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[171903_x_at]

Description AV199932_rc /REP_DB=TREMBL Accession /GB=AV199932 /FEA=Transcript Cluster /DEF=Caenorhabditis elegans cDNA clone:yk553g4 : 3prime end, single read.
Cluster 6grp_total > 97      4grp_total > 32      6grp_total_sub > Increase      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 58
1 2 3 4 range
A 9.2
(-3)
A 		32.6
(-0.5)
P 		35.3
(-0.2)
A 		64.6
(3)
A 		55
B 34.4
(1.2)
A 		8.9
(-3)
A 		45.1
(3)
A 		44.1
(2.8)
A 		36
C 14
(-1.7)
A 		42.3
(3)
A 		41.6
(2.9)
P 		6.5
(-3)
A 		36
avr 19.2
(-3) 		27.9
(-0.6) 		40.7
(3) 		38.4
(2.4) 		22

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 56
1 2 3 4 5 6 range
A 3.8
(-2.6)
A 		2.2
(-2.9)
A 		1.7
(-3)
A 		28.6
(1.6)
A 		24.9
(1)
A 		36.9
(3)
A 		35
B 2.3
(-3)
A 		8.8
(-2.2)
A 		26.7
(-0.3)
A 		1.9
(-3)
A 		57
(3)
P 		42.9
(1.5)
A 		55
C 1.4
(-3)
A 		4
(-2.7)
A 		26
(-0)
A 		15.3
(-1.3)
A 		10.3
(-1.9)
A 		51
(3)
A 		50
avr 2.5
(-3) 		5
(-2.6) 		18.1
(-0.7) 		15.3
(-1.1) 		30.7
(1.1) 		43.6
(3) 		41
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 13
1 2 3 4 range
A 3.4
(-3)
A 		7.1
(0.6)
P 		9.2
(2.7)
A 		9.5
(3)
A 		6
B 11
(3)
A 		2.2
(-3)
A 		10.5
(2.7)
A 		9.4
(1.9)
A 		9
C 4.6
(-1.4)
A 		12.2
(2)
A 		14.5
(3)
P 		1.1
(-3)
A 		13
avr 6.3
(-3) 		7.2
(-1.9) 		11.4
(3) 		6.7
(-2.5) 		5

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 23
1 2 3 4 5 6 range
A 0.7
(-2.8)
A 		0.8
(-2.7)
A 		0.4
(-3)
A 		7.2
(1.7)
A 		7.5
(2)
A 		9
(3)
A 		9
B 0.8
(-2.9)
A 		2.9
(-2.4)
A 		6.5
(-1.4)
A 		0.5
(-3)
A 		23.2
(3)
P 		16
(1.1)
A 		23
C 0.5
(-3)
A 		1.2
(-2.7)
A 		6.6
(-0.8)
A 		3.1
(-2.1)
A 		3.3
(-2)
A 		17.1
(3)
A 		17
avr 0.7
(-3) 		1.6
(-2.6) 		4.5
(-1.3) 		3.6
(-1.7) 		11.3
(1.8) 		14
(3) 		13
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 171903_x_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.27652
Sequence Type Exemplar sequence
Representative Public ID AV199932 NCBI
Target Description AV199932_rc /REP_DB=TREMBL Accession /GB=AV199932 /FEA=Transcript Cluster /DEF=Caenorhabditis elegans cDNA clone:yk553g4 : 3prime end, single read.
Cluster Evidence This cluster is supported by a Transcript Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Genome Target Overlap based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade B annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_065433
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
C16C10.3 ENSEMBL cdna:known chromosome:CEL140:III:4172305:4176130:-1 gene:C16C10.3 A 
NM_065433 NCBI Caenorhabditis elegans C16C10.3 (C16C10.3) mRNA, complete cds. None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:4172423-4172594(-) 52.09 52.09
Public Domain and Genome References
Gene Title
Ensembl C16C10.3
Entrez Gene 175535 Entrez gene
SwissProt Q09249 EMBL-EBI
WormBase CE01494 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA86741 Hypothetical protein C16C10.3 [Caenorhabditis elegans] ref|NP_497834.1| C16C10.3 [Caenorhabditis elegans] sp|Q09249|YQ53_CAEEL Hypothetical protein C16C10.3 in chromosome III 0.0
blast NP_497754 C14B1.7 [Caenorhabditis elegans] 0.0
blast CAE58274 Hypothetical protein CBG01380 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR003100 EMBL-EBI Argonaute and Dicer protein, PAZ 5.1E-54
Pfam IPR003165 EMBL-EBI Stem cell self-renewal protein Piwi 1.0E-126
Sequence
Target Sequence 
>C. ELEGANS:171903_X_AT
caccaccttaaactatctatacaagcacttctattcccccgcgtttggnnnncttgaaca
ttntttataaannnnnccccataattgtatcgtattnnnnnnnnnnnnnnnnnnnnnnnn
nntaagaatccttnttgtaatttaatctagagtagagtacattgacacatagtttgaata
gtagatactcctgttttacntgcnnnnnnnatgtgcgaatttcctccaactttcttatta
ggagtttccgatggcgtgtcgat

BLASTn GenBank NR
Note: "n"'s represent regions that are not probed by the probe sequences.
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CACCACCTTAAACTATCTATACAAG 349 201 21 Antisense
TAAACTATCTATACAAGCACTTCTA 247 639 29 Antisense
ATCTATACAAGCACTTCTATTCCCC 702 29 35 Antisense
TCTATACAAGCACTTCTATTCCCCC 445 615 36 Antisense
GAGTAGAGTACATTGACACATAGTT 46 399 170 Antisense
GAGTACATTGACACATAGTTTGAAT 551 399 175 Antisense
TTGACACATAGTTTGAATAGTAGAT 385 703 182 Antisense
TGAATAGTAGATACTCCTGTTTTAC 313 575 195 Antisense
TTTCCTCCAACTTTCTTATTAGGAG 379 669 240 Antisense
TATTAGGAGTTTCCGATGGCGTGTC 193 653 256 Antisense
TAGGAGTTTCCGATGGCGTGTCGAT 664 651 259 Antisense
Sequence Source Affymetrix Proprietary Database