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[171802_s_at] Phosphoinositide 3-kinase family, accessory domain (PIK domain) ; Phosphatidylinositol 3- and 4-kinases

Description C33749_rc /REP_DB=TREMBL Accession /GB=C33749 /FEA=Transcript Cluster /DEF=C.elegans cDNA clone yk374b1 : 3prime end, single read.
Cluster 6grp_highest_10_10 > 61      6grp_high1_10_10 > 26      4grp_highest_8_8 > 41      4grp_high_8_8 > 49      6grp_highest_nonorm > 60      6grp_total > 26      4grp_total > 62      6grp_highest_sub > De      6grp_high1_sub > De      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 513
1 2 3 4 range
A 679.8
(3)
P 		297.9
(-1.5)
P 		393.5
(-0.3)
P 		166.5
(-3)
P 		513
B 425.4
(3)
P 		401.6
(2.1)
P 		390
(1.6)
P 		268.2
(-3)
P 		157
C 578.9
(3)
P 		478.9
(1.4)
P 		456.9
(1.1)
P 		195.3
(-3)
P 		384
avr 561.4
(3) 		392.8
(0.1) 		413.5
(0.5) 		210
(-3) 		351

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 449
1 2 3 4 5 6 range
A 405.9
(0.2)
P 		520.5
(3)
P 		305.6
(-2.3)
P 		274.9
(-3)
P 		356.9
(-1)
P 		374.7
(-0.6)
P 		246
B 590.8
(3)
P 		525.3
(1.5)
P 		432.4
(-0.5)
P 		380.8
(-1.7)
P 		322.3
(-3)
P 		369.9
(-1.9)
P 		269
C 723.7
(3)
P 		485.8
(-0.5)
P 		351.5
(-2.4)
P 		312.4
(-3)
P 		378.8
(-2)
P 		382
(-2)
P 		411
avr 573.5
(3) 		510.5
(1.5) 		363.2
(-2) 		322.7
(-3) 		352.7
(-2.3) 		375.5
(-1.7) 		251
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 223
1 2 3 4 range
A 247.5
(3)
P 		64.7
(-1.9)
P 		102.9
(-0.9)
P 		24.4
(-3)
P 		223
B 135.6
(3)
P 		99.2
(0.2)
P 		90.4
(-0.5)
P 		57.1
(-3)
P 		79
C 191.6
(3)
P 		137.7
(1)
P 		159.2
(1.8)
P 		32.9
(-3)
P 		159
avr 191.6
(3) 		100.5
(-0.6) 		117.5
(0.1) 		38.1
(-3) 		154

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 206
1 2 3 4 5 6 range
A 74.8
(-2.6)
P 		185.1
(3)
P 		66.6
(-3)
P 		69.7
(-2.8)
P 		107.7
(-0.9)
P 		91.2
(-1.8)
P 		119
B 205.6
(3)
P 		174.8
(1.2)
P 		105
(-2.8)
P 		100.9
(-3)
P 		131
(-1.3)
P 		137.9
(-0.9)
P 		105
C 268.3
(3)
P 		148.2
(-0.5)
P 		89.5
(-2.2)
P 		62.8
(-3)
P 		122.6
(-1.3)
P 		127.9
(-1.1)
P 		206
avr 182.9
(3) 		169.4
(2.2) 		87
(-2.5) 		77.8
(-3) 		120.4
(-0.6) 		119
(-0.6) 		105
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 171802_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.27656
Sequence Type Exemplar sequence
Representative Public ID C33749 NCBI
Target Description C33749_rc /REP_DB=TREMBL Accession /GB=C33749 /FEA=Transcript Cluster /DEF=C.elegans cDNA clone yk374b1 : 3prime end, single read.
Cluster Evidence This cluster is supported by a Transcript Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_067195(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_067195 NCBI Caenorhabditis elegans Y75B8A.24 (Y75B8A.24) mRNA, complete cds. 11/11 A 
SNAP00000020087 ENSEMBL cdna:SNAP chromosome:CEL140:III:12251572:12265841:-1 11/11 None
GENEFINDER00000020179 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:12251572:12256888:-1 11/11 None
Y75B8A.24 ENSEMBL cdna:known chromosome:CEL140:III:12251292:12273555:-1 gene:Y75B8A.24 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:12251395-12253354(-) 97.49 97.49
Public Domain and Genome References
Gene Title Phosphoinositide 3-kinase family, accessory domain (PIK domain) ; Phosphatidylinositol 3- and 4-kinases
Ensembl Y75B8A.24
Entrez Gene 176653 Entrez gene
SwissProt Q9XW63 EMBL-EBI
WormBase CE23035 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:262387_S_AT phosphatidylinositol 3- and 4-kinase family protein at
ATGENOME1:18182_S_AT phosphatidylinositol 3- and 4-kinase family protein at
CANINE_2:CFAAFFX.23481.1.S1_S_AT similar to phosphatidylinositol 4-kinase, catalytic, alpha polypeptide isoform 2 cfa
DROSOPHILA_2:1631334_AT dm
DROSGENOME1:144414_AT dm
CHICKEN:GGAAFFX.924.1.S1_AT similar to phosphatidylinositol 4-kinase, catalytic, alpha polypeptide isoform 2; phosphatidylinositol 4-kinase, type II, alpha; phosphatidylinositol 4-kinase, type III, alpha; phosphatidylinositol 4-kinase 230 gga
HG-U133_PLUS_2:207081_S_AT phosphatidylinositol 4-kinase, catalytic, alpha polypeptide hs
HG-U133A:207081_S_AT phosphatidylinositol 4-kinase, catalytic, alpha polypeptide hs
HG-U133A_2:207081_S_AT phosphatidylinositol 4-kinase, catalytic, alpha polypeptide hs
HU35KSUBD:RC_AA421269_AT phosphatidylinositol 4-kinase, catalytic, alpha polypeptide hs
HUGENEFL:L36151_AT phosphatidylinositol 4-kinase, catalytic, alpha polypeptide hs
U133_X3P:G4505806_3P_X_AT phosphatidylinositol 4-kinase, catalytic, alpha polypeptide hs
U133_X3P:G4505806_3P_A_AT phosphatidylinositol 4-kinase, catalytic, alpha polypeptide hs
HG-U133A_2:213408_S_AT phosphatidylinositol 4-kinase, catalytic, alpha polypeptide hs
HG-FOCUS:213408_S_AT phosphatidylinositol 4-kinase, catalytic, alpha polypeptide hs
HG-U133_PLUS_2:213408_S_AT phosphatidylinositol 4-kinase, catalytic, alpha polypeptide hs
HG-U133A:213408_S_AT phosphatidylinositol 4-kinase, catalytic, alpha polypeptide hs
HG-U95AV2:40783_S_AT phosphatidylinositol 4-kinase, catalytic, alpha polypeptide hs
HG-U95E:71216_I_AT phosphatidylinositol 4-kinase, catalytic, alpha polypeptide hs
HG-U95E:71218_F_AT phosphatidylinositol 4-kinase, catalytic, alpha polypeptide hs
MG-U74CV2:169000_AT phosphatidylinositol 4-kinase, catalytic, alpha polypeptide mm
MU11KSUBB:MSA.10716.0_S_AT phosphatidylinositol 4-kinase, catalytic, alpha polypeptide mm
MG-U74AV2:104208_AT phosphatidylinositol 4-kinase, catalytic, alpha polypeptide mm
MG-U74BV2:164881_F_AT phosphatidylinositol 4-kinase, catalytic, alpha polypeptide mm
MOE430A:1435003_AT phosphatidylinositol 4-kinase, catalytic, alpha polypeptide mm
MOUSE430A_2:1435003_AT phosphatidylinositol 4-kinase, catalytic, alpha polypeptide mm
MOUSE430_2:1435003_AT phosphatidylinositol 4-kinase, catalytic, alpha polypeptide mm
MG-U74CV2:166846_R_AT Phosphatidylinositol 4-kinase, catalytic, alpha polypeptide (Pik4ca), mRNA mm
MU19KSUBA:TC16406_AT Phosphatidylinositol 4-kinase, catalytic, alpha polypeptide (Pik4ca), mRNA mm
MU19KSUBA:TC16509_AT Phosphatidylinositol 4-kinase, catalytic, alpha polypeptide (Pik4ca), mRNA mm
MU19KSUBA:TC16509_G_AT Phosphatidylinositol 4-kinase, catalytic, alpha polypeptide (Pik4ca), mRNA mm
RT-U34:D83538_G_AT phosphatidylinositol 4-kinase, catalytic, alpha polypeptide rn
RG-U34A:D83538_G_AT phosphatidylinositol 4-kinase, catalytic, alpha polypeptide rn
RT-U34:D83538_AT phosphatidylinositol 4-kinase, catalytic, alpha polypeptide rn
RG-U34A:D83538_AT phosphatidylinositol 4-kinase, catalytic, alpha polypeptide rn
RG-U34A:U39572_S_AT phosphatidylinositol 4-kinase, catalytic, alpha polypeptide rn
RG-U34C:RC_AI146154_S_AT phosphatidylinositol 4-kinase, catalytic, alpha polypeptide rn
RAE230A:1370318_AT phosphatidylinositol 4-kinase, catalytic, alpha polypeptide rn
RAT230_2:1370318_AT phosphatidylinositol 4-kinase, catalytic, alpha polypeptide rn
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
7018 microtubule-based movement inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
5874 microtubule inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4428 inositol or phosphatidylinositol kinase activity inferred from electronic annotation QuickGO AmiGO
5198 structural molecule activity inferred from electronic annotation QuickGO AmiGO
16773 phosphotransferase activity, alcohol group as acceptor inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA22108 Hypothetical protein Y75B8A.24 [Caenorhabditis elegans] ref|NP_499596.1| Y75B8A.24 [Caenorhabditis elegans] 0.0
blast CAE60054 Hypothetical protein CBG03566 [Caenorhabditis briggsae] 0.0
blast NP_001030144 hypothetical protein LOC556956 [Danio rerio] gb|AAY16567.1| phosphatidylinositol 4-kinase III alpha [Danio rerio] 0.0
blast CAA22108 Hypothetical protein Y75B8A.24 [Caenorhabditis elegans] ref|NP_499596.1| Y75B8A.24 [Caenorhabditis elegans] 1.0E-166
Protein Domains
Database ID Description E-Value
Pfam IPR000403 EMBL-EBI Phosphatidylinositol 3- and 4-kinase 5.4E-68
Pfam IPR001263 EMBL-EBI Phosphoinositide 3-kinase accessory domain PIK 2.1E-25
Pfam IPR000403 EMBL-EBI Phosphatidylinositol 3- and 4-kinase 5.4E-68
Pfam IPR000403 EMBL-EBI Phosphatidylinositol 3- and 4-kinase 5.4E-68
Pfam IPR001263 EMBL-EBI Phosphoinositide 3-kinase accessory domain PIK 2.1E-25
Sequence
Target Sequence 
>C. ELEGANS:171802_S_AT
ccctatcacaaatcattcgtttcacttgtttcattgatgttggacacacatttgccatgt
tttagaggnnnnnctattaatcaactgagagccagatttgcacctgaaatgaatgnnnnn
gatgcggcgaaatacatgtactcggtgatcaccaactgcttcc

BLASTn GenBank NR
Note: "n"'s represent regions that are not probed by the probe sequences.
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CCCTATCACAAATCATTCGTTTCAC 577 259 13 Antisense
AAATCATTCGTTTCACTTGTTTCAT 242 117 22 Antisense
CATTCGTTTCACTTGTTTCATTGAT 696 215 26 Antisense
TTCACTTGTTTCATTGATGTTGGAC 378 687 33 Antisense
GATGTTGGACACACATTTGCCATGT 472 409 48 Antisense
TGTTGGACACACATTTGCCATGTTT 59 563 50 Antisense
TCAACTGAGAGCCAGATTTGCACCT 441 629 93 Antisense
GAGAGCCAGATTTGCACCTGAAATG 347 395 99 Antisense
GCCAGATTTGCACCTGAAATGAATG 244 281 103 Antisense
ATACATGTACTCGGTGATCACCAAC 458 23 144 Antisense
TACTCGGTGATCACCAACTGCTTCC 181 647 151 Antisense
Sequence Source Affymetrix Proprietary Database