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[171856_x_at]

Description CEC1293_rc /REP_DB=TREMBL Accession /GB=C11129 /FEA=Transcript Cluster /DEF=C.elegans cDNA clone yk164d4 : 3prime end, single read.
Cluster 6grp_highest_10_10 > 03      6grp_high1_10_10 > 31      4grp_highest_8_8 > 29      4grp_high_8_8 > 07      6grp_highest_nonorm > 05      6grp_total > 32      4grp_total > 06      6grp_highest_sub > De      6grp_cgc > 80      6grp_high1_sub > 03      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 3100
1 2 3 4 range
A 6852.4
(-2.3)
P 		6600.4
(-3)
P 		6613.8
(-3)
P 		8702.9
(3)
P 		2103
B 7302.1
(1.5)
P 		5603.1
(-3)
P 		6824.3
(0.3)
P 		7855.6
(3)
P 		2253
C 6323.6
(-0.5)
P 		5840
(-3)
P 		6540.9
(0.6)
P 		7015.2
(3)
P 		1175
avr 6826
(-0.4) 		6014.5
(-3) 		6659.7
(-0.9) 		7857.9
(3) 		1843

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 5727
1 2 3 4 5 6 range
A 6298.2
(-0.5)
P 		7359.3
(0.6)
P 		9640.6
(3)
P 		5929.9
(-0.9)
P 		5187.7
(-1.7)
P 		3914.1
(-3)
P 		5727
B 7154.9
(0.5)
P 		6785.7
(0)
P 		9040.6
(3)
P 		6015.2
(-1)
P 		6783.3
(0)
P 		4475
(-3)
P 		4566
C 9070.6
(3)
P 		6552.1
(-0.4)
P 		6521.7
(-0.5)
P 		5872.9
(-1.4)
P 		8490.3
(2.2)
P 		4678.8
(-3)
P 		4392
avr 7507.9
(1.7) 		6899
(0.8) 		8401
(3) 		5939.3
(-0.7) 		6820.4
(0.7) 		4356
(-3) 		4045
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1312
1 2 3 4 range
A 2495
(3)
P 		1432.8
(-2.2)
P 		1729
(-0.8)
P 		1273.9
(-3)
P 		1221
B 2328.6
(3)
P 		1383.8
(-3)
P 		1581.4
(-1.7)
P 		1674
(-1.2)
P 		945
C 2093.3
(2)
P 		1679
(-0.3)
P 		2279.7
(3)
P 		1182.9
(-3)
P 		1097
avr 2305.6
(3) 		1498.5
(-2.2) 		1863.4
(0.1) 		1376.9
(-3) 		929

