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[171926_x_at] alcohol dehydrogenase; non-specific lipid transfer protein

Description CEK062EYR_rc /REP_DB=TREMBL Accession /GB=D71126 /FEA=Transcript Cluster /DEF=C.elegans cDNA clone yk62e11 : 3prime end, single read.
Cluster 6grp_total > 37      4grp_total > 09      6grp_cgc > 15      6grp_cgc_sub > Cu      6grp_total_sub > Cup      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1000
1 2 3 4 range
A 308.8
(-2.5)
A 		405
(-1.9)
A 		219.4
(-3)
P 		1219.5
(3)
P 		1000
B 438.7
(-3)
P 		493.5
(-2.3)
A 		732.7
(0.6)
P 		930.7
(3)
A 		492
C 486.6
(-3)
A 		493.3
(-2.9)
A 		593.6
(-1.5)
P 		929.3
(3)
P 		443
avr 411.4
(-3) 		463.9
(-2.5) 		515.2
(-2) 		1026.5
(3) 		615

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1153
1 2 3 4 5 6 range
A 515.3
(-0)
P 		586.2
(1)
A 		716.9
(3)
P 		317.2
(-3)
P 		553.2
(0.5)
P 		641
(1.9)
A 		400
B 1277
(3)
A 		562.7
(-1.6)
M 		716.4
(-0.6)
A 		605.6
(-1.3)
P 		347.2
(-3)
P 		718.2
(-0.6)
P 		930
C 853.4
(3)
A 		839.8
(2.9)
M 		188.8
(-2.5)
P 		573.6
(0.7)
P 		124.2
(-3)
P 		662.9
(1.4)
P 		729
avr 881.9
(3) 		662.9
(0.6) 		540.7
(-0.8) 		498.8
(-1.3) 		341.5
(-3) 		674
(0.7) 		540
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 150
1 2 3 4 range
A 112.4
(-0.3)
A 		87.9
(-1.5)
A 		57.4
(-3)
P 		178.5
(3)
P 		121
B 139.9
(-1.6)
P 		121.9
(-3)
A 		169.8
(0.8)
P 		198.3
(3)
A 		76
C 161.1
(-1.2)
A 		141.8
(-3)
A 		206.9
(3)
P 		156.7
(-1.6)
P 		65
avr 137.8
(-1) 		117.2
(-3) 		144.7
(-0.3) 		177.8
(3) 		61

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 404
1 2 3 4 5 6 range
A 95
(-2.3)
P 		208.5
(3)
A 		156.1
(0.5)
P 		80.5
(-3)
P 		166.9
(1.1)
P 		156.1
(0.5)
A 		128
B 444.3
(3)
A 		187.3
(-2.1)
M 		174
(-2.3)
A 		160.4
(-2.6)
P 		141.1
(-3)
P 		267.8
(-0.5)
P 		303
C 316.3
(3)
A 		256.3
(1.7)
M 		48.1
(-2.8)
P 		115.3
(-1.4)
P 		40.2
(-3)
P 		221.9
(0.9)
P 		276
avr 285.2
(3) 		217.4
(0.6) 		126.1
(-2.6) 		118.7
(-2.9) 		116.1
(-3) 		215.3
(0.5) 		169
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 171926_x_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.27704
Sequence Type Exemplar sequence
Representative Public ID CEK062EYR NCBI
Target Description CEK062EYR_rc /REP_DB=TREMBL Accession /GB=D71126 /FEA=Transcript Cluster /DEF=C.elegans cDNA clone yk62e11 : 3prime end, single read.
