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[171955_x_at]

Description AV200306_rc /REP_DB=TREMBL Accession /GB=AV200306 /FEA=Transcript Cluster /DEF=Caenorhabditis elegans cDNA clone:yk560c9 : 3prime end, single read.
Cluster 6grp_highest_10_10 > 30      6grp_high1_10_10 > 04      4grp_highest_8_8 > 36      4grp_high_8_8 > 0      6grp_highest_nonorm > 17      6grp_total > 22      4grp_total > 07      6grp_highest_sub > De      6grp_cgc > 42      6grp_high1_sub > De      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 9253
1 2 3 4 range
A 17080.9
(1.4)
P 		10509.3
(-3)
P 		16591.5
(1.1)
P 		19435.5
(3)
P 		8926
B 17484.6
(3)
P 		10183
(-3)
P 		11994.4
(-1.5)
P 		12222
(-1.3)
P 		7302
C 14302.8
(1.4)
P 		12873.1
(-3)
P 		14009.4
(0.5)
P 		14809.4
(3)
P 		1936
avr 16289.4
(3) 		11188.5
(-3) 		14198.4
(0.5) 		15489
(2.1) 		5101

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 10761
1 2 3 4 5 6 range
A 8583.4
(-2.8)
P 		8415.4
(-3)
P 		13545.4
(3)
P 		9491.2
(-1.7)
P 		11304.4
(0.4)
P 		9898.9
(-1.3)
P 		5130
B 15994.6
(3)
P 		14417.4
(1.6)
P 		11146.3
(-1.4)
P 		11330.5
(-1.3)
P 		10355.1
(-2.2)
P 		9426.6
(-3)
P 		6568
C 14858
(3)
P 		14677.2
(2.9)
P 		11349.4
(0.8)
P 		10119.5
(0)
P 		12783.9
(1.7)
P 		5233.3
(-3)
P 		9625
avr 13145.3
(3) 		12503.3
(2.2) 		12013.7
(1.6) 		10313.7
(-0.4) 		11481.1
(1) 		8186.3
(-3) 		4959
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 3938
1 2 3 4 range
A 6219.1
(3)
P 		2281.3
(-3)
P 		4337.3
(0.1)
P 		2845
(-2.1)
P 		3938
B 5575.7
(3)
P 		2515
(-3)
P 		2779.4
(-2.5)
P 		2604.5
(-2.8)
P 		3061
C 4734.6
(2.6)
P 		3701.1
(0)
P 		4882.7
(3)
P 		2497.1
(-3)
P 		2386
avr 5509.8
(3) 		2832.5
(-2.6) 		3999.8
(-0.2) 		2648.9
(-3) 		2861

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 3983
1 2 3 4 5 6 range
A 1582.3
(-3)
P 		2992.9
(1.6)
P 		2949.9
(1.5)
P 		2407.2
(-0.3)
P 		3411.2
(3)
P 		2410.1
(-0.3)
P 		1829
B 5565.4
(3)
P 		4798.9
(1.4)
P 		2706.7
(-3)
P 		3001.1
(-2.4)
P 		4207.1
(0.1)
P 		3515.1
(-1.3)
P 		2859
C 5507.2
(3)
P 		4478.5
(1.4)
P 		2888.6
(-1.2)
P 		2035
(-2.5)
P 		4136.7
(0.8)
P 		1751.9
(-3)
P 		3755
avr 4218.3
(3) 		4090.1
(2.6) 		2848.4
(-1.7) 		2481.1
(-3) 		3918.3
(2) 		2559
(-2.7) 		1737
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 171955_x_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.27779
Sequence Type Exemplar sequence
Representative Public ID AV200306 NCBI
Target Description AV200306_rc /REP_DB=TREMBL Accession /GB=AV200306 /FEA=Transcript Cluster /DEF=Caenorhabditis elegans cDNA clone:yk560c9 : 3prime end, single read.
Cluster Evidence This cluster is supported by a Transcript Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_077538(10), NM_077537(10)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_077538 NCBI Caenorhabditis elegans Fourteen-Three-Three family member (ftt-2) (ftt-2) mRNA, complete cds. 10/11 None
NM_077537 NCBI Caenorhabditis elegans Fourteen-Three-Three family member (ftt-2) (ftt-2) mRNA, complete cds. 10/11 None
F52D10.3a.1 ENSEMBL cdna:known chromosome:CEL140:X:11603632:11605303:-1 gene:F52D10.3 10/11 A 
F52D10.3b.1 ENSEMBL cdna:known chromosome:CEL140:X:11603635:11605333:-1 gene:F52D10.3 10/11 A 
F52D10.3b.2 ENSEMBL cdna:known chromosome:CEL140:X:11603635:11605342:-1 gene:F52D10.3 10/11 A 
F52D10.3b.3 ENSEMBL cdna:known chromosome:CEL140:X:11603635:11605690:-1 gene:F52D10.3 10/11 A 
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
F52D10.3a.2 2/11 Cross Hyb Matching Probes A 
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:11603706-11603925(-) 72.28 72.28
Public Domain and Genome References
Ensembl F52D10.3
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA91474 Hypothetical protein F52D10.3a [Caenorhabditis elegans] ref|NP_509939.1| Fourteen-Three-Three family member (ftt-2) [Caenorhabditis elegans] sp|Q20655|14332_CAEEL 14-3-3-like protein 1.0E-129
blast CAE70609 Hypothetical protein CBG17289 [Caenorhabditis briggsae] 1.0E-128
blast AAR85527 14-3-3b protein [Meloidogyne incognita] 1.0E-118
blast CAC42300 Hypothetical protein F52D10.3b [Caenorhabditis elegans] ref|NP_509938.2| Fourteen-Three-Three family member (ftt-2) [Caenorhabditis elegans] 1.0E-107
Protein Domains
Database ID Description E-Value
Pfam IPR000308 EMBL-EBI 14-3-3 protein 1.0E-126
Pfam IPR000308 EMBL-EBI 14-3-3 protein 2.0E-115
Sequence
Target Sequence 
>C. ELEGANS:171955_X_AT
ccttcacttggttttggaaaatgataaataatactatttgtcatatgatttatttattac
cattgactagttaaannnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnntcact
ccccttttcccatcaaccaaatgcagaagttattcaaatttctgtgctgagcccactcat
caaaacttcccgtctctacccatacaattcaatg

BLASTn GenBank NR
Note: "n"'s represent regions that are not probed by the probe sequences.
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CCTTCACTTGGTTTTGGAAAATGAT 383 267 71 Antisense
ATAATACTATTTGTCATATGATTTA 564 25 98 Antisense
TGATTTATTTATTACCATTGACTAG 384 563 116 Antisense
CCCTTTTCCCATCAACCAAATGCAG 542 259 192 Antisense
TTTCCCATCAACCAAATGCAGAAGT 121 669 196 Antisense
TCAACCAAATGCAGAAGTTATTCAA 476 627 203 Antisense
GTTATTCAAATTTCTGTGCTGAGCC 3 449 219 Antisense
TCAAATTTCTGTGCTGAGCCCACTC 359 629 224 Antisense
TGTGCTGAGCCCACTCATCAAAACT 355 557 233 Antisense
TGCTGAGCCCACTCATCAAAACTTC 201 583 235 Antisense
CCGTCTCTACCCATACAATTCAATG 325 265 260 Antisense
Sequence Source Affymetrix Proprietary Database