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[171881_x_at] Na(+)/K(+) ATPase alpha subunit

Description CEK053A5R_rc /REP_DB=TREMBL Accession /GB=D64530 /FEA=Transcript Cluster /DEF=C.elegans cDNA clone yk53a5 : 3prime end, single read.
Cluster 6grp_high1_10_10 > 03      4grp_highest_8_8 > 56      4grp_high_8_8 > 34      6grp_total > 25      4grp_total > 45      6grp_cgc > 24      6grp_high1_sub > De      6grp_cgc_sub > Cu      6grp_total_sub > Decrease      6grp_rule_based > S1      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 194
1 2 3 4 range
A 490.5
(3)
P 		488.3
(2.8)
P 		421.2
(-2.7)
P 		417.4
(-3)
P 		73
B 502.4
(3)
P 		450
(1)
P 		386.2
(-1.5)
P 		345.8
(-3)
P 		157
C 539.4
(3)
P 		482.6
(0.7)
P 		388.7
(-3)
P 		427.1
(-1.5)
P 		151
avr 510.8
(3) 		473.6
(1) 		398.7
(-2.9) 		396.8
(-3) 		114

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 466
1 2 3 4 5 6 range
A 587.9
(3)
P 		445
(0.8)
P 		312.8
(-1.3)
P 		329.8
(-1.1)
A 		206.4
(-3)
P 		351.8
(-0.7)
P 		382
B 439.4
(3)
P 		390.9
(1.8)
P 		353.4
(0.8)
P 		205.5
(-3)
P 		257.4
(-1.7)
P 		306.1
(-0.4)
P 		234
C 490.3
(3)
P 		422.8
(1.9)
P 		366.4
(1)
P 		406.6
(1.6)
P 		205.7
(-1.6)
P 		122.1
(-3)
P 		368
avr 505.9
(3) 		419.6
(1.2) 		344.2
(-0.4) 		314
(-1.1) 		223.2
(-3) 		260
(-2.2) 		283
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 118
1 2 3 4 range
A 178.6
(3)
P 		106
(-0.7)
P 		110.1
(-0.5)
P 		61.1
(-3)
P 		118
B 160.2
(3)
P 		111.1
(-0.4)
P 		89.5
(-1.9)
P 		73.7
(-3)
P 		87
C 178.6
(3)
P 		138.8
(0.8)
P 		135.5
(0.6)
P 		72
(-3)
P 		107
avr 172.5
(3) 		118.6
(-0.1) 		111.7
(-0.5) 		68.9
(-3) 		104

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 141
1 2 3 4 5 6 range
A 108.4
(-0.1)
P 		158.3
(3)
P 		68.1
(-2.6)
P 		83.6
(-1.7)
A 		62.3
(-3)
P 		85.7
(-1.5)
P 		96
B 152.9
(3)
P 		130.1
(1.6)
P 		85.8
(-1.1)
P 		54.4
(-3)
P 		104.6
(0.1)
P 		114.1
(0.6)
P 		99
C 181.7
(3)
P 		129
(0.8)
P 		93.2
(-0.8)
P 		81.8
(-1.3)
P 		66.6
(-1.9)
P 		40.9
(-3)
P 		141
avr 147.7
(3) 		139.1
(2.3) 		82.4
(-2.3) 		73.3
(-3) 		77.8
(-2.6) 		80.2
(-2.4) 		74
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 171881_x_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.28049
Sequence Type Exemplar sequence
Representative Public ID CEK053A5R NCBI
Target Description CEK053A5R_rc /REP_DB=TREMBL Accession /GB=D64530 /FEA=Transcript Cluster /DEF=C.elegans cDNA clone yk53a5 : 3prime end, single read.