Cluster Evidence This cluster is supported by a Transcript Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Genome Target Overlap based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade B annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_076745
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
M03A8.1 ENSEMBL cdna:known chromosome:CEL140:X:6818391:6820015:1 gene:M03A8.1 A 
GENEFINDER00000018347 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:6818699:6819942:1 None
SNAP00000018337 ENSEMBL cdna:SNAP chromosome:CEL140:X:6818721:6819942:1 None
NM_076745 NCBI Caenorhabditis elegans DeHydrogenases, Short chain family member (dhs-28) (dhs-28) mRNA, complete cds. A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:6819663-6820019(+) 84.2 84.2
Public Domain and Genome References
Gene Title alcohol dehydrogenase; non-specific lipid transfer protein
Gene Symbol dhs-28
Ensembl M03A8.1
Entrez Gene 180950 Entrez gene
SwissProt Q21481 EMBL-EBI
WormBase CE04770 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE:1604291_AT similar to Peroxisomal multifunctional enzyme type 2 (MFE-2) (D-bifunctional protein) (DBP) (17-beta-hydroxysteroid dehydrogenase 4) (17-beta-HSD 4) cfa
CANINE:1597302_AT similar to Peroxisomal multifunctional enzyme type 2 (MFE-2) (D-bifunctional protein) (DBP) (17-beta-hydroxysteroid dehydrogenase 4) (17-beta-HSD 4) cfa
CANINE_2:CFA.14480.1.S1_AT similar to Peroxisomal multifunctional enzyme type 2 (MFE-2) (D-bifunctional protein) (DBP) (17-beta-hydroxysteroid dehydrogenase 4) (17-beta-HSD 4) cfa
CANINE_2:CFA.18106.1.S1_S_AT similar to Peroxisomal multifunctional enzyme type 2 (MFE-2) (D-bifunctional protein) (DBP) (17-beta-hydroxysteroid dehydrogenase 4) (17-beta-HSD 4) cfa
CANINE_2:CFAAFFX.1219.1.S1_S_AT similar to Peroxisomal multifunctional enzyme type 2 (MFE-2) (D-bifunctional protein) (DBP) (17-beta-hydroxysteroid dehydrogenase 4) (17-beta-HSD 4) cfa
DROSOPHILA_2:1638695_AT dm
DROSGENOME1:152603_AT dm
CHICKEN:GGA.3546.1.S1_AT hydroxysteroid (17-beta) dehydrogenase 4 gga
HG-U133A:201413_AT hydroxysteroid (17-beta) dehydrogenase 4 hs
HG-U133_PLUS_2:201413_AT hydroxysteroid (17-beta) dehydrogenase 4 hs
HG-U133A_2:201413_AT hydroxysteroid (17-beta) dehydrogenase 4 hs
HG-FOCUS:201413_AT hydroxysteroid (17-beta) dehydrogenase 4 hs
HUGENEFL:X87176_AT hydroxysteroid (17-beta) dehydrogenase 4 hs
U133_X3P:G4504504_3P_AT hydroxysteroid (17-beta) dehydrogenase 4 hs
U133_X3P:HS2.356894.1.S1_3P_S_AT hydroxysteroid (17-beta) dehydrogenase 4 hs
HG-U95AV2:36626_AT hydroxysteroid (17-beta) dehydrogenase 4 hs
U133_X3P:HS2.356894.1.S1_3P_X_AT Hydroxysteroid (17-beta) dehydrogenase 4 hs
U133_X3P:HS2.356894.1.S1_3P_AT Hydroxysteroid (17-beta) dehydrogenase 4 hs
HG-U133_PLUS_2:1563696_AT Hydroxysteroid (17-beta) dehydrogenase 4 hs
MOUSE430A_2:1455777_X_AT hydroxysteroid (17-beta) dehydrogenase 4 mm
MOE430A:1455777_X_AT hydroxysteroid (17-beta) dehydrogenase 4 mm
MOUSE430_2:1455777_X_AT hydroxysteroid (17-beta) dehydrogenase 4 mm
MU11KSUBB:X89998_S_AT hydroxysteroid (17-beta) dehydrogenase 4 mm
MG-U74AV2:97515_AT hydroxysteroid (17-beta) dehydrogenase 4 mm
MOE430A:1417369_AT hydroxysteroid (17-beta) dehydrogenase 4 mm
MOUSE430A_2:1417369_AT hydroxysteroid (17-beta) dehydrogenase 4 mm
