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[171972_x_at]

Description AV177526_rc /REP_DB=TREMBL Accession /GB=AV177526 /FEA=Transcript Cluster /DEF=Caenorhabditis elegans cDNA clone:yk529h4 : 3prime end, single read.
Cluster 6grp_highest_10_10 > 60      6grp_high1_10_10 > 17      4grp_highest_8_8 > 46      4grp_high_8_8 > 11      6grp_highest_nonorm > 80      6grp_total > 17      4grp_total > 21      6grp_highest_sub > De      6grp_cgc > 13      6grp_high1_sub > De      6grp_cgc_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 276
1 2 3 4 range
A 305
(1.7)
P 		191.5
(-1.2)
P 		120.3
(-3)
P 		357.5
(3)
P 		237
B 308.4
(0.5)
P 		226.9
(-1.8)
P 		185.8
(-3)
P 		395.9
(3)
P 		210
C 326.9
(3)
P 		210.1
(-1.9)
P 		182.9
(-3)
P 		327.1
(3)
P 		144
avr 313.4
(1.6) 		209.5
(-1.6) 		163
(-3) 		360.2
(3) 		197

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 287
1 2 3 4 5 6 range
A 349.2
(3)
P 		317.1
(2.1)
P 		153.3
(-2.4)
P 		132
(-3)
P 		140.3
(-2.8)
P 		176.7
(-1.8)
P 		217
B 334.5
(3)
P 		235.5
(-0.5)
P 		165.6
(-3)
P 		189.4
(-2.2)
P 		175.4
(-2.7)
P 		166.9
(-3)
P 		169
C 419.4
(3)
P 		225.7
(-2.3)
P 		221.2
(-2.4)
P 		230.3
(-2.2)
P 		205.5
(-2.9)
P 		200.4
(-3)
P 		219
avr 367.7
(3) 		259.4
(-0.3) 		180
(-2.8) 		183.9
(-2.7) 		173.7
(-3) 		181.3
(-2.8) 		194
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 80
1 2 3 4 range
A 111.1
(3)
P 		41.6
(-2.2)
P 		31.5
(-3)
P 		52.3
(-1.4)
P 		80
B 98.3
(3)
P 		56
(-1.6)
P 		43.1
(-3)
P 		84.4
(1.5)
P 		55
C 108.2
(3)
P 		60.4
(-2.4)
P 		63.8
(-2)
P 		55.1
(-3)
P 		53
avr 105.9
(3) 		52.7
(-2.3) 		46.1
(-3) 		63.9
(-1.2) 		60

