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[171974_x_at]

Description AV182050_rc /REP_DB=TREMBL Accession /GB=AV182050 /FEA=Transcript Cluster /DEF=Caenorhabditis elegans cDNA clone:yk631d9 : 3prime end, single read.
Cluster 6grp_highest_10_10 > 80      6grp_high1_10_10 > 25      4grp_highest_8_8 > 57      4grp_high_8_8 > 41      6grp_highest_nonorm > 41      6grp_total > 05      4grp_total > 46      6grp_cgc > 03      6grp_high1_sub > 01_02      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 458
1 2 3 4 range
A 712.8
(3)
P 		546.9
(0.4)
P 		545.9
(0.4)
P 		334
(-3)
P 		379
B 678.9
(3)
P 		576.8
(1)
P 		375.7
(-3)
P 		525.9
(-0)
P 		303
C 792
(3)
P 		571.4
(-2.3)
P 		586.8
(-1.9)
P 		542.3
(-3)
P 		250
avr 727.9
(3) 		565
(-0.8) 		502.8
(-2.2) 		467.4
(-3) 		261

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 446
1 2 3 4 5 6 range
A 851.6
(3)
P 		684.3
(-0.6)
P 		570.1
(-3)
P 		600.1
(-2.4)
P 		717.1
(0.1)
P 		600.2
(-2.4)
P 		282
B 855.5
(3)
P 		852.7
(3)
P 		432.8
(-3)
P 		653.8
(0.1)
P 		649.3
(0.1)
P 		753.9
(1.6)
P 		423
C 731
(1.7)
P 		819.2
(3)
P 		628.1
(0.2)
P 		712.2
(1.4)
P 		581.7
(-0.5)
P 		409.9
(-3)
P 		409
avr 812.7
(3) 		785.4
(2.4) 		543.7
(-3) 		655.4
(-0.5) 		649.4
(-0.6) 		588
(-2) 		269
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 213
1 2 3 4 range
A 259.5
(3)
P 		118.7
(-1)
P 		142.7
(-0.3)
P 		48.9
(-3)
P 		211
B 216.5
(3)
P 		142.5
(-0.4)
P 		87
(-3)
P 		112.1
(-1.8)
P 		130
C 262.2
(3)
P 		164.3
(-0.4)
P 		204.5
(1)
P 		91.4
(-3)
P 		171
avr 246.1
(3) 		141.8
(-0.9) 		144.7
(-0.8) 		84.1
(-3) 		162

