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[171874_x_at]

Description CEK002GZR_rc /REP_DB=TREMBL Accession /GB=D33372 /FEA=Transcript Cluster /DEF=C.elegans cDNA clone yk2g12 : 3prime end, single read.
Cluster 6grp_high1_10_10 > 14      4grp_highest_8_8 > 40      4grp_high_8_8 > 43      6grp_total > 02      4grp_total > 52      6grp_cgc > 51      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 176
1 2 3 4 range
A 150.2
(1.9)
P 		142.3
(1.2)
P 		161.7
(3)
P 		97.9
(-3)
P 		64
B 274.1
(3)
P 		250.3
(2.2)
P 		146.5
(-1.4)
P 		100.5
(-3)
P 		174
C 141.9
(-3)
P 		187.1
(3)
P 		141.9
(-3)
P 		152.4
(-1.6)
P 		45
avr 188.7
(2.6) 		193.2
(3) 		150
(-0.4) 		116.9
(-3) 		76

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 126
1 2 3 4 5 6 range
A 178.8
(3)
P 		88.5
(-2)
P 		94.6
(-1.7)
P 		130.7
(0.3)
P 		166.6
(2.3)
P 		70.9
(-3)
P 		108
B 140.5
(0.4)
P 		193.8
(3)
P 		81.1
(-2.5)
P 		70.2
(-3)
P 		123.6
(-0.4)
P 		69.8
(-3)
P 		124
C 88.6
(-1.5)
P 		139.8
(2.3)
P 		107.3
(-0.1)
P 		149.4
(3)
A 		77.7
(-2.3)
P 		68
(-3)
M 		81
avr 136
(2.6) 		140.7
(3) 		94.3
(-0.9) 		116.8
(1) 		122.6
(1.5) 		69.6
(-3) 		71
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 73
1 2 3 4 range
A 54.7
(3)
P 		30.9
(-0.5)
P 		42.3
(1.2)
P 		14.3
(-3)
P 		40
B 87.4
(3)
P 		61.8
(0.7)
P 		34
(-1.9)
P 		21.4
(-3)
P 		66
C 47
(1.5)
P 		53.8
(3)
P 		49.4
(2.1)
P 		25.7
(-3)
P 		28
avr 63
(3) 		48.8
(1) 		41.9
(0) 		20.5
(-3) 		43

