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[171894_x_at]

Description CEK038E9R_rc /REP_DB=TREMBL Accession /GB=D33943 /FEA=Transcript Cluster /DEF=C.elegans cDNA clone yk38e9 : 3prime end, single read.
Cluster 6grp_highest_10_10 > 81      6grp_high1_10_10 > 29      4grp_highest_8_8 > 57      4grp_high_8_8 > 34      6grp_highest_nonorm > 80      6grp_total > 38      4grp_total > 46      6grp_highest_sub > Cu      6grp_cgc > 04      6grp_high1_sub > Cu      6grp_cgc_sub > Cu      6grp_total_sub > Cup      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 220
1 2 3 4 range
A 328.2
(3)
P 		240.5
(0.3)
P 		135.7
(-3)
P 		146.3
(-2.7)
P 		193
B 341.2
(3)
P 		270.9
(0.8)
P 		151.7
(-3)
P 		189.1
(-1.8)
P 		190
C 355.3
(3)
P 		192.6
(-2.7)
P 		217.3
(-1.9)
P 		185.5
(-3)
P 		170
avr 341.6
(3) 		234.7
(-0.7) 		168.2
(-3) 		173.6
(-2.8) 		173

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 331
1 2 3 4 5 6 range
A 266
(3)
P 		238.9
(2.1)
P 		90.9
(-3)
P 		102.6
(-2.6)
P 		230.2
(1.8)
P 		234.2
(1.9)
P 		175
B 331.6
(3)
P 		318.1
(2.7)
P 		77.5
(-3)
P 		133.3
(-1.7)
P 		145.5
(-1.4)
P 		213.9
(0.2)
P 		254
C 408.5
(3)
P 		252.1
(-0)
P 		108.6
(-2.8)
P 		97.7
(-3)
P 		232.5
(-0.4)
P 		203.2
(-1)
P 		311
avr 335.4
(3) 		269.7
(1.4) 		92.3
(-3) 		111.2
(-2.5) 		202.7
(-0.3) 		217.1
(0.1) 		243
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 98
1 2 3 4 range
A 119.5
(3)
P 		52.2
(-1.1)
P 		35.5
(-2.1)
P 		21.4
(-3)
P 		98
B 108.8
(3)
P 		66.9
(-0.4)
P 		35.1
(-3)
P 		40.3
(-2.6)
P 		74
C 117.6
(3)
P 		55.4
(-1.3)
P 		75.7
(0.1)
P 		31.3
(-3)
P 		86
avr 115.3
(3) 		58.2
(-1.1) 		48.8
(-1.7) 		31
(-3) 		84

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 133
1 2 3 4 5 6 range
A 49
(-0.3)
P 		85
(3)
P 		19.8
(-3)
P 		26
(-2.4)
P 		69.5
(1.6)
P 		57
(0.4)
P 		65
B 115.4
(3)
P 		105.9
(2.4)
P 		18.8
(-3)
P 		35.3
(-2)
P 		59.1
(-0.5)
P 		79.8
(0.8)
P 		97
C 151.4
(3)
P 		76.9
(-0.4)
P 		27.6
(-2.6)
P 		19.6
(-3)
P 		75.2
(-0.5)
P 		68
(-0.8)
P 		132
avr 105.3
(3) 		89.3
(1.8) 		22.1
(-3) 		27
(-2.6) 		67.9
(0.3) 		68.3
(0.3) 		83
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 171894_x_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.27925
Sequence Type Exemplar sequence
Representative Public ID CEK038E9R NCBI
Target Description CEK038E9R_rc /REP_DB=TREMBL Accession /GB=D33943 /FEA=Transcript Cluster /DEF=C.elegans cDNA clone yk38e9 : 3prime end, single read.
Cluster Evidence This cluster is supported by a Transcript Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_062322(10)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_062322 NCBI Caenorhabditis elegans Temporarily Assigned Gene name family member (tag-127) (tag-127) mRNA, complete cds. 10/11 A 
T08H4.1 ENSEMBL cdna:known chromosome:CEL140:II:4120331:4127843:1 gene:T08H4.1 10/11 A 
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000025211 1/11 Cross Hyb Matching Probes None
GENEFINDER00000025218 1/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:4125380-4127833(+) 99.2 99.47
Public Domain and Genome References
Gene Title
Gene Symbol tag-127
Ensembl T08H4.1
Entrez Gene 173748 Entrez gene
SwissProt Q22354 EMBL-EBI
WormBase CE04926 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAC71118 Temporarily assigned gene name protein 127 [Caenorhabditis elegans] ref|NP_494723.1| Temporarily Assigned Gene name family member (tag-127) [Caenorhabditis elegans] 0.0
blast CAE71513 Hypothetical protein CBG18446 [Caenorhabditis briggsae] 0.0
blast NP_001004156 neuronal RhoA GEF protein [Mus musculus] gb|AAU04954.1| RhoA-specific guanine exchange factor SYX2 [Mus musculus] 1.0E-51
Protein Domains
Database ID Description E-Value
Pfam IPR000219 EMBL-EBI DH domain 3.8E-7
Sequence
Target Sequence 
>C. ELEGANS:171894_X_AT
tattcaggataatcgtctagagacgtcagacgtctaactcccgccaatcatcacaatttt
cctcaaaacgatcagnnnnngtatatactagcaactgcccacttcnnnnnnnnntcgcca
tttcacattttctcatannnnnnnnnnnnnnnnnnnnnncgcacaaaagatatattcctg
cacacaggtagtattataaaatcggtaatacgcgcactgtttcttgtgatataaattgaa
tttcttcctaaatacgaacta

BLASTn GenBank NR
Note: "n"'s represent regions that are not probed by the probe sequences.
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TATTCAGGATAATCGTCTAGAGACG 494 659 42 Antisense
GAGACGTCAGACGTCTAACTCCCGC 518 391 61 Antisense
CGCCAATCATCACAATTTTCCTCAA 296 255 83 Antisense
TCACAATTTTCCTCAAAACGATCAG 124 629 92 Antisense
GTATATACTAGCAACTGCCCACTTC 360 453 122 Antisense
ACAAAAGATATATTCCTGCACACAG 129 111 204 Antisense
CCTGCACACAGGTAGTATTATAAAA 47 267 218 Antisense
AATCGGTAATACGCGCACTGTTTCT 433 169 241 Antisense
ATACGCGCACTGTTTCTTGTGATAT 575 23 249 Antisense
GCACTGTTTCTTGTGATATAAATTG 100 315 255 Antisense
TGAATTTCTTCCTAAATACGAACTA 554 573 278 Antisense
Sequence Source Affymetrix Proprietary Database