MOUSE430_2:1417369_AT hydroxysteroid (17-beta) dehydrogenase 4 mm
MU19KSUBB:TC31197_AT Hydroxysteroid (17-beta) dehydrogenase 4, mRNA (cDNA clone MGC:13992 IMAGE:4209394) mm
MU19KSUBB:TC33135_I_AT Hydroxysteroid (17-beta) dehydrogenase 4, mRNA (cDNA clone MGC:13992 IMAGE:4209394) mm
RT-U34:RC_AI013834_S_AT hydroxysteroid (17-beta) dehydrogenase 4 rn
RG-U34A:RC_AI013834_S_AT hydroxysteroid (17-beta) dehydrogenase 4 rn
RT-U34:S83279_AT hydroxysteroid (17-beta) dehydrogenase 4 rn
RG-U34A:S83279_AT hydroxysteroid (17-beta) dehydrogenase 4 rn
RT-U34:RC_AI101743_S_AT hydroxysteroid (17-beta) dehydrogenase 4 rn
RG-U34A:RC_AI101743_S_AT hydroxysteroid (17-beta) dehydrogenase 4 rn
RT-U34:S83279_G_AT hydroxysteroid (17-beta) dehydrogenase 4 rn
RG-U34A:S83279_G_AT hydroxysteroid (17-beta) dehydrogenase 4 rn
RAE230A:1367672_AT hydroxysteroid (17-beta) dehydrogenase 4 rn
RAT230_2:1367672_AT hydroxysteroid (17-beta) dehydrogenase 4 rn
YEAST_2:1769308_AT Multifunctional enzyme of the peroxisomal fatty acid beta-oxidation pathway; has 3-hydroxyacyl-CoA dehydrogenase and enoyl-CoA hydratase activities Sc
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
8152 metabolism inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
5498 sterol carrier activity inferred from electronic annotation QuickGO AmiGO
16491 oxidoreductase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAA83184 Dehydrogenases, short chain protein 28 [Caenorhabditis elegans] ref|NP_509146.1| DeHydrogenases, Short chain family member (dhs-28) [Caenorhabditis elegans] 0.0
blast CAE65794 Hypothetical protein CBG10899 [Caenorhabditis briggsae] 0.0
blast CAE65794 Hypothetical protein CBG10899 [Caenorhabditis briggsae] 1.0E-166
blast XP_635235 hypothetical protein DDB0201628 [Dictyostelium discoideum] dbj|BAA94961.1| peroxisomal multifunctional enzyme MFE homolog [Dictyostelium discoideum] gb|EAL61607.1| hypothetical protein DDB0201628 [Dictyostelium discoideum] 1.0E-109
Protein Domains
Database ID Description E-Value
Pfam IPR002198 EMBL-EBI Short-chain dehydrogenase/reductase SDR 6.3E-54
Pfam IPR003033 EMBL-EBI Sterol-binding 9.4E-44
Pfam IPR002198 EMBL-EBI Short-chain dehydrogenase/reductase SDR 3.1E-32
Pfam IPR003033 EMBL-EBI Sterol-binding 9.4E-44
Pfam IPR002198 EMBL-EBI Short-chain dehydrogenase/reductase SDR 4.8E-31
Pfam IPR003033 EMBL-EBI Sterol-binding 9.4E-44
Sequence
Target Sequence 
>C. ELEGANS:171926_X_AT
aggacgagcttgggaaattcacttcttgactttcaaaagggcttctccttccgtgtactt
gggtgatgtaaaggagcggggagaaagctaacgcggacggtcactgttgctgatagcgat
tttgttgacattgcagccggaaaattgaatgctcacaaggcatttcatgagcggaaaact
gaaagtgaagggaaacgtaatgcttcttcagaaacttcaaaccgtt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AGGACGAGCTTGGGAAATTCACTTC 254 55 28 Antisense
GGGAAATTCACTTCTTGACTTTCAA 568 493 39 Antisense
TTTCAAAAGGGCTTCTCCTTCCGTG 107 667 58 Antisense
TCTCCTTCCGTGTACTTGGGTGATG 80 609 71 Antisense
GGGTGATGTAAAGGAGCGGGGAGAA 548 495 88 Antisense
GAAAGCTAACGCGGACGGTCACTGT 75 359 110 Antisense
GACGGTCACTGTTGCTGATAGCGAT 417 377 123 Antisense
GCGATTTTGTTGACATTGCAGCCGG 432 291 143 Antisense
GAATGCTCACAAGGCATTTCATGAG 284 331 174 Antisense
AAGGGAAACGTAATGCTTCTTCAGA 606 159 215 Antisense
GCTTCTTCAGAAACTTCAAACCGTT 478 305 229 Antisense
Sequence Source Affymetrix Proprietary Database