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 2409
1 2 3 4 5 6 range
A 1161
(-2.3)
P 		2617.3
(3)
P 		2099.5
(1.1)
P 		1504
(-1)
P 		1565.4
(-0.8)
P 		953
(-3)
P 		1664
B 2489.6
(1.6)
P 		2258.7
(0.4)
P 		2195.3
(0.1)
P 		1593.2
(-3)
P 		2756
(3)
P 		1668.7
(-2.6)
P 		1163
C 3362.1
(3)
P 		1999.3
(-0.7)
P 		1659.9
(-1.7)
P 		1181
(-3)
P 		2747.3
(1.3)
P 		1566.3
(-1.9)
P 		2181
avr 2337.6
(2.9) 		2291.8
(2.6) 		1984.9
(0.7) 		1426.1
(-2.8) 		2356.2
(3) 		1396
(-3) 		960
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 171856_x_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.27757
Sequence Type Exemplar sequence
Representative Public ID CEC1293 NCBI
Target Description CEC1293_rc /REP_DB=TREMBL Accession /GB=C11129 /FEA=Transcript Cluster /DEF=C.elegans cDNA clone yk164d4 : 3prime end, single read.
Cluster Evidence This cluster is supported by a Transcript Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Genome Target Overlap based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade B annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_078428
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
GENEFINDER00000036960 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:17455812:17456478:-1 None
SNAP00000036943 ENSEMBL cdna:SNAP chromosome:CEL140:X:17459385:17460067:1 None
C36E6.3 ENSEMBL cdna:known chromosome:CEL140:X:17459085:17460757:1 gene:C36E6.3 A 
NM_078428 NCBI Caenorhabditis elegans Myosin Light Chain family member (mlc-1) (mlc-1) mRNA, complete cds. A 
GENEFINDER00000036964 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:17459385:17460067:1 None
SNAP00000036942 ENSEMBL cdna:SNAP chromosome:CEL140:X:17455812:17456478:-1 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_078427 6/11 Cross Hyb Matching Probes A 
C36E6.5.3 6/11 Cross Hyb Matching Probes None
C36E6.5.2 6/11 Cross Hyb Matching Probes A 
C36E6.5.1 6/11 Cross Hyb Matching Probes A 
C36E6.5.4 6/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:17459780-17460092(+) 61.97 61.97
Public Domain and Genome References
Gene Title
Gene Symbol mlc-1
Ensembl C36E6.3
Entrez Gene 181776 Entrez gene
WormBase CE34269 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE_2:CFAAFFX.21284.1.S1_AT similar to myosin light chain 2, precursor lymphocyte-specific cfa
CHICKEN:GGA.13194.1.S1_AT similar to myosin L2B regulatory light chain, cardiac muscle - chicken gga
HG-U133_PLUS_2:221659_S_AT myosin light chain 2, precursor lymphocyte-specific hs
HG-U133A:221659_S_AT myosin light chain 2, precursor lymphocyte-specific hs
HG-U133A_2:221659_S_AT myosin light chain 2, precursor lymphocyte-specific hs
U133_X3P:G12803868_3P_A_AT myosin light chain 2, precursor lymphocyte-specific hs
HG-U133_PLUS_2:221660_AT myosin light chain 2, precursor lymphocyte-specific hs
HG-U133A:221660_AT myosin light chain 2, precursor lymphocyte-specific hs
HG-U133A_2:221660_AT myosin light chain 2, precursor lymphocyte-specific hs
MOE430A:1420805_AT myosin light chain 2, precursor lymphocyte-specific mm
MOUSE430_2:1420805_AT myosin light chain 2, precursor lymphocyte-specific mm
MOUSE430A_2:1420805_AT myosin light chain 2, precursor lymphocyte-specific mm
MU11KSUBB:X65979_S_AT myosin light chain 2, precursor lymphocyte-specific mm
MG-U74AV2:101195_AT myosin light chain 2, precursor lymphocyte-specific mm
Gene Ontology
GO Cellular Component (view graph)
ID Description Evidence Links
5859 muscle myosin inferred from direct assay QuickGO AmiGO
5859 muscle myosin inferred from sequence similarity QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
5509 calcium ion binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAK09069 Myosin light chain protein 2 [Caenorhabditis elegans] ref|NP_510828.1| Myosin Light Chain family member (mlc-2) [Caenorhabditis elegans] sp|P19626|MLR2_CAEEL Myosin regulatory light chain 2 gb|AAA28114.1| myosin light chain 2.0E-81
blast AAK09070 Myosin light chain protein 1 [Caenorhabditis elegans] ref|NP_510829.2| Myosin Light Chain family member (mlc-1) [Caenorhabditis elegans] sp|P19625|MLR1_CAEEL Myosin regulatory light chain 1 gb|AAA28113.1| myosin light chain 2.0E-81
Protein Domains
Database ID Description E-Value
Pfam IPR002048 EMBL-EBI Calcium-binding EF-hand 1.7E-6
Pfam IPR002048 EMBL-EBI Calcium-binding EF-hand 1.2
Pfam IPR002048 EMBL-EBI Calcium-binding EF-hand 1.3
Pfam IPR002048 EMBL-EBI Calcium-binding EF-hand 1.7E-6
Pfam IPR002048 EMBL-EBI Calcium-binding EF-hand 1.2
Sequence
Target Sequence 
>C. ELEGANS:171856_X_AT
gttggagcttttgctatgttcgataagaaggactgcggtaagatcaaggaggncgacctt
atcnagatccttccccncaagagaggagagccattagacgaggatgaggtcaaggctatg
tacaagggaaaagccaccaatcgagggaggagaggttgtctacaaggcattcgctcacct
catcaccaccggagcccaagacgagctacgccagcgcctaaacatccggcaattgcacac
aaatcttacattctttncaaataatacttcctcatttatttctccaattata

BLASTn GenBank NR
Note: "n"'s represent regions that are not probed by the probe sequences.
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTTGGAGCTTTTGCTATGTTCGATA 332 435 50 Antisense
GAAGGACTGCGGTAAGATCAAGGAG 72 335 76 Antisense
GAGAGGAGAGCCATTAGACGAGGAT 358 395 130 Antisense
GGATGAGGTCAAGGCTATGTACAAG 504 513 151 Antisense
GGGAAAAGCCACCAATCGAGGGAGG 59 487 175 Antisense
GTTGTCTACAAGGCATTCGCTCACC 551 435 204 Antisense
CCAGCGCCTAAACATCCGGCAATTG 632 271 260 Antisense
AACATCCGGCAATTGCACACAAATC 475 137 270 Antisense
ATTGCACACAAATCTTACATTCTTT 191 7 281 Antisense
AATACTTCCTCATTTATTTCTCCAA 65 173 312 Antisense
TTCCTCATTTATTTCTCCAATTATA 65 701 317 Antisense
Sequence Source Affymetrix Proprietary Database