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 193
1 2 3 4 5 6 range
A 157
(-1.3)
P 		243.4
(3)
P 		124.2
(-3)
P 		152.2
(-1.6)
P 		216.4
(1.6)
P 		146.1
(-1.9)
P 		119
B 297.7
(3)
P 		283.8
(2.6)
P 		105.1
(-3)
P 		173.2
(-0.9)
P 		263.8
(1.9)
P 		281.1
(2.5)
P 		193
C 270.9
(3)
P 		250
(2.1)
P 		159.9
(-2)
P 		143.2
(-2.7)
P 		188.2
(-0.7)
P 		137.2
(-3)
P 		134
avr 241.9
(2.2) 		259.1
(3) 		129.7
(-3) 		156.2
(-1.8) 		222.8
(1.3) 		188.1
(-0.3) 		129
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 171974_x_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.28048
Sequence Type Exemplar sequence
Representative Public ID AV182050 NCBI
Target Description AV182050_rc /REP_DB=TREMBL Accession /GB=AV182050 /FEA=Transcript Cluster /DEF=Caenorhabditis elegans cDNA clone:yk631d9 : 3prime end, single read.
Cluster Evidence This cluster is supported by a Transcript Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Genome Target Overlap based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade B annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_062939
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_062939 NCBI Caenorhabditis elegans DNaJ domain (prokaryotic heat shock protein) family member (dnj-8) (dnj-8) mRNA, complete cds. None
C56C10.13 ENSEMBL cdna:known chromosome:CEL140:II:6594335:6598015:-1 gene:C56C10.13 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:6594381-6594668(-) 74.28 79.42
Public Domain and Genome References
Gene Title
Gene Symbol dnj-8
Ensembl C56C10.13
Entrez Gene 174093 Entrez gene
SwissProt Q95QQ1 EMBL-EBI
WormBase CE28901 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE_2:CFA.18042.1.S1_S_AT similar to DnaJ (Hsp40) homolog, subfamily C, member 16 cfa
CANINE_2:CFAAFFX.24923.1.S1_S_AT similar to DnaJ (Hsp40) homolog, subfamily C, member 16 cfa
CHICKEN:GGAAFFX.12043.1.S1_S_AT similar to KIAA0962 protein gga
HG-U133_PLUS_2:217358_AT DnaJ (Hsp40) homolog, subfamily C, member 16 hs
HG-U133A_2:217358_AT DnaJ (Hsp40) homolog, subfamily C, member 16 hs
HG-U133A:217358_AT DnaJ (Hsp40) homolog, subfamily C, member 16 hs
HG-U133_PLUS_2:212911_AT DnaJ (Hsp40) homolog, subfamily C, member 16 hs
HG-U133A_2:212911_AT DnaJ (Hsp40) homolog, subfamily C, member 16 hs
HG-U133A:212911_AT DnaJ (Hsp40) homolog, subfamily C, member 16 hs
HU35KSUBA:RC_AA258203_AT DnaJ (Hsp40) homolog, subfamily C, member 16 hs
HU35KSUBC:RC_H80637_AT DnaJ (Hsp40) homolog, subfamily C, member 16 hs
U133_X3P:HS.9059.0.S2_3P_AT DnaJ (Hsp40) homolog, subfamily C, member 16 hs
U133_X3P:HS.9059.0.S1_3P_AT DnaJ (Hsp40) homolog, subfamily C, member 16 hs
U133_X3P:HS.272327.0.S1_3P_AT DnaJ (Hsp40) homolog, subfamily C, member 16 hs
HG-U133A_2:212908_AT DnaJ (Hsp40) homolog, subfamily C, member 16 hs
HG-U133_PLUS_2:212908_AT DnaJ (Hsp40) homolog, subfamily C, member 16 hs
HG-U133A:212908_AT DnaJ (Hsp40) homolog, subfamily C, member 16 hs
HG-U95AV2:33170_AT DnaJ (Hsp40) homolog, subfamily C, member 16 hs
HG-U133_PLUS_2:242947_AT DnaJ (Hsp40) homolog, subfamily C, member 16 hs
HG-U133B:242947_AT DnaJ (Hsp40) homolog, subfamily C, member 16 hs
HG-U95D:91976_AT DnaJ (Hsp40) homolog, subfamily C, member 16 hs
HU35KSUBC:RC_D11881_AT DnaJ (Hsp40) homolog, subfamily C, member 16 hs
MG-U74BV2:105676_AT DnaJ (Hsp40) homolog, subfamily C, member 16 mm
MOE430B:1436209_AT DnaJ (Hsp40) homolog, subfamily C, member 16 mm
MOUSE430_2:1436209_AT DnaJ (Hsp40) homolog, subfamily C, member 16 mm
MU19KSUBC:TC39745_AT MKIAA0962 protein mm
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6457 protein folding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAK93843 Dnaj domain (prokaryotic heat shock protein) protein 8 [Caenorhabditis elegans] ref|NP_495340.1| DNaJ domain (prokaryotic heat shock protein) family member (dnj-8) [Caenorhabditis elegans] 0.0
blast T15851 hypothetical protein C56C10.11 - Caenorhabditis elegans 0.0
blast CAE60663 Hypothetical protein CBG04316 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
scop a.2.3.Chaperone J-domain All alpha proteins; Long alpha-hairpin; Chaperone J-domain; Chaperone J-domain 4.00000007816593E-24
Pfam IPR001623 EMBL-EBI Heat shock protein DnaJ, N-terminal 1.6E-32
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_495340.1 1 526-548
C56C10.13 1 526-548
Sequence
Target Sequence 
>C. ELEGANS:171974_X_AT
attccccgggtggccggataatctgaagtaatctttcaactaattaatgaggtttnnnnn
ngttcatcaatatgtctaccttctcgtcctctctttntnnnnnnnnnnnnnnnnnnnnnn
nnnnnnnacatttggtgctcaaagattttcttctcagctagttcacaagttcacaactct
tgatcttnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnctcttattgcttcagttatc
taacttgttta

BLASTn GenBank NR
Note: "n"'s represent regions that are not probed by the probe sequences.
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATTCCCCGGGTGGCCGGATAATCTG 637 5 22 Antisense
GGTGGCCGGATAATCTGAAGTAATC 381 499 30 Antisense
GAAGTAATCTTTCAACTAATTAATG 207 335 46 Antisense
TCATCAATATGTCTACCTTCTCGTC 579 619 85 Antisense
GGTGCTCAAAGATTTTCTTCTCAGC 492 501 155 Antisense
GATTTTCTTCTCAGCTAGTTCACAA 382 429 165 Antisense
TTCTCAGCTAGTTCACAAGTTCACA 4 691 172 Antisense
GCTAGTTCACAAGTTCACAACTCTT 203 303 178 Antisense
TCACAAGTTCACAACTCTTGATCTT 187 629 184 Antisense
CTCTTATTGCTTCAGTTATCTAACT 127 231 242 Antisense
TTGCTTCAGTTATCTAACTTGTTTA 499 699 248 Antisense
Sequence Source Affymetrix Proprietary Database