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 122
1 2 3 4 5 6 range
A 64.4
(-0.7)
P 		112.8
(3)
P 		33.4
(-3)
P 		33.5
(-3)
P 		42.3
(-2.3)
P 		43
(-2.3)
P 		79
B 116.4
(3)
P 		78.4
(0)
P 		40.2
(-3)
P 		50.2
(-2.2)
P 		71.3
(-0.6)
P 		62.2
(-1.3)
P 		76
C 155.5
(3)
P 		68.9
(-1.8)
P 		56.3
(-2.5)
P 		46.3
(-3)
P 		66.5
(-1.9)
P 		67.1
(-1.9)
P 		109
avr 112.1
(3) 		86.7
(0.8) 		43.3
(-3) 		43.3
(-3) 		60
(-1.5) 		57.4
(-1.8) 		69
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 171972_x_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.28001
Sequence Type Exemplar sequence
Representative Public ID AV177526 NCBI
Target Description AV177526_rc /REP_DB=TREMBL Accession /GB=AV177526 /FEA=Transcript Cluster /DEF=Caenorhabditis elegans cDNA clone:yk529h4 : 3prime end, single read.
Cluster Evidence This cluster is supported by a Transcript Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_064777(10)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_064777 NCBI Caenorhabditis elegans Kinesin-Like Protein family member (klp-20) (klp-20) mRNA, complete cds. 10/11 A 
Y50D7A.6 ENSEMBL cdna:known chromosome:CEL140:III:221418:228837:1 gene:Y50D7A.6 10/11 A 
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
GENEFINDER00000021197 5/11 Cross Hyb Matching Probes None
SNAP00000021185 3/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:226966-228742(+) 97.36 97.36
Public Domain and Genome References
Gene Title
Gene Symbol klp-20
Ensembl Y50D7A.6
Entrez Gene 3565057 Entrez gene
SwissProt Q965T6 EMBL-EBI
WormBase CE26143 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE_2:CFAAFFX.2266.1.S1_S_AT similar to Kinesin-like protein KIF3A (Microtubule plus end-directed kinesin motor 3A) cfa
DROSOPHILA_2:1628601_AT Kinesin-like protein at 64D dm
DROSGENOME1:143453_AT Kinesin-like protein at 64D dm
CHICKEN:GGAAFFX.4247.1.A1_AT kinesin family member 3A gga
HU35KSUBC:RC_W70205_AT kinesin family member 3A hs
U133_X3P:HS.43670.0.S1_3P_AT kinesin family member 3A hs
HG-FOCUS:213623_AT kinesin family member 3A hs
HG-U133A_2:213623_AT kinesin family member 3A hs
HG-U133_PLUS_2:213623_AT kinesin family member 3A hs
HG-U133A:213623_AT kinesin family member 3A hs
HG-U95AV2:39956_AT kinesin family member 3A hs
HG-U95D:85549_AT kinesin family member 3A hs
MOUSE430_2:1420375_AT kinesin family member 3A mm
MOE430A:1420375_AT kinesin family member 3A mm
MOUSE430A_2:1420375_AT kinesin family member 3A mm
MG-U74AV2:100398_AT kinesin family member 3A mm
MU11KSUBA:D12645_S_AT kinesin family member 3A mm
MG-U74BV2:115929_AT kinesin family member 3A mm
MG-U74AV2:161275_AT kinesin family member 3A mm
MOE430A:1449841_AT kinesin family member 3A mm
MOUSE430A_2:1449841_AT kinesin family member 3A mm
MOUSE430_2:1449841_AT kinesin family member 3A mm
MG-U74CV2:130184_AT Kinesin family member 3A (Kif3a), mRNA mm
MU19KSUBB:TC26270_S_AT Kinesin family member 3A (Kif3a), mRNA mm
Gene Ontology
GO Cellular Component (view graph)
ID Description Evidence Links
5875 microtubule associated complex inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
3774 motor activity inferred from electronic annotation QuickGO AmiGO
5524 ATP binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAK68513 Kinesin-like protein protein 20 [Caenorhabditis elegans] ref|NP_497178.1| Kinesin-Like Protein family member (klp-20) [Caenorhabditis elegans] 0.0
blast AAF99087 KRP85 [Caenorhabditis elegans] 0.0
blast CAE69512 Hypothetical protein CBG15720 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR001752 EMBL-EBI Kinesin, motor region 1.0E-126
Sequence
Target Sequence 
>C. ELEGANS:171972_X_AT
ttccgcgccgccggttaagaaggagttttcgaacaacaaccaaacggtgccaatgtacta
ctcgtatcgtgcagatcttggtgcatcaaccgccgagcacaggccacggacgagctccnn
nnnncatcgagcatcaattcgactacaacaattgctcacataactggtacaggtacatat
tcatctccatcaatgttacgctgaatttgcagtgacatcttttggctcc

BLASTn GenBank NR
Note: "n"'s represent regions that are not probed by the probe sequences.
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TTCCGCGCCGCCGGTTAAGAAGGAG 26 659 17 Antisense
CCAAACGGTGCCAATGTACTACTCG 448 273 56 Antisense
GTACTACTCGTATCGTGCAGATCTT 643 459 71 Antisense
TGCAGATCTTGGTGCATCAACCGCC 213 579 86 Antisense
CATCGAGCATCAATTCGACTACAAC 39 213 141 Antisense
ATTCGACTACAACAATTGCTCACAT 508 9 153 Antisense
ATTGCTCACATAACTGGTACAGGTA 586 3 167 Antisense
GGTACAGGTACATATTCATCTCCAT 708 503 182 Antisense
ATCTCCATCAATGTTACGCTGAATT 6 33 199 Antisense
GTTACGCTGAATTTGCAGTGACATC 427 445 211 Antisense
ATTTGCAGTGACATCTTTTGGCTCC 664 17 221 Antisense
Sequence Source Affymetrix Proprietary Database