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Cluster Members
Consensus/Exemplar

GeneChip Array Information

Probe Set ID

192323_at

GeneChip Array

C. elegans Genome Array

Organism Common Name

Nematode

Probe Design Information

Transcript ID(Array Design)

affy.Ce.8562

Sequence Type

Exemplar sequence

Representative Public ID

F38E11.2 NCBI

Target Description

F38E11.2 /REP_DB=WormBase Gene ID /WP=CE03294 /GEN=hsp-12.6 /TR=Q20165 /GB=CAA92771.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation /DEF=alpha-B-crystallin

Cluster Evidence

This cluster is supported by a Sanger Annotation.

Annotation Method Description

Annotation Description

This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).

Annotation Grade

This is a grade A annotation.

Annotation Transcript Cluster (# of Matching Probes)

NM_069267(11)

Transcript Assignments

Representative Transcript	Description	Matching Probes	Related Probesets by Grade
NM_069267 NCBI	Caenorhabditis elegans F38E11.2 (alpha-B-crystallin) mRNA, complete cds.	11/11	None
SNAP00000022710 ENSEMBL	cdna:SNAP chromosome:CEL140:IV:9446362:9446861:-1	11/11	None
GENEFINDER00000022722 ENSEMBL	cdna:GeneFinder chromosome:CEL140:IV:9446362:9446861:-1	11/11	None
F38E11.2 ENSEMBL	cdna:known chromosome:CEL140:IV:9446362:9446861:-1 gene:F38E11.2	11/11	None

Annotation Notes

There are no noteworthy cross hybridizing mRNAs found for this probe set.

Genomic Alignment of Consensus/Exemplar Sequence

Assembly

Wormbase March 2004

Alignment(s)

Position	Identity	Coverage	Cytoband
chrIV:9446367-9446867(-)	100.0	100.0

Public Domain and Genome References

Gene Title

alpha-B-crystallin

Gene Symbol

hsp-12.6

Ensembl

F38E11.2

Entrez Gene

177778 Entrez gene

SwissProt

Q20165 EMBL-EBI

WormBase

CE03294 Wormbase

Functional Annotations

Protein Similarities

Method	ID	Description	E-Value
blast	CAA92771	Hypothetical protein F38E11.2 [Caenorhabditis elegans] ref\|NP_501668.1\| F38E11.2 [Caenorhabditis elegans] gb\|AAC47521.1\| heat shock protein 12.6 [Caenorhabditis elegans]	1.0E-51
blast	CAE74012	Hypothetical protein CBG21660 [Caenorhabditis briggsae]	5.0E-44
blast	CAA92770	Hypothetical protein F38E11.1 [Caenorhabditis elegans] ref\|NP_501667.1\| F38E11.1 [Caenorhabditis elegans]	2.0E-31

Protein Domains

Database	ID	Description	E-Value
Pfam	IPR002068 EMBL-EBI	Heat shock protein Hsp20	1.5E-9

Sequence

Target Sequence

>C. ELEGANS:192323_AT
gattggccacttcaaaagggagatggagttgtcaatgtcctcgacgacgatgatcacttc
gaagtaggcctagaagcccataacttcctcccaaaagagattgaagtcaagaacattgga
gaacttcttgaaatccatatggagcacaatgtaaagaaggattcatttggagatgtctct
cgtaacattactcgttgttacaaacttccaaagaatgttgatatgaagacaatcaagagc
aacttggattcacatggaat

BLASTn GenBank NR

Probe Info

Probe Sequence(5'-3')	Probe X	Probe Y	Probe Interrogation Position	Strandedness
GATTGGCCACTTCAAAAGGGAGATG	337	435	55	Antisense
GATGGAGTTGTCAATGTCCTCGACG	381	407	76	Antisense
GTCCTCGACGACGATGATCACTTCG	217	477	91	Antisense
GATGATCACTTCGAAGTAGGCCTAG	599	409	103	Antisense
GTAGGCCTAGAAGCCCATAACTTCC	64	331	118	Antisense
GCCCATAACTTCCTCCCAAAAGAGA	494	283	130	Antisense
GAACTTCTTGAAATCCATATGGAGC	612	349	175	Antisense
GGATTCATTTGGAGATGTCTCTCGT	478	509	213	Antisense
GATGTCTCTCGTAACATTACTCGTT	120	409	226	Antisense
CATTACTCGTTGTTACAAACTTCCA	25	213	240	Antisense
AGAGCAACTTGGATTCACATGGAAT	700	69	290	Antisense

Sequence Source

Affymetrix Proprietary Database

Description	F38E11.2 /REP_DB=WormBase Gene ID /WP=CE03294 /GEN=hsp-12.6 /TR=Q20165 /GB=CAA92771.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation /DEF=alpha-B-crystallin
Cluster	6grp_total > 87 4grp_total > 02 6grp_cgc > 59 6grp_cgc_sub > Da 6grp_total_sub > Increase 6grp_rule_based > S6 4grp_rule_based > L4