.8
(1.2)
P 		5062.1
(1.9)
P 		5666.6
(3)
P 		4117.2
(0.1)
P 		4818.5
(1.4)
P 		2510.3
(-3)
P 		3156
B 11082.7
(3)
P 		6894.1
(-0.1)
P 		5268.6
(-1.3)
P 		2921.3
(-3)
P 		6214.2
(-0.6)
P 		3132.9
(-2.8)
P 		8161
C 10136.8
(3)
P 		5741.5
(-0.4)
P 		5391
(-0.7)
P 		5789.4
(-0.4)
P 		6632.3
(0.3)
P 		2397.5
(-3)
P 		7739
avr 8642.4
(3) 		5899.2
(0.2) 		5442.1
(-0.2) 		4276
(-1.4) 		5888.3
(0.2) 		2680.2
(-3) 		5962
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 3054
1 2 3 4 range
A 3220.4
(3)
P 		1221.7
(-1)
P 		1456.6
(-0.5)
P 		193
(-3)
P 		3027
B 2103.8
(3)
P 		1443.4
(0.7)
P 		1802.9
(2)
P 		343.1
(-3)
P 		1761
C 2919.9
(3)
P 		1616.4
(0.2)
P 		2517.8
(2.1)
P 		166.2
(-3)
P 		2754
avr 2748
(3) 		1427.2
(-0.2) 		1925.8
(1) 		234.1
(-3) 		2514

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 3245
1 2 3 4 5 6 range
A 867.8
(-1.7)
P 		1800.3
(3)
P 		1234.1
(0.1)
P 		1044.2
(-0.8)
P 		1454
(1.3)
P 		611.2
(-3)
P 		1189
B 3856.3
(3)
P 		2294.8
(-0)
P 		1279.4
(-2)
P 		773.8
(-3)
P 		2524.7
(0.4)
P 		1168.3
(-2.2)
P 		3083
C 3757.3
(3)
P 		1751.9
(-1.1)
P 		1372.1
(-1.8)
P 		1164.3
(-2.3)
P 		2146.1
(-0.3)
P 		802.6
(-3)
P 		2955
avr 2827.1
(3) 		1949
(0.3) 		1295.2
(-1.7) 		994.1
(-2.6) 		2041.6
(0.6) 		860.7
(-3) 		1966
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 171988_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.27878
Sequence Type Exemplar sequence
Representative Public ID AV180507 NCBI
Target Description AV180507_rc /REP_DB=TREMBL Accession /GB=AV180507 /FEA=Transcript Cluster /DEF=Caenorhabditis elegans cDNA clone:yk608c8 : 3prime end, single read.
Cluster Evidence This cluster is supported by a Transcript Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Genome Target Overlap based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade B annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_067638
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
GENEFINDER00000003003 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:1107449:1110567:1 None
SNAP00000002997 ENSEMBL cdna:SNAP chromosome:CEL140:IV:1108403:1110567:1 None
NM_067638 NCBI Caenorhabditis elegans C44B12.2 (osteonectin) mRNA, complete cds. A 
C44B12.2 ENSEMBL cdna:known chromosome:CEL140:IV:1107435:1110845:1 gene:C44B12.2 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:1110495-1110801(+) 68.51 68.51
Public Domain and Genome References
Gene Title osteonectin
Gene Symbol ost-1
Ensembl C44B12.2
Entrez Gene 176931 Entrez gene
SwissProt P34714 EMBL-EBI
WormBase CE08703 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE:1594354_AT similar to SPARC-like protein 1 precursor (High endothelial venule protein) (Hevin) (MAST 9) cfa
CANINE:1588998_AT similar to SPARC-like protein 1 precursor (High endothelial venule protein) (Hevin) (MAST 9) cfa
CANINE:1583269_S_AT similar to SPARC-like protein 1 precursor (High endothelial venule protein) (Hevin) (MAST 9) cfa
CANINE:1583268_AT similar to SPARC-like protein 1 precursor (High endothelial venule protein) (Hevin) (MAST 9) cfa
CANINE_2:CFA.3247.1.S1_AT similar to SPARC-like protein 1 precursor (High endothelial venule protein) (Hevin) (MAST 9) cfa