Cluster Evidence This cluster is supported by a Transcript Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Genome Target Overlap based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade B annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_073868
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
B0365.3.2 ENSEMBL cdna:known chromosome:CEL140:V:13128997:13132587:-1 gene:B0365.3 A 
B0365.3.3 ENSEMBL cdna:known chromosome:CEL140:V:13129056:13132585:-1 gene:B0365.3 None
NM_073868 NCBI Caenorhabditis elegans EATing: abnormal pharyngeal pumping family member (eat-6) (eat-6) mRNA, complete cds. A 
SNAP00000032430 ENSEMBL cdna:SNAP chromosome:CEL140:V:13129236:13132577:-1 None
GENEFINDER00000032438 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:13129236:13132577:-1 None
B0365.3.1 ENSEMBL cdna:known chromosome:CEL140:V:13128997:13132585:-1 gene:B0365.3 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:13129066-13129360(-) 87.04 87.04
Public Domain and Genome References
Gene Title Na(+)/K(+) ATPase alpha subunit
Gene Symbol eat-6
Ensembl B0365.3
Entrez Gene 179796 Entrez gene
SwissProt P90735 EMBL-EBI
WormBase CE07721 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE_2:CFAAFFX.8375.1.S1_AT similar to Na+/K+ -ATPase alpha 3 subunit cfa
DROSOPHILA_2:1625719_AT Na pump subunit dm
DROSOPHILA_2:1639145_S_AT Na pump subunit dm
DROSGENOME1:142520_AT Na pump subunit dm
HUGENEFL:M37457_AT ATPase, Na+/K+ transporting, alpha 3 polypeptide hs
HUGENEFL:M37457_S_AT ATPase, Na+/K+ transporting, alpha 3 polypeptide hs
U133_X3P:HS.274371.0.S1_3P_X_AT ATPase, Na+/K+ transporting, alpha 3 polypeptide hs
U133_X3P:HS.274371.0.S1_3P_AT ATPase, Na+/K+ transporting, alpha 3 polypeptide hs
U133_X3P:214432_3P_AT ATPase, Na+/K+ transporting, alpha 3 polypeptide hs
HG-U133A_2:214432_AT ATPase, Na+/K+ transporting, alpha 3 polypeptide hs
HG-FOCUS:214432_AT ATPase, Na+/K+ transporting, alpha 3 polypeptide hs
HG-U133_PLUS_2:214432_AT ATPase, Na+/K+ transporting, alpha 3 polypeptide hs
HG-U133A:214432_AT ATPase, Na+/K+ transporting, alpha 3 polypeptide hs
HG-U95AV2:35670_AT ATPase, Na+/K+ transporting, alpha 3 polypeptide hs
MOUSE430_2:1427481_A_AT ATPase, Na+/K+ transporting, alpha 3 polypeptide mm
MOUSE430A_2:1427481_A_AT ATPase, Na+/K+ transporting, alpha 3 polypeptide mm
MOE430A:1427481_A_AT ATPase, Na+/K+ transporting, alpha 3 polypeptide mm
MU11KSUBB:MSA.11233.0_AT ATPase, Na+/K+ transporting, alpha 3 polypeptide mm
MG-U74BV2:106975_AT ATPase, Na+/K+ transporting, alpha 3 polypeptide mm
MOE430A:1424856_AT ATPase, Na+/K+ transporting, alpha 3 polypeptide mm
MOUSE430A_2:1424856_AT ATPase, Na+/K+ transporting, alpha 3 polypeptide mm
MOUSE430_2:1424856_AT ATPase, Na+/K+ transporting, alpha 3 polypeptide mm
RN-U34:M28648_S_AT ATPase, Na+/K+ transporting, alpha 3 polypeptide rn
RT-U34:M28648_S_AT ATPase, Na+/K+ transporting, alpha 3 polypeptide rn
RG-U34A:M28648_S_AT ATPase, Na+/K+ transporting, alpha 3 polypeptide rn
RG-U34A:D00189_AT ATPase, Na+/K+ transporting, alpha 3 polypeptide rn
RAE230A:1368701_AT ATPase, Na+/K+ transporting, alpha 3 polypeptide rn
RAT230_2:1368701_AT ATPase, Na+/K+ transporting, alpha 3 polypeptide rn
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6812 cation transport inferred from electronic annotation QuickGO AmiGO
