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[171826_x_at]

Description CEC295_rc /REP_DB=TREMBL Accession /GB=C07295 /FEA=Transcript Cluster /DEF=C.elegans cDNA clone yk136d1 : 3prime end, single read.
Cluster 6grp_highest_10_10 > 97      6grp_high1_10_10 > 78      4grp_highest_8_8 > 02      4grp_high_8_8 > 46      6grp_highest_nonorm > 84      6grp_total > 59      4grp_total > 49      6grp_highest_sub > Cu      6grp_cgc > 39      6grp_high1_sub > Cu      6grp_cgc_sub > Cu      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 526
1 2 3 4 range
A 379.1
(-2.9)
P 		626.2
(1)
P 		758.2
(3)
P 		370.5
(-3)
P 		388
B 232
(-3)
P 		286.7
(-1.5)
P 		453.7
(3)
P 		451
(2.9)
P 		222
C 376.4
(-1.8)
P 		706.6
(3)
P 		304.9
(-2.9)
P 		295.5
(-3)
P 		411
avr 329.2
(-3) 		539.8
(3) 		505.6
(2) 		372.3
(-1.8) 		211

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 633
1 2 3 4 5 6 range
A 873.7
(2.8)
P 		272.3
(-2.9)
P 		389.6
(-1.7)
P 		257
(-3)
P 		469.5
(-1)
P 		890.3
(3)
P 		633
B 572.5
(-0.4)
P 		426.1
(-2)
P 		337.8
(-3)
P 		547.1
(-0.7)
P 		606.8
(-0)
P 		881.3
(3)
P 		544
C 587.9
(0.5)
P 		525.1
(-0.4)
P 		335.7
(-3)
P 		644.7
(1.3)
P 		595.4
(0.6)
P 		765.7
(3)
P 		430
avr 678
(1) 		407.8
(-2.3) 		354.4
(-3) 		482.9
(-1.4) 		557.2
(-0.5) 		845.8
(3) 		491
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 153
1 2 3 4 range
A 138
(0.5)
P 		135.9
(0.4)
P 		198.2
(3)
P 		54.2
(-3)
P 		144
B 74
(-2.4)
P 		70.8
(-3)
P 		105.1
(3)
P 		96.1
(1.4)
P 		34
C 124.6
(-0.1)
P 		203.1
(3)
P 		106.3
(-0.8)
P 		49.8
(-3)
P 		153
avr 112.2
(0.9) 		136.6
(3) 		136.5
(3) 		66.7
(-3) 		70

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 263
1 2 3 4 5 6 range
A 161.1
(0.8)
P 		96.9
(-1.7)
P 		84.8
(-2.2)
P 		65.2
(-3)
P 		141.7
(0)
P 		216.8
(3)
P 		152
B 199.2
(-0.1)
P 		141.8
(-1.5)
P 		82
(-3)
P 		144.9
(-1.5)
P 		246.5
(1)
P 		328.6
(3)
P 		247
C 217.9
(1.7)
P 		160.2
(-0.4)
P 		85.5
(-3)
P 		129.6
(-1.5)
P 		192.7
(0.8)
P 		256.3
(3)
P 		171
avr 192.7
(0.6) 		133
(-1.4) 		84.1
(-3) 		113.2
(-2) 		193.6
(0.6) 		267.2
(3) 		183
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 171826_x_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.28053
Sequence Type Exemplar sequence
Representative Public ID CEC295 NCBI
Target Description CEC295_rc /REP_DB=TREMBL Accession /GB=C07295 /FEA=Transcript Cluster /DEF=C.elegans cDNA clone yk136d1 : 3prime end, single read.
Cluster Evidence This cluster is supported by a Transcript Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Genome Target Overlap based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade B annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_071024
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
SNAP00000040088 ENSEMBL cdna:SNAP chromosome:CEL140:V:1476278:1479200:1 None
NM_071024 NCBI Caenorhabditis elegans UNCoordinated family member (unc-60) (unc-60) mRNA, complete cds. None
C38C3.5a ENSEMBL cdna:known chromosome:CEL140:V:1474915:1476608:1 gene:C38C3.5 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:1476251-1476556(+) 58.54 58.54
Public Domain and Genome References
Ensembl C38C3.5
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAL02461 Uncoordinated protein 60, isoform a [Caenorhabditis elegans] ref|NP_503425.2| UNCoordinated family member (unc-60) [Caenorhabditis elegans] sp|Q07750|ADF1_CAEEL Actin-depolymerizing factor 1 (Uncoordinated protein 60) gb|AAC14458.1| putative. cofilin/destrin homolog. This CDS encodes the first transcript produced from the unc-60 locus. Both transcripts exhibit cofilin/destrin homologies, and share only the 5'-most exon which encodes the initiator methionine [Caenorhabditis elegans] 5.0E-89
blast AAL02462 Uncoordinated protein 60, isoform b [Caenorhabditis elegans] ref|NP_503426.2| UNCoordinated family member (unc-60) [Caenorhabditis elegans] 5.0E-83
blast CAE62475 Hypothetical protein CBG06572 [Caenorhabditis briggsae] 1.0E-81
blast T33952 actin depolymerizing factor homolog unc-60 - Caenorhabditis elegans 2.0E-81
blast AAL02463 Uncoordinated protein 60, isoform c [Caenorhabditis elegans] ref|NP_503427.2| UNCoordinated family member (unc-60) [Caenorhabditis elegans] sp|Q07749|ADF2_CAEEL Actin-depolymerizing factor 2 (Uncoordinated protein 60) gb|AAC14457.1| This CDS encodes the second transcript produced from the unc-60 locus. Both transcripts exhibit cofilin/destrin homologies, and share only the 5'-most exon which encodes the initiator methionine. putative [Caenorhabditis elegans] 4.0E-80
blast CAE62476 Hypothetical protein CBG06573 [Caenorhabditis briggsae] 4.0E-78
Protein Domains
Database ID Description E-Value
Pfam IPR002108 EMBL-EBI Actin-binding, cofilin/tropomyosin type 1.2E-61
Pfam IPR002108 EMBL-EBI Actin-binding, cofilin/tropomyosin type 8.8E-65
Sequence
Target Sequence 
>C. ELEGANS:171826_X_AT
accttccgtacctatgaagaagttgagacatcccctcagattctcagtatctttattgtt
tagtgaaatgcgtggtaaatcccttcctcatttacgattatattactctccctcttgtaa
agcttt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ACCTTCCGTACCTATGAAGAAGTTG 140 83 50 Antisense
GAAGAAGTTGAGACATCCCCTCAGA 126 341 65 Antisense
TGAGACATCCCCTCAGATTCTCAGT 622 567 73 Antisense
ATCCCCTCAGATTCTCAGTATCTTT 328 39 79 Antisense
ATTCTCAGTATCTTTATTGTTTAGT 370 11 89 Antisense
GAAATGCGTGGTAAATCCCTTCCTC 637 361 114 Antisense
AAATCCCTTCCTCATTTACGATTAT 576 89 126 Antisense
TTACGATTATATTACTCTCCCTCTT 688 681 141 Antisense
ATTATATTACTCTCCCTCTTGTAAA 50 15 146 Antisense
ATATTACTCTCCCTCTTGTAAAGCT 19 19 149 Antisense
ATTACTCTCCCTCTTGTAAAGCTTT 604 11 151 Antisense
Sequence Source Affymetrix Proprietary Database