CANINE_2:CFAAFFX.15001.1.S1_S_AT similar to SPARC-like protein 1 precursor (High endothelial venule protein) (Hevin) (MAST 9) cfa
DROSOPHILA_2:1625825_AT dm
DROSGENOME1:144147_AT dm
CHICKEN:GGAAFFX.6912.1.S1_AT similar to hatching-suppressed osteonectin-like retinal protein precursor - Japanese quail gga
HG-U95AV2:36627_AT SPARC-like 1 (mast9, hevin) hs
HG-U133A:200795_AT SPARC-like 1 (mast9, hevin) hs
HG-U133_PLUS_2:200795_AT SPARC-like 1 (mast9, hevin) hs
HG-FOCUS:200795_AT SPARC-like 1 (mast9, hevin) hs
HG-U133A_2:200795_AT SPARC-like 1 (mast9, hevin) hs
HU35KSUBC:RC_AA069768_S_AT SPARC-like 1 (mast9, hevin) hs
HUGENEFL:X86693_AT SPARC-like 1 (mast9, hevin) hs
U133_X3P:G4758521_3P_AT SPARC-like 1 (mast9, hevin) hs
HG-U95C:57926_AT SPARC-like 1 (mast9, hevin) hs
HU35KSUBB:RC_AA045745_AT SPARC-like 1 (mast9, hevin) hs
MU11KSUBA:U64827_S_AT SPARC-like 1 (mast9, hevin) mm
MG-U74AV2:160319_AT SPARC-like 1 (mast9, hevin) mm
MG-U74CV2:171420_R_AT SPARC-like 1 (mast9, hevin) mm
MOE430A:1416114_AT SPARC-like 1 (mast9, hevin) mm
MOUSE430A_2:1416114_AT SPARC-like 1 (mast9, hevin) mm
MOUSE430_2:1416114_AT SPARC-like 1 (mast9, hevin) mm
RG-U34A:U27562_AT SPARC-like 1 (mast9, hevin) rn
RAE230A:1386865_AT SPARC-like 1 (mast9, hevin) rn
RAT230_2:1386865_AT SPARC-like 1 (mast9, hevin) rn
Gene Ontology
GO Molecular Function (view graph)
ID Description Evidence Links
5509 calcium ion binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast NP_500039 C44B12.2 [Caenorhabditis elegans] gb|AAB88325.1| Osteonectin (sparc) related protein 1 [Caenorhabditis elegans] sp|P34714|SPRC_CAEEL SPARC precursor (Secreted protein acidic and rich in cysteine) (Osteonectin) (ON) (Basement-membrane protein 40) (BM-40) gb|AAA16827.1| osteonectin 1.0E-140
blast CAE74733 Hypothetical protein CBG22551 [Caenorhabditis briggsae] 1.0E-138
blast NP_500039 C44B12.2 [Caenorhabditis elegans] gb|AAB88325.1| Osteonectin (sparc) related protein 1 [Caenorhabditis elegans] sp|P34714|SPRC_CAEEL SPARC precursor (Secreted protein acidic and rich in cysteine) (Osteonectin) (ON) (Basement-membrane protein 40) (BM-40) gb|AAA16827.1| osteonectin 1.0E-121
blast CAE74733 Hypothetical protein CBG22551 [Caenorhabditis briggsae] 1.0E-119
blast BAB20042.1 SPARC [Artemia franciscana] 6.0E-55
Sequence
Target Sequence 
>C. ELEGANS:171988_AT
gggattggggaaatgcttgggaattaaggngggggagatcccagggggatggtnnnnnnn
nnnnnnnnnnnnnntttnnnnnnnnnnnnnnnnnnnactttcnctccccaaattactaat
ccagcttgccgaactgccaacttgctcacatgctagaaatatctgtaaattacagattta
taggccatgaa

BLASTn GenBank NR
Note: "n"'s represent regions that are not probed by the probe sequences.
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GGGATTGGGGAAATGCTTGGGAATT 671 495 35 Antisense
CTCCCCAAATTACTAATCCAGCTTG 184 235 138 Antisense
CCCAAATTACTAATCCAGCTTGCCG 706 257 141 Antisense
AAATTACTAATCCAGCTTGCCGAAC 533 115 144 Antisense
TTACTAATCCAGCTTGCCGAACTGC 38 683 147 Antisense
CTGCCAACTTGCTCACATGCTAGAA 240 237 168 Antisense
GCCAACTTGCTCACATGCTAGAAAT 242 279 170 Antisense
AACTTGCTCACATGCTAGAAATATC 562 137 173 Antisense
CTTGCTCACATGCTAGAAATATCTG 234 219 175 Antisense
GCTCACATGCTAGAAATATCTGTAA 546 301 178 Antisense
AAATTACAGATTTATAGGCCATGAA 446 115 201 Antisense
Sequence Source Affymetrix Proprietary Database