8152 metabolism inferred from electronic annotation QuickGO AmiGO
15672 monovalent inorganic cation transport inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
16020 membrane inferred from electronic annotation QuickGO AmiGO
16021 integral to membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
3824 catalytic activity inferred from electronic annotation QuickGO AmiGO
5524 ATP binding inferred from electronic annotation QuickGO AmiGO
15077 monovalent inorganic cation transporter activity inferred from electronic annotation QuickGO AmiGO
15662 ATPase activity, coupled to transmembrane movement of ions, phosphorylative mechanism inferred from electronic annotation QuickGO AmiGO
16820 hydrolase activity, acting on acid anhydrides, catalyzing transmembrane movement of substances inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB02694 Hypothetical protein B0365.3 [Caenorhabditis elegans] ref|NP_506269.1| EATing: abnormal pharyngeal pumping family member (eat-6) [Caenorhabditis elegans] 0.0
blast AAB02615 Na,K-ATPase alpha subunit 0.0
blast CAE64662 Hypothetical protein CBG09434 [Caenorhabditis briggsae] 0.0
Protein Families
Method ID Description E-Value
ec A1A1_HUMAN (P05023) Sodium/potassium-transporting ATPase alpha-1 chain precursor (EC 3.6.3.9) (Sodium pump 1) (Na+/K+ ATPase 1) 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR006068 EMBL-EBI Cation transporting ATPase, C-terminal 4.7E-74
Pfam IPR008250 EMBL-EBI E1-E2 ATPase-associated region 1.4E-117
Pfam IPR005834 EMBL-EBI Haloacid dehalogenase-like hydrolase 8.3E-9
Pfam IPR005834 EMBL-EBI Haloacid dehalogenase-like hydrolase 2.0E-11
Pfam IPR004014 EMBL-EBI Cation transporting ATPase, N-terminal 3.8E-32
Pfam IPR006068 EMBL-EBI Cation transporting ATPase, C-terminal 3.6E-69
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_506269.1 8 73-95,108-127,269-291,301-323,823-845,886-905,925-947,957-974
B0365.3.1 8 73-95,108-127,269-291,301-323,823-845,886-905,925-947,957-974
B0365.3.2 8 73-95,108-127,269-291,301-323,823-845,886-905,925-947,957-974
B0365.3.3 8 73-95,108-127,269-291,301-323,823-845,886-905,925-947,957-974
SNAP00000032430 8 73-95,108-127,269-291,301-323,823-845,891-910,930-952,962-979
GENEFINDER00000032438 8 73-95,108-127,269-291,301-323,823-845,886-905,925-947,957-974
Sequence
Target Sequence 
>C. ELEGANS:171881_X_AT
gaactcagattctcctggtggttctgtgcccttccattctcaatcttcatcttcgcctac
gacgagatccgtcgtttcttgattcgcagatatccaggaggatgggtcgaccgtgagacc
tcctactaaagaacagcttgtgaatctttgtagaattttctannnnnnatctnagttatt
tattgtttccaatctcatcccgatctaataatttctttcatttagtgatcctactttctg
tcgtcgttttgtgaactttgtattccattcccgaaaatccaagccga

BLASTn GenBank NR
Note: "n"'s represent regions that are not probed by the probe sequences.
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAACTCAGATTCTCCTGGTGGTTCT 391 349 20 Antisense
TCTTCGCCTACGACGAGATCCGTCG 205 617 69 Antisense
ATCCGTCGTTTCTTGATTCGCAGAT 417 39 86 Antisense
ATCCAGGAGGATGGGTCGACCGTGA 660 37 111 Antisense
GACCGTGAGACCTCCTACTAAAGAA 531 377 128 Antisense
TTTATTGTTTCCAATCTCATCCCGA 332 675 198 Antisense
TTTCCAATCTCATCCCGATCTAATA 482 669 205 Antisense
TTTAGTGATCCTACTTTCTGTCGTC 185 673 240 Antisense
TTCTGTCGTCGTTTTGTGAACTTTG 67 695 255 Antisense
GTGAACTTTGTATTCCATTCCCGAA 222 481 270 Antisense
TTCCATTCCCGAAAATCCAAGCCGA 105 695 282 Antisense
Sequence Source Affymetrix Proprietary Database