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 47
1 2 3 4 5 6 range
A 33
(-0.1)
P 		31.5
(-0.4)
P 		20.6
(-2.4)
P 		33.2
(-0.1)
P 		50.3
(3)
P 		17.3
(-3)
P 		33
B 48.9
(1)
P 		64.5
(3)
P 		19.7
(-2.9)
P 		18.6
(-3)
P 		50.2
(1.1)
P 		26
(-2)
P 		46
C 32.9
(0.1)
P 		42.6
(3)
P 		27.3
(-1.6)
P 		30
(-0.8)
A 		25.2
(-2.3)
P 		22.8
(-3)
M 		20
avr 38.3
(1) 		46.2
(3) 		22.5
(-2.9) 		27.3
(-1.7) 		41.9
(1.9) 		22
(-3) 		24
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 171874_x_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.28061
Sequence Type Exemplar sequence
Representative Public ID CEK002GZR NCBI
Target Description CEK002GZR_rc /REP_DB=TREMBL Accession /GB=D33372 /FEA=Transcript Cluster /DEF=C.elegans cDNA clone yk2g12 : 3prime end, single read.
Cluster Evidence This cluster is supported by a Transcript Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Genome Target Overlap based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade B annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_182443, NM_076922
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
R03G5.1c.2 ENSEMBL cdna:known chromosome:CEL140:X:7823593:7825708:1 gene:R03G5.1 None
R03G5.1a.1 ENSEMBL cdna:known chromosome:CEL140:X:7823593:7825699:1 gene:R03G5.1 None
NM_076922 NCBI Caenorhabditis elegans Elongation FacTor family member (eft-4) (eft-4) mRNA, complete cds. None
NM_182443 NCBI Caenorhabditis elegans Elongation FacTor family member (eft-4) (eft-4) mRNA, complete cds. None
R03G5.1a.3 ENSEMBL cdna:known chromosome:CEL140:X:7823587:7825688:1 gene:R03G5.1 None
R03G5.1a.2 ENSEMBL cdna:known chromosome:CEL140:X:7823588:7825688:1 gene:R03G5.1 None
R03G5.1c.1 ENSEMBL cdna:known chromosome:CEL140:X:7823589:7825708:1 gene:R03G5.1 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:7825338-7825688(+) 89.17 89.17
Public Domain and Genome References
Ensembl R03G5.1
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAA81688 Elongation factor protein 3 [Caenorhabditis elegans] gb|AAA96068.1| Elongation factor protein 4, isoform a [Caenorhabditis elegans] ref|NP_509323.1| Elongation FacTor family member (eft-4) [Caenorhabditis elegans] ref|NP_498520.1| Elongation FacTor family member (eft-3) [Caenorhabditis elegans] sp|P53013|EF1A_CAEEL Elongation factor 1-alpha (EF-1-alpha) 0.0
blast CAE70307 Hypothetical protein CBG16828 [Caenorhabditis briggsae] emb|CAE70057.1| Hypothetical protein CBG16491 [Caenorhabditis briggsae] emb|CAE56763.1| Hypothetical protein CBG24566 [Caenorhabditis briggsae] 0.0
blast P27592 Elongation factor 1-alpha (EF-1-alpha) gb|AAA29416.1| elongation factor 0.0
Protein Domains
Database ID Description E-Value
scop a.4.5.Transcription factor MotA, activation domain All alpha proteins; DNA/RNA-binding 3-helical bundle; "Winged helix" DNA-binding domain; Transcription factor MotA, activation domain 1.20000004768372
Pfam IPR004161 EMBL-EBI Elongation factor Tu, domain 2 1.3E-25
Pfam IPR000795 EMBL-EBI Protein synthesis factor, GTP-binding 2.1E-112
Pfam IPR004160 EMBL-EBI Elongation factor Tu, C-terminal 6.4E-57
Pfam IPR000795 EMBL-EBI Protein synthesis factor, GTP-binding 3.5E-81
Sequence
Target Sequence 
>C. ELEGANS:171874_X_AT
ggtcattgtttattttgtccaagcagccttttgaacaaaagttgtgngtttgggcaaaaa
nnnnnngtttccnnnnnccgtccatccccnnnnncctttggaaagtatttattaagggtt
gttatgttttccccaaaacaaaggggcttnnatccaaaataataaanacagttaattgta
ccatttgattatcaataannnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnn
ntcctttnnnnnctgttatgttaatatgtcatgcattgttttgtcgcattctgtcatcca
tcgttttgtgggcgtaagtttgt

BLASTn GenBank NR
Note: "n"'s represent regions that are not probed by the probe sequences.
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GGTCATTGTTTATTTTGTCCAAGCA 531 503 20 Antisense
GTTTATTTTGTCCAAGCAGCCTTTT 593 449 27 Antisense
TATTTTGTCCAAGCAGCCTTTTGAA 376 661 30 Antisense
TTTTGTCCAAGCAGCCTTTTGAACA 598 673 32 Antisense
TGTCCAAGCAGCCTTTTGAACAAAA 129 557 35 Antisense
GTTAATTGTACCATTTGATTATCAA 512 445 190 Antisense
AATATGTCATGCATTGTTTTGTCGC 380 173 282 Antisense
CATTGTTTTGTCGCATTCTGTCATC 632 217 293 Antisense
CATTCTGTCATCCATCGTTTTGTGG 369 217 306 Antisense
CATCCATCGTTTTGTGGGCGTAAGT 542 209 314 Antisense
CATCGTTTTGTGGGCGTAAGTTTGT 680 45 318 Antisense
Sequence Source Affymetrix Proprietary Database