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[171892_x_at] Glutamine synthetase

Description CEK064D5R_rc /REP_DB=TREMBL Accession /GB=D71255 /FEA=Transcript Cluster /DEF=C.elegans cDNA clone yk64d5 : 3prime end, single read.
Cluster 6grp_highest_10_10 > 50      6grp_high1_10_10 > 16      4grp_highest_8_8 > 0      4grp_high_8_8 > 44      6grp_highest_nonorm > 50      6grp_total > 06      4grp_total > 44      6grp_highest_sub > De      6grp_total_sub > Decrease      6grp_cgc > 22      6grp_high1_sub > De      6grp_cgc_sub > De      6grp_rule_based > S1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 283
1 2 3 4 range
A 736.1
(-3)
P 		885.8
(3)
P 		771.5
(-1.6)
P 		738.1
(-2.9)
P 		150
B 756.1
(3)
P 		667.6
(-0.5)
P 		717.1
(1.5)
P 		603.1
(-3)
P 		153
C 736
(0.9)
P 		754.3
(2.2)
P 		676.1
(-3)
P 		767.1
(3)
P 		91
avr 742.7
(0.6) 		769.2
(3) 		721.6
(-1.3) 		702.8
(-3) 		66

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 656
1 2 3 4 5 6 range
A 985.1
(3)
P 		614.6
(-0.5)
P 		533.5
(-1.3)
P 		377.1
(-2.7)
P 		347.9
(-3)
P 		365.2
(-2.8)
P 		637
B 892.9
(3)
P 		764.5
(1.4)
P 		428.7
(-2.7)
P 		402.4
(-3)
P 		411.5
(-2.9)
P 		409.9
(-2.9)
P 		491
C 916.2
(3)
P 		684.6
(0.6)
P 		472.6
(-1.5)
P 		329.4
(-3)
P 		412.7
(-2.1)
P 		333.7
(-3)
P 		587
avr 931.4
(3) 		687.9
(0.4) 		478.3
(-1.8) 		369.6
(-3) 		390.7
(-2.8) 		369.6
(-3) 		562
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 160
1 2 3 4 range
A 268
(3)
P 		192.3
(0.2)
P 		201.7
(0.5)
P 		108
(-3)
P 		160
B 241.1
(3)
P 		164.9
(-1.1)
P 		166.2
(-1)
P 		128.5
(-3)
P 		113
C 243.6
(3)
P 		216.9
(1.6)
P 		235.7
(2.6)
P 		129.3
(-3)
P 		114
avr 250.9
(3) 		191.4
(0.2) 		201.2
(0.7) 		121.9
(-3) 		129

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 273
1 2 3 4 5 6 range
A 181.6
(1.3)
P 		218.6
(3)
P 		116.2
(-1.7)
P 		95.6
(-2.7)
P 		105
(-2.3)
P 		88.9
(-3)
P 		130
B 310.7
(3)
P 		254.5
(1.4)
P 		104.1
(-3)
P 		106.6
(-2.9)
P 		167.2
(-1.2)
P 		152.8
(-1.6)
P 		207
C 339.6
(3)
P 		208.9
(0.1)
P 		120.3
(-1.8)
P 		66.2
(-3)
P 		133.5
(-1.5)
P 		111.7
(-2)
P 		273
avr 277.3
(3) 		227.3
(1.4) 		113.5
(-2.2) 		89.5
(-3) 		135.2
(-1.5) 		117.8
(-2.1) 		188
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 171892_x_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.27826
Sequence Type Exemplar sequence
Representative Public ID CEK064D5R NCBI
Target Description CEK064D5R_rc /REP_DB=TREMBL Accession /GB=D71255 /FEA=Transcript Cluster /DEF=C.elegans cDNA clone yk64d5 : 3prime end, single read.
Cluster Evidence This cluster is supported by a Transcript Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_076611(10)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_076611 NCBI Caenorhabditis elegans GLutamiNe synthetase (glutamate-ammonia ligase) family member (gln-1) (gln-1) mRNA, complete cds. 10/11 A 
C45B2.5.1 ENSEMBL cdna:known chromosome:CEL140:X:6058212:6060189:1 gene:C45B2.5 10/11 A 
C45B2.5.2 ENSEMBL cdna:known chromosome:CEL140:X:6058212:6060136:1 gene:C45B2.5 9/11 A 
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
GENEFINDER00000012829 6/11 Cross Hyb Matching Probes None
SNAP00000012817 6/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:6059547-6060189(+) 100.0 100.0
Public Domain and Genome References
Gene Title Glutamine synthetase
Gene Symbol gln-1
Ensembl C45B2.5
Entrez Gene 180875 Entrez gene
SwissProt Q95YD1 EMBL-EBI
WormBase CE27854 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
9399 nitrogen fixation inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4356 glutamate-ammonia ligase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAK68249 Glutamine synthetase (glutamate-ammonia ligase) protein 1 [Caenorhabditis elegans] ref|NP_509012.1| GLutamiNe synthetase (glutamate-ammonia ligase) family member (gln-1) [Caenorhabditis elegans] 0.0
blast CAE68858 Hypothetical protein CBG14820 [Caenorhabditis briggsae] 0.0
blast CAB60321 Hypothetical protein Y105C5B.28 [Caenorhabditis elegans] ref|NP_502917.1| GLutamiNe synthetase (glutamate-ammonia ligase) family member (gln-3) [Caenorhabditis elegans] 1.0E-122
Protein Domains
Database ID Description E-Value
Pfam IPR008147 EMBL-EBI Glutamine synthetase, beta-Grasp domain 5.1E-25
Pfam IPR008146 EMBL-EBI Glutamine synthetase, catalytic domain 9.0E-74
Sequence
Target Sequence 
>C. ELEGANS:171892_X_AT
gtgaagccagtgttcgaattcctcgacaagtttttgctgataaatgtggctactttgaag
accgtcgtccggcgtccaactgtgatccgtacatggtaacgtcggcaatggtgaaaacgt
gctgtctggagggagaagacgcaaaactcactttacgctacgttccgaaattttaaatat
gcaattgtcagtctaattatatcgttgtgaaatgtgaaaagtgacaaagaatctcaattc
ctatatccttaaccccgtcttaccnttttgatggctttctctccaattagtaccatccga
t

BLASTn GenBank NR
Note: "n"'s represent regions that are not probed by the probe sequences.
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTGAAGCCAGTGTTCGAATTCCTCG 561 481 18 Antisense
GAATTCCTCGACAAGTTTTTGCTGA 7 325 33 Antisense
TACTTTGAAGACCGTCGTCCGGCGT 40 617 68 Antisense
CGTCCAACTGTGATCCGTACATGGT 22 245 90 Antisense
GTACATGGTAACGTCGGCAATGGTG 7 461 106 Antisense
GGTGAAAACGTGCTGTCTGGAGGGA 225 495 127 Antisense
AGACGCAAAACTCACTTTACGCTAC 457 53 154 Antisense
CTCACTTTACGCTACGTTCCGAAAT 621 227 164 Antisense
ATCTCAATTCCTATATCCTTAACCC 511 31 248 Antisense
TTTTGATGGCTTTCTCTCCAATTAG 573 677 283 Antisense
TTCTCTCCAATTAGTACCATCCGAT 146 691 294 Antisense
Sequence Source Affymetrix Proprietary Database