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[171741_x_at]

Description CEC1925 /REP_DB=TREMBL Accession /5_PRIME_EXT_ID=C44B12.5 /5_PRIME_EXT_DB=WormBase Gene ID /GB=C13192 /WB_GENE_ID=C44B12.5 /WP=CE16921 /CHR=4 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk165d1 : 5prime end, single read.
Cluster 6grp_total > 99      4grp_total > 42      6grp_total_sub > Increase      6grp_rule_based > S5      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 600
1 2 3 4 range
A 254.9
(-3)
M 		854.9
(3)
A 		469.3
(-0.9)
A 		416.9
(-1.4)
P 		600
B 285.1
(-3)
A 		845.7
(3)
A 		660.9
(1)
A 		441.1
(-1.3)
P 		561
C 290.3
(-3)
A 		634.7
(3)
A 		378.7
(-1.5)
A 		429.5
(-0.6)
P 		344
avr 276.8
(-3) 		778.4
(3) 		503
(-0.3) 		429.2
(-1.2) 		502

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 4970
1 2 3 4 5 6 range
A 230.3
(-3)
A 		273.8
(-2.9)
A 		250.3
(-3)
A 		310.3
(-2.9)
A 		4690.2
(3)
P 		4157.1
(2.3)
P 		4460
B 282.1
(-3)
A 		402.4
(-2.8)
A 		249.7
(-3)
A 		289.2
(-2.9)
A 		4571.4
(3)
P 		3327.3
(1.3)
P 		4322
C 233.6
(-3)
A 		266
(-3)
A 		346.3
(-2.9)
A 		316.3
(-2.9)
P 		5199.9
(3)
P 		2286.9
(-0.5)
P 		4966
avr 248.7
(-3) 		314.1
(-2.9) 		282.1
(-3) 		305.3
(-2.9) 		4820.5
(3) 		3257.1
(0.9) 		4572
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 148
1 2 3 4 range
A 92.8
(-1.5)
M 		185.6
(3)
A 		122.7
(-0)
A 		61
(-3)
P 		125
B 90.9
(-3)
A 		208.9
(3)
A 		153.1
(0.2)
A 		94
(-2.8)
P 		118
C 96.1
(-1.7)
A 		182.5
(3)
A 		132
(0.2)
A 		72.4
(-3)
P 		110
avr 93.3
(-2.1) 		192.3
(3) 		135.9
(0.1) 		75.8
(-3) 		117

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1815
1 2 3 4 5 6 range
A 42.4
(-3)
A 		97.4
(-2.8)
A 		54.5
(-2.9)
A 		78.7
(-2.8)
A 		1415.3
(3)
P 		1012.1
(1.2)
P 		1373
B 98.2
(-2.9)
A 		134
(-2.8)
A 		60.6
(-3)
A 		76.6
(-2.9)
A 		1857.3
(3)
P 		1240.7
(0.9)
P 		1797
C 86.6
(-2.9)
A 		81.2
(-2.9)
A 		88.1
(-2.9)
A 		63.6
(-3)
P 		1682.6
(3)
P 		765.6
(-0.4)
P 		1619
avr 75.7
(-3) 		104.2
(-2.9) 		67.7
(-3) 		73
(-3) 		1651.7
(3) 		1006.1
(0.6) 		1584
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 171741_x_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.24997
Sequence Type Exemplar sequence
Representative Public ID CEC1925 NCBI
Target Description CEC1925 /REP_DB=TREMBL Accession /5_PRIME_EXT_ID=C44B12.5 /5_PRIME_EXT_DB=WormBase Gene ID /GB=C13192 /WB_GENE_ID=C44B12.5 /WP=CE16921 /CHR=4 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk165d1 : 5prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_067640(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_067640 NCBI Caenorhabditis elegans C44B12.5 (C44B12.5) mRNA, complete cds. 11/11 A 
SNAP00000002999 ENSEMBL cdna:SNAP chromosome:CEL140:IV:1121582:1125127:-1 11/11 None
GENEFINDER00000003007 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:1121582:1125085:-1 11/11 None
C44B12.5 ENSEMBL cdna:known chromosome:CEL140:IV:1121495:1125283:-1 gene:C44B12.5 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:1121518-1125290(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl C44B12.5
Entrez Gene 176932 Entrez gene
SwissProt O44144 EMBL-EBI
WormBase CE16921 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast NP_500041 C44B12.5 [Caenorhabditis elegans] gb|AAB88328.1| Hypothetical protein C44B12.5 [Caenorhabditis elegans] 1.0E-128
blast NP_500041 C44B12.5 [Caenorhabditis elegans] gb|AAB88328.1| Hypothetical protein C44B12.5 [Caenorhabditis elegans] 1.0E-105
blast NP_500041 C44B12.5 [Caenorhabditis elegans] gb|AAB88328.1| Hypothetical protein C44B12.5 [Caenorhabditis elegans] 5.0E-96
blast CAE68279 Hypothetical protein CBG13962 [Caenorhabditis briggsae] 5.0E-60
blast CAE68279 Hypothetical protein CBG13962 [Caenorhabditis briggsae] 6.0E-60
blast CAE68279 Hypothetical protein CBG13962 [Caenorhabditis briggsae] 7.0E-60
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_500041.1 1 23-45
C44B12.5 1 23-45
Sequence
Target Sequence 
>C. ELEGANS:171741_X_AT
gactctacctacttgtcagaggccaactgggagccaaagaaatatcataagaatgtcaag
tgcgtgtgctgtgctccaagatcctctccagctgctcagaacccatcctacaacaacaat
gctgctcaatccccaccaactggctacaacaacaacgccaacgccttcgtccagccacaa
ccagtagctgctcagtccccaccaatgtcagcctcccagccactcgttgacctctggcaa
tccgagaatactgctccatcaacatcatggggagctgctccagctgctccatcaacatca
tggggagctgctccagctgctccaccaacctggggaggtgctccaactgctgctccaact
tggggagccgccaatgatgctccaccagcggccacgccggctccagttgctgcttccaat
gcttatta

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GACTCTACCTACTTGTCAGAGGCCA 195 373 779 Antisense
AATGTCAAGTGCGTGTGCTGTGCTC 97 165 830 Antisense
ACAACAACAATGCTGCTCAATCCCC 699 111 888 Antisense
GTCCAGCCACAACCAGTAGCTGCTC 479 477 947 Antisense
TCGTTGACCTCTGGCAATCCGAGAA 602 603 1002 Antisense
AGAATACTGCTCCATCAACATCATG 524 75 1023 Antisense
TCCAGCTGCTCCATCAACATCATGG 676 589 1057 Antisense
ATCATGGGGAGCTGCTCCAGCTGCT 533 19 1075 Antisense
GCTCCACCAACCTGGGGAGGTGCTC 310 297 1097 Antisense
GCTGCTCCAACTTGGGGAGCCGCCA 18 297 1127 Antisense
CAGTTGCTGCTTCCAATGCTTATTA 293 205 1182 Antisense
Sequence Source Affymetrix